LOCUS       40924_41525        4428 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV-Sport-HIF-Rluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4428)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4428)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..331
                     /label=5' UTR of mHIF-1alpha (IRES)
                     /note="5' UTR of mHIF-1alpha (IRES)"
     CDS             332..1264
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     promoter        complement(1357..1375)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1464..1503
                     /label=intron SV40 small t-antigen
                     /note="intron SV40 small t-antigen"
     polyA_signal    1762..1896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2133..2711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2885..3742)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3743..3847)
                     /label=AmpR promoter
     promoter        3959..3990
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3998..4014
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        4049..4378
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        4384..4402
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aattcctcga ctctagaaag cagggtggta acaacgcaga gtacgcgggg aagcctcctc
       61 ggctgagggg acgcgaggac tgtcctcgcc gccgtcgcgg gcagtgtcta gccaggcctt
      121 gacaagctag ccggaggagc gcctaggaac ccgagccgga gctcagcgag cgcagcctgc
      181 agctcccgcc tcgccgtccc ggggggcgtc ccgcctccca ccccgcctct ggacttgtct
      241 ctttctccgc gcgcgcggac agagcgggcg cttaggcccg agcgagcccg ggggccgccg
      301 gccgggaaga caacgcgggc accgattcgc catggcttcg aaagtttatg atccagaaca
      361 aaggaaacgg atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct
      421 tgattcattt attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt
      481 acatggtaac gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt
      541 agcgcggtgt attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg
      601 ttcttatagg ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt
      661 accaaagaag atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag
      721 ctatgagcat caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat
      781 tgaatcatgg gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga
      841 aggagaaaaa atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat
      901 catgagaaag ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg
      961 tgaagttcgt cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa
     1021 acctgacgtt gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt
     1081 accaaaaatg tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc
     1141 caagaagttt cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga
     1201 tgcacctgat gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga
     1261 acaataagaa ttcccgggtc gacgagctca ctagtcggcg gccgctctag aggatccaag
     1321 cttacgtacg cgtgcatgcg acgtcatagc tctctcccta tagtgagtcg tattataagc
     1381 tagcttggga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     1441 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     1501 ctagctgcat atgcttgctg cttgagagtt ttgcttactg agtatgattt atgaaaatat
     1561 tatacacagg agctagtgat tctaattgtt tgtgtatttt agattcacag tcccaaggct
     1621 catttcaggc ccctcagtcc tcacagtctg ttcatgatca taatcagcca taccacattt
     1681 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1741 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1801 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1861 tccaaactca tcaatgtatc ttatcatgtc tggatcctgc attaatgaat cggccaacgc
     1921 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1981 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2041 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2101 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2161 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2221 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2281 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     2341 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2401 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2461 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2521 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2581 tttggtatct gcggccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2641 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2701 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2761 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2821 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2881 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2941 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3001 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3061 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3121 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3181 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3241 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3301 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3361 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3421 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3481 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3541 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3601 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3661 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3721 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3781 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3841 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3901 cattaaccta taaaaatagg cgtatacgag gccctttcac tcattaggca ccccaggctt
     3961 tacactttat agcttccggc tcgtataatg tgtggaattg tgagcggata acaatttcac
     4021 acaggaaaca gcatcgatgc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4081 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4141 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4201 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4261 ttctccgccc catggcgtac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4321 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4381 gctatttagg tgacactata gaaggtacgc ctgcaggtac cggtccgg
//
