LOCUS       40924_41445        7830 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSUP202, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Rock JL, Nelson DR.
  TITLE     Identification and characterization of a hemolysin gene cluster in 
            Vibrio anguillarum
  JOURNAL   Infect. Immun. 74 (5), 2777-2786 (2006)
  PUBMED    16622215
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Nelson DR, Denkin SM.
  TITLE     Mobilizable vector plasmid pSUP202
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7830)
  AUTHORS   Nelson DR, Denkin SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2003) Cell and Molecular Biology, University of 
            Rhode Island, 117 Morrill Hall, Kingston, RI 02881, USA
REFERENCE   4  (bases 1 to 7830)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7830)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect. 
            Immun."; date: "2006"; volume: "74"; issue: "5"; pages: "2777-2786"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-2003) Cell and Molecular Biology, University of Rhode 
            Island, 117 Morrill Hall, Kingston, RI 02881, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        599..627
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             675..1862
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             2504..2692
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    2797..2937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3123..3713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3887..4744)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4745..4849)
                     /label=AmpR promoter
     promoter        5052..5154
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5155..5811
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(6669..7037)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(7070..7179)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gcagggcaag ggcggggtcg gcaagtcggc catcgccgcg atcattgcgc agtacaagat
       61 ggacaagggg cagacaccct tgtgcatcga caccgacccg gtgaacgcga cgttcgaggg
      121 ctacaaggcc ctgaacgtcc gccggctgaa catcatggcc ggcgacgaaa ttaactcgcg
      181 caacttcgac accctggtcg agctgattgc gccgaccaag gatgacgtgg tgatcctcta
      241 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
      301 cgccgacatc accgatgggg aagatccttg aagctgtccc tgatggtcgt catctacctg
      361 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
      421 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
      481 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
      541 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccatgttt
      601 gacagcttat catcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca
      661 gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc
      721 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg
      781 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc
      841 aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc
      901 tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc
      961 tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     1021 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     1081 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     1141 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     1201 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag
     1261 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     1321 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     1381 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     1441 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     1501 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     1561 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     1621 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     1681 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca
     1741 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
     1801 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     1861 cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     1921 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
     1981 gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg
     2041 ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
     2101 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
     2161 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
     2221 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
     2281 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac ccttgagtga
     2341 tttttctctg gtcccgccgc attcataccg ccagttggtt accctcacaa cgttccagta
     2401 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
     2461 tcattacccc catgaacaga aatccccctt acacggaggc atcatgacca aacaggaaaa
     2521 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
     2581 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
     2641 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     2701 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     2761 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     2821 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     2881 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     2941 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3001 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3061 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3121 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3181 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3241 cgcttctcct gttccgaccc tgccgcttta ccggaatacc tgtccgcctt tctcccttcg
     3301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3541 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     4201 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4261 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4321 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4381 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4441 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4501 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4561 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4621 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4681 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4741 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4801 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4861 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4921 ggcgtatcac gaggcccttt cgtcttcgaa taaatacctg tgacggaaga tcacttcgca
     4981 gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga
     5041 aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca
     5101 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
     5161 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     5221 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     5281 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     5341 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     5401 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     5461 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     5521 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     5581 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     5641 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     5701 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     5761 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt
     5821 taaggcagtt attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag
     5881 cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca gggcagggtc
     5941 gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc ggaagcagtg
     6001 tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca ggctctcccc gtggaggtaa
     6061 taattgacga tatgatccag ccgaccaggc tttccacgcc cgcgtgccgc tccatgtcgt
     6121 tcgcgcggtt ctcggaaacg cgctgccgcg tttcgtgatt gtcacgctca agcccgtagt
     6181 cccgttcgag cgtcgcgcag aggtcagcga gggcgcggta ggcccgatac ggctcatgga
     6241 tggtgtttcg ggtcgggtga atcttgttga tggcgatatg gatgtgcagg ttgtcggtgt
     6301 cgtgatgcac ggcactgacg cgctgatgct cggcgaagcc aagcccagcg cagatgcggt
     6361 cctcaatcgc gcgcaacgtc tccgcgtcgg gcttctctcc cgcgcggaag ctaaccagca
     6421 ggtgataggt cttgtcggcc tcggaacggg tgttgccgtg ctgggtcgcc atcacctcgg
     6481 ccatgacagc gggcagggtg tttgcctcgc agttcgtgac gcgcacgtga cccaagcgct
     6541 cggtcttgcc ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga
     6601 tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa
     6661 aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc
     6721 tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg
     6781 cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg
     6841 atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg
     6901 ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc
     6961 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt tctggttggc
     7021 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
     7081 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
     7141 cacccgctcg cgggtgggcc tacttcacct atgctgcccg gctgacgccg ttggatacac
     7201 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
     7261 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgctgc
     7321 ttccctgctg ttttgtggaa tatctaccga ctggaaacag gcaaatgcag gaaattactg
     7381 aactgagggg acaggcgaga gacgatgcca aagagctaca ccgacgagct ggccgagtgg
     7441 gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc gttcctggcg
     7501 gtgagggcgg atgtcgaggc ggcgttagcg tccggctatg cgctcgtcac catttgggag
     7561 cacatgcggg aaacggggaa ggtcaagttc tcctacgaga cgttccgctc gcacgccagg
     7621 cggcacatca aggccaagcc cgccgatgtg cccgcaccgc aggccaaggc tgcggaaccc
     7681 gcgccggcac ccaagacgcc ggagccacgg cggccgaagc aggggggcaa ggctgaaaag
     7741 ccggcccccg ctgcggcccc gaccggcttc accttcaacc caacaccgga caaaaaggat
     7801 ctactgtaat ggcgaaaatt cacatggttt
//
