LOCUS       pSV-SPORT.        3169 bp DNA     circular SYN 03-FEB-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pSV-SPORT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Tontonoz P, Kim JB, Graves RA, Spiegelman BM
  TITLE     ADD1: a novel helix-loop-helix transcription factor associated with 
            adipocyte determination and differentiation.
  JOURNAL   Mol Cell Biol. 1993 Aug . 13(8):4753-9.
  PUBMED    8336713
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1993 Aug . 13(8):4753-9."
FEATURES             Location/Qualifiers
     source          1..3169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..338
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        344..362
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(477..495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    882..1016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1253..1841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2015..2872)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2873..2977)
                     /label=AmpR promoter
     promoter        3090..3120
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3128..3144
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        3169
                     /label=SV40
                     /note="Simian virus 40 enhancer/early promoter"
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aatccccagg ctccccagca ggcagaagta
       61 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      181 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      241 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      301 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctatttagg tgacactata
      361 gaaggtacgc ctgcaggtac cggtccggaa ttcccgggtc gacgagctca ctagtcggcg
      421 gccgctctag aggatccaag cttacgtacg cgtgcatgcg acgtcatagc tctctcccta
      481 tagtgagtcg tattataagc tagcttggga tctttgtgaa ggaaccttac ttctgtggtg
      541 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
      601 taagtgtata atgtgttaaa ctagctgcat atgcttgctg cttgagagtt ttgcttactg
      661 agtatgattt atgaaaatat tatacacagg agctagtgat tctaattgtt tgtgtatttt
      721 agattcacag tcccaaggct catttcaggc ccctcagtcc tcacagtctg ttcatgatca
      781 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
      841 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcctgc
     1021 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1681 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1921 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1981 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2041 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2101 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2161 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2221 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2281 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2341 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2401 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2461 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2521 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2581 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2641 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2701 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2761 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2821 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2881 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2941 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3001 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3061 ggccctttca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     3121 tgtggaattg tgagcggata acaatttcac acaggaaaca gcatcgatg
//
