LOCUS       40924_41450       11597 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSUP81-ZA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Zehner S, Schober G, Wenzel M, Lang K, Gottfert M.
  TITLE     Expression of the Bradyrhizobium japonicum type III secretion system
            in legume nodules and analysis of the associated tts box promoter
  JOURNAL   Mol. Plant Microbe Interact. 21 (8), 1087-1093 (2008)
  PUBMED    18616405
REFERENCE   2  (bases 1 to 11597)
  AUTHORS   Zehner S, Goettfert M.
  TITLE     Mobilizable vector pSUP81-ZA for reporter gene fusions with lacZ and
            uidA gene
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11597)
  AUTHORS   Zehner S, Goettfert M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2007) Institute of Genetics, University of 
            Technology Dresden, Helmholtzstrasse 10, Dresden D-01062, Germany
REFERENCE   4  (bases 1 to 11597)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11597)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant 
            Microbe Interact."; date: "2008"; volume: "21"; issue: "8"; pages: 
            "1087-1093"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2007) Institute of Genetics, University of Technology 
            Dresden, Helmholtzstrasse 10, Dresden D-01062, Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             58..1245
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             1889..2077
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    2182..2322
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2508..3096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3593..3631
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             3656..6700
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAAFQLSAGRYHYQLVWCQK"
     CDS             6820..8628
                     /codon_start=1
                     /gene="uidA"
                     /product="beta-glucuronidase"
                     /label=uidA
                     /note="UidA"
                     /protein_id="ACA50288.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            6820..8628
                     /gene="uidA"
                     /label=uidA
     CDS             complement(9818..10186)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(10219..10328)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
       61 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata
      121 ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc
      181 gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc
      241 gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt
      301 ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat cctctacgcc
      361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
      421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
      481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
      541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
      601 caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc
      661 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
      721 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
      781 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
      841 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
      901 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
      961 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     1021 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     1081 gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc
     1141 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     1201 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     1261 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
     1321 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
     1381 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
     1441 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
     1501 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     1561 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
     1621 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac
     1681 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc
     1741 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat
     1801 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca
     1861 gaaattcccc cttacacgga ggcatcaagt gaccaaacag gaaaaaaccg cccttaacat
     1921 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
     1981 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
     2041 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     2101 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     2161 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     2221 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     2281 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     2341 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2401 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2461 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2521 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2581 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2641 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2701 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2761 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2821 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2881 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2941 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3001 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3061 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg cccaccgcgg tggcggccgc
     3601 tctagaacta gttaattaac gtgacaggcc tcgacctgca gccaagcttg ctcccgtcgt
     3661 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3721 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3781 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc
     3841 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg
     3901 gcagatgcac ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa
     3961 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga
     4021 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt
     4081 tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga
     4141 atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg
     4201 ttggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg
     4261 tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg
     4321 ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt
     4381 gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg
     4441 caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac
     4501 actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg
     4561 tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt
     4621 cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct
     4681 gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca
     4741 gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc
     4801 gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt
     4861 ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc
     4921 gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca
     4981 cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc
     5041 gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg
     5101 agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca
     5161 gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac
     5221 gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc
     5281 taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg
     5341 ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg
     5401 tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga
     5461 ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg
     5521 tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga
     5581 gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct
     5641 ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag
     5701 cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga
     5761 agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct
     5821 ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga
     5881 gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat
     5941 tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga
     6001 taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg
     6061 gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact
     6121 tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt
     6181 tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt
     6241 tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca
     6301 ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct
     6361 tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt
     6421 cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca
     6481 gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac
     6541 cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca
     6601 tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggcat tccagctgag
     6661 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc
     6721 atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttaaata actaactaaa
     6781 ggaggaacat atatgagctt ggctgcaggt cagtccctta tgttacgtcc tgtagaaacc
     6841 ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac
     6901 tgtggaattg atcagcgttg gtgggaaagc gcgttacaag aaagccgggc aattgctgtg
     6961 ccaggcagtt ttaacgatca gttcgccgat gcagatattc gtaattatgc gggcaacgtc
     7021 tggtatcagc gcgaagtctt tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt
     7081 ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt gatggagcat
     7141 cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc cgggaaaagt
     7201 gtacgtatca ccgtttgtgt gaacaacgaa ctgaactggc agactatccc gccgggaatg
     7261 gtgattaccg acgaaaacgg caagaaaaag cagtcttact tccatgattt ctttaactat
     7321 gccggaatcc atcgcagcgt aatgctctac accacgccga acacctgggt ggacgatatc
     7381 accgtggtga cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg
     7441 gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt tgcaactgga
     7501 caaggcacta gcgggacttt gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt
     7561 tatctctatg aactgtgcgt cacagccaaa agccagacag agtgtgatat ctacccgctt
     7621 cgcgtcggca tccggtcagt ggcagtgaag ggccaacagt tcctgattaa ccacaaaccg
     7681 ttctacttta ctggctttgg tcgtcatgaa gatgcggact tacgtggcaa aggattcgat
     7741 aacgtgctga tggtgcacga ccacgcatta atggactgga ttggggccaa ctcctaccgt
     7801 acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg
     7861 gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg tttcgaagcg
     7921 ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg
     7981 cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg
     8041 tggagtattg ccaacgaacc ggatacccgt ccgcaagtgc acgggaatat ttcgccactg
     8101 gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc
     8161 tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct gaaccgttat
     8221 tacggatggt atgtccaaag cggcgatttg gaaacggcag agaaggtact ggaaaaagaa
     8281 cttctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata cggcgtggat
     8341 acgttagccg ggctgcactc aatgtacacc gacatgtgga gtgaagagta tcagtgtgca
     8401 tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta
     8461 tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa
     8521 gggatcttca ctcgcgaccg caaaccgaag tcggcggctt ttctgctgca aaaacgctgg
     8581 actggcatga acttcggtga aaaaccgcag cagggaggca aacaatgaat caacaactct
     8641 cctggcgcac catcgtcggc tacagcctcg ggaattgctg caggtcgagg gggggcccgg
     8701 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8761 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8821 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8881 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactgcg
     8941 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
     9001 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     9061 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     9121 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     9181 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatccagc cgaccaggct
     9241 ttccacgccc gcgtgccgct ccatgtcgtt cgcgcggttc tcggaaacgc gctgccgcgt
     9301 ttcgtgattg tcacgctcaa gcccgtagtc ccgttcgagc gtcgcgcaga ggtcagcgag
     9361 ggcgcggtag gcccgatacg gctcatggat ggtgtttcgg gtcgggtgaa tcttgttgat
     9421 ggcgatatgg atgtgcaggt tgtcggtgtc gtgatgcacg gcactgacgc gctgatgctc
     9481 ggcgaagcca agcccagcgc agatgcggtc ctcaatcgcg cgcaacgtct ccgcgtcggg
     9541 cttctctccc gcgcggaagc taaccagcag gtgataggtc ttgtcggcct cggaacgggt
     9601 gttgccgtgc tgggtcgcca tcacctcggc catgacagcg ggcagggtgt ttgcctcgca
     9661 gttcgtgacg cgcacgtgac ccaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt
     9721 caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac
     9781 gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca
     9841 cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga
     9901 ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca
     9961 ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt
    10021 ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca
    10081 tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct
    10141 tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat
    10201 ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca
    10261 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
    10321 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
    10381 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga
    10441 agcagggtta tgcagcggaa aagcgctgct tccctgctgt tttgtggaat atctaccgac
    10501 tggaaacagg caaatgcagg aaattactga actgagggga caggcgagag acgatgccaa
    10561 agagctacac cgacgagctg gccgagtggg ttgaatcccg cgcggccaag aagcgccggc
    10621 gtgatgaggc tgcggttgcg ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt
    10681 ccggctatgc gctcgtcacc atttgggagc acatgcggga aacggggaag gtcaagttct
    10741 cctacgagac gttccgctcg cacgccaggc ggcacatcaa ggccaagccc gccgatgtgc
    10801 ccgcaccgca ggccaaggct gcggaacccg cgccggcacc caagacgccg gagccacggc
    10861 ggccgaagca ggggggcaag gctgaaaagc cggcccccgc tgcggccccg accggcttca
    10921 ccttcaaccc aacaccggac aaaaaggatc tactgtaatg gcgaaaattc acatggtttt
    10981 gcagggcaag ggcggggtcg gcaagtcggc catcgccgcg atcattgcgc agtacaagat
    11041 ggacaagggg cagacaccct tgtgcatcga caccgacccg gtgaacgcga cgttcgaggg
    11101 ctacaaggcc ctgaacgtcc gccggctgaa catcatggcc ggcgacgaaa ttaactcgcg
    11161 caacttcgac accctggtcg agctgattgc gccgaccaag gatgacgtgg tgatcctcta
    11221 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
    11281 cgccgacatc accgatgggg aagatccttg aagctgtccc tgatggtcgt catctacctg
    11341 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
    11401 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
    11461 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
    11521 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccatgttt
    11581 gacagcttat catcgat
//
