LOCUS       40924_41475        4828 bp DNA     circular SYN 18-DEC-2018
DEFINITION  SiRNA vector pSUPER-hSyn-EGFP-CytB-AS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Michel U, Malik I, Ebert S, Bahr M, Kugler S.
  TITLE     Long-term in vivo and in vitro AAV-2-mediated RNA interference in 
            rat retinal ganglion cells and cultured primary neurons
  JOURNAL   Biochem. Biophys. Res. Commun. 326 (2), 307-312 (2005)
  PUBMED    15582578
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Michel U, Kuegler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2004) Neurology, University of Goettingen, Waldweg
            33, Goettingen 37073, Germany
REFERENCE   3  (bases 1 to 4828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2005"; volume: "326"; issue: "2"; 
            pages: "307-312"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2004) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen 37073, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(707..841)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(857..990)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(1005..1721)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    complement(1740..1758)
                     /note="MCS; multiple cloning site"
     promoter        complement(1764..2211)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     promoter        2239..2453
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    2455..2586
                     /note="Cytb-AS; partial human cytochrome B antisense
                     sequence"
     primer_bind     complement(2594..2610)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2640..2658)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2679..2695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2703..2719)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2727..2757)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2772..2793)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3081..3669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3843..4700)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4701..4805)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcttcgatg ctagacgatc cagacatgat
      721 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
      781 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      841 tgctcgaagt cgacacctgt ggagagaaag gcaaagtgga tgtcagtaag accaataggt
      901 gcctatcaga aacgcaagag tcttctctgt ctcgacaagc ccagtttcta tgtggtctcc
      961 ttaaacctgt cttgtaacct tgatacttac ctgcggccgc tttacttgta cagctcgtcc
     1021 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     1081 tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc
     1141 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     1201 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
     1261 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
     1321 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     1381 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     1441 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     1501 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
     1561 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     1621 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     1681 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac cggtggatcc
     1741 ttgctagcag cttgaattct cgactgcgct ctcaggcacg acacgactcc tccgctgccc
     1801 accgcagact gaggcagcgc tgagtcgccg gcgccgcagc gcagatggtc gcgcccgtgc
     1861 ccccctatct cgcgcctcgc gtggtgcggt ccggctgggc cggcggcggc gcggacgcga
     1921 ccaaggtggc cgggaagggg agtttgcggg ggaccggcga gtgacgtcag cgcgccttca
     1981 gtgctgaggc ggcggtggcg cgcgccgcca ggcgggggcg aaggcactgt ccgcggtgct
     2041 gaagctggca gtgcgcacgc gcctcgccgc atcctgtttc ccctccccct ctctgatagg
     2101 ggatgcgcaa tttggggaat gggggttggg tgcttgtcca gtgggtcggg gtcggtcgtc
     2161 aggtaggcac ccccaccccg cctcatcctg gtcctaaaac ccacttgcac tcatacgcag
     2221 ggccctctgc aggaattcga acgctgacgt catcaacccg ctccaaggaa tcgcgggccc
     2281 agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg
     2341 gacaggggag tggcgccctg caatatttgc atgtcgctat gtgttctggg aaatcaccat
     2401 aaacgtgaaa tgtctttgga tttgggaatc ttataagttc tgtatgagac cacagatctc
     2461 ccaatgtatg ggatggcgga tagtaagttt gtaattactg tggcccctca gaatgatatt
     2521 tggcctcacg ggaggacata gcctatgaag gctgttgcta tagttgcaag caggaggata
     2581 atgccaagct tatcgatacc gtcgacctcg agggggggcc cggtacccag cttttgttcc
     2641 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     2701 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2761 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2821 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2881 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2941 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3001 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3061 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3121 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3181 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3241 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3301 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3361 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3421 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3481 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3541 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3601 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3661 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3721 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3781 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3841 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3901 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3961 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4021 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4081 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4141 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4201 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4261 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4321 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4381 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4441 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4501 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4561 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4621 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4681 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4741 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4801 ccgcgcacat ttccccgaaa agtgccac
//
