LOCUS       40924_41455        4906 bp DNA     circular SYN 18-DEC-2018
DEFINITION  SiRNA vector pSUPER-CMV-DsRed2N1-CytB-AS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Michel U, Malik I, Ebert S, Bahr M, Kugler S.
  TITLE     Long-term in vivo and in vitro AAV-2-mediated RNA interference in 
            rat retinal ganglion cells and cultured primary neurons
  JOURNAL   Biochem. Biophys. Res. Commun. 326 (2), 307-312 (2005)
  PUBMED    15582578
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Michel U, Kuegler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2004) Neurology, University of Goettingen, Waldweg
            33, Goettingen 37073, Germany
REFERENCE   3  (bases 1 to 4906)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4906)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2005"; volume: "326"; issue: "2"; 
            pages: "307-312"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2004) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen 37073, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     681..697
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(752..886)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(902..1035)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(1048..1722)
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    complement(1741..1760)
                     /note="MCS'; multiple cloning site"
     promoter        complement(1763..2291)
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    2294..2309
                     /note="MCS'; multiple cloning site"
     promoter        2317..2531
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    2533..2664
                     /note="Cytb-AS; partial human cytochrome B antisense
                     sequence"
     primer_bind     complement(2672..2688)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2718..2736)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2757..2773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2781..2797)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2805..2835)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2850..2871)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3159..3747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3921..4778)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4779..4883)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccccccct cgaggtcgac ggtatcgata agctagcttg atatcgaatt
      721 cctgcagccc gggggatctt cgatgctaga cgatccagac atgataagat acattgatga
      781 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      841 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttgctc gaagtcgaca
      901 cctgtggaga gaaaggcaaa gtggatgtca gtaagaccaa taggtgccta tcagaaacgc
      961 aagagtcttc tctgtctcga caagcccagt ttctatgtgg tctccttaaa cctgtcttgt
     1021 aaccttgata cttacctgcg gccgctacag gaacaggtgg tggcggccct cggtgcgctc
     1081 gtactgctcc acgatggtgt agtcctcgtt gtgggaggtg atgtccagct tggcgtccac
     1141 gtagtagtag ccgggcagct gcacgggctt cttggccatg tagatggact tgaactccac
     1201 caggtagtgg ccgccgtcct tcagcttcag ggccttgtgg gtctcgccct tcagcacgcc
     1261 gtcgcggggg tacaggcgct cggtggaggc ctcccagccc atggtcttct tctgcatcac
     1321 ggggccgtcg gaggggaagt tcacgccgat gaacttcacc ttgtagatga agcagccgtc
     1381 ctgcagggag gagtcctggg tcacggtcgc cacgccgccg tcctcgaagt tcatcacgcg
     1441 ctcccacttg aagccctcgg ggaaggacag cttcttgtag tcggggatgt cggcggggtg
     1501 cttcacgtac accttggagc cgtactggaa ctggggggac aggatgtccc aggcgaaggg
     1561 cagggggccg cccttggtca ccttcagctt cacggtgttg tggccctcgt aggggcggcc
     1621 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gtgccctcca tgcgcacctt
     1681 gaagcgcatg aactcggtga tgacgttctc ggaggaggcc atggtggcga ccggtggatc
     1741 cttgctagca gcttgaattc gatctgcgtt ctacggtggt cagaccgaag actgcgacgg
     1801 taccgacgct ggtcgcgcct cttataccca cgtagaacgc agctcagcca atagaatgag
     1861 tgccaatatg gaatttccag gggaaaaccg gggcggtgtt acgttttggc tgccctttca
     1921 cttcccattg acgtgtattg gctcgagaac ggtactttcc cattaatcag ctatgggaaa
     1981 gtaccgttta aaggtcacgt tgcattagtt tcaatagccc attgacgtca atggtgggaa
     2041 agtacatggc gttttaatta aatggctgga aaaacccaat gactcacccc tattgacctt
     2101 atgtacgtgc caataatggg aaaaacccat tgactcaccc cctattgacc ttttgtactg
     2161 ggcaaaaccc aatggaaagt ccctattgac tcagtgtact tggctccaat gggactttcc
     2221 tgttgattca cccctattga ccttatgtac tgggcaaaac ccattggaaa gtccctaatg
     2281 actcagtata tctctagagt cgacctgcag gaattcgaac gctgacgtca tcaacccgct
     2341 ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc
     2401 aggaagatgg ctgtgaggga caggggagtg gcgccctgca atatttgcat gtcgctatgt
     2461 gttctgggaa atcaccataa acgtgaaatg tctttggatt tgggaatctt ataagttctg
     2521 tatgagacca cagatctccc aatgtatggg atggcggata gtaagtttgt aattactgtg
     2581 gcccctcaga atgatatttg gcctcacggg aggacatagc ctatgaaggc tgttgctata
     2641 gttgcaagca ggaggataat gccaagctta tcgataccgt cgacctcgag ggggggcccg
     2701 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     2761 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2821 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2881 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2941 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3001 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3901 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3961 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4021 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4081 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4141 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4201 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4261 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4321 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4381 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4441 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4501 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4561 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4621 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4681 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4741 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4801 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4861 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
