LOCUS       V003013                 8700 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003013
VERSION     V003013
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
   PUBMED   21366919
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8700)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2011"; volume: "4"; issue: "1"; pages: "44"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Crop Improvement and Utilization Research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    complement(137..184)
                     /label="FRT (mutant)"
                     /note="FRT site with a mutation in the core sequence (Azam
                     et al., 1997)"
     terminator      complement(209..460)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(493..1329)
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     transit_peptide complement(1330..1500)
                     /gene="Sul1"
                     /note="Pea rbcS ribulose-bisphosphate carboxylase small
                     subunit transit peptide for plastid targeting"
     promoter        complement(1554..1733)
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     intron          1931..2120
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     CDS             2115..3938
                     /label="GUSPlus(TM)"
                     /note="beta-glucuronidase"
     CDS             3945..3962
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3994..4246
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4268..4292
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    4473..4497
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4711..5299
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5542..6687)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(6989..7780)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(7992..8623)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactggg gtggttttaa ccgtacgacc ggtggccaaa aaggccaaga
      121 gcgcccaata cgcaaagaag ttcctatact tttcagagaa taggaacttc ggaataggaa
      181 cttccctaga tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat
      241 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
      301 gggactctaa tcaaaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
      361 catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat
      421 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagaac
      481 taggccaagc taggcatgat ctaaccctcg gtctctggcg tcgcgactgc gaaatttcgc
      541 gagggtttcc gagatggtga ttgcgcttcg caggtctcca ggcgcgtggg tgcggacgta
      601 gtcagcgcca ttgccgatcg cgtgaagttc cgccgcaagg ctcgctggac ccagatcctt
      661 tacaggaagg ccaacggtgg cgcccaagaa ggatttccgc gacaccgaga ccaatagcgg
      721 aagccccaac gccgacttca gcttttgaag gttcgacagc acgtgcagcg atgtttccgg
      781 tgcggggctc aagaaaaatc ccatccccgg atcgaggatg agccggtcgg cagcgacccc
      841 gctccgtcgc aaggcggaaa cccgcgcctc gaagaaccgc acaatctcgt cgagcgcgtc
      901 ttcgggtcga aggtgaccgg tgcgggtggc gatgccatcc cgctgcgctg agtgcataac
      961 caccagccta cagtccgcct cagcaatatc gggatagagc gcagggtcag gaaatccttg
     1021 gatgtcgttc aggtagccca cgccgcgctt gagcgcatag cgctgggttt ccggttggaa
     1081 gctgtcgatt gaaacacggt gcatctgatc ggacagggcg tctaagagcg gcgcaatacg
     1141 tctgatctca tcggccggcg atactggcct cgcgtccgga tggctggcgg ccggtccgac
     1201 atccacgacg tctgatccga ctcgcagcat ttcgatcgca gccgtgacag cgccggcggg
     1261 gtctagccgc cggctctcat cgaagaagga gtcctcggtg agattcagaa tgccgaacac
     1321 cgtcaccatg cactttactc ttccaccatt gcttgtaatg gaagtaatgt cagtgttgac
     1381 cttcttaact gggaatccag tcatggattt gaggccgccg aatggagcca cagcggccga
     1441 ttgcaccgta gaagcacggc tgactgtagt cacagctgaa gaagaaatca tagaagccat
     1501 ttttctcact tctgtatgaa ttgcaaagct agttgagagt gaatatgaga ctctaattgg
     1561 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
     1621 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
     1681 ctcattaaac tccagaaacc cgcggctcag tggctccttc aacgttgcgg ttctgtcagt
     1741 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
     1801 ttctccgctc atgatcgata tcactagtaa aaggtaccga gctcctgcag tatcgatgcg
     1861 gccgcaaagt cgacgaattc tcccgggtgg atccaaggcc ttctagagga tccccatggt
     1921 agatctgagg gtaaatttct agtttttctc cttcattttc ttggttagga cccttttctc
     1981 tttttatttt tttgagcttt gatctttctt taaactgatc tattttttaa ttgattggtt
     2041 atggtgtaaa tattacatag ctttaactga taatctgatt actttatttc gtgtgtctat
     2101 gatgatgatg atagttacag aaccgacgaa ctagtctgta cccgatcaac accgagaccc
     2161 gtggcgtctt cgacctcaat ggcgtctgga acttcaagct ggactacggg aaaggactgg
     2221 aagagaagtg gtacgaaagc aagctgaccg acactattag tatggccgtc ccaagcagtt
     2281 acaatgacat tggcgtgacc aaggaaatcc gcaaccatat cggatatgtc tggtacgaac
     2341 gtgagttcac ggtgccggcc tatctgaagg atcagcgtat cgtgctccgc ttcggctctg
     2401 caactcacaa agcaattgtc tatgtcaatg gtgagctggt cgtggagcac aagggcggat
     2461 tcctgccatt cgaagcggaa atcaacaact cgctgcgtga tggcatgaat cgcgtcaccg
     2521 tcgccgtgga caacatcctc gacgatagca ccctcccggt ggggctgtac agcgagcgcc
     2581 acgaagaggg cctcggaaaa gtcattcgta acaagccgaa cttcgacttc ttcaactatg
     2641 caggcctgca ccgtccggtg aaaatctaca cgaccccgtt tacgtacgtc gaggacatct
     2701 cggttgtgac cgacttcaat ggcccaaccg ggactgtgac ctatacggtg gactttcaag
     2761 gcaaagccga gaccgtgaaa gtgtcggtcg tggatgagga aggcaaagtg gtcgcaagca
     2821 ccgagggcct gagcggtaac gtggagattc cgaatgtcat cctctgggaa ccactgaaca
     2881 cgtatctcta ccagatcaaa gtggaactgg tgaacgacgg actgaccatc gatgtctatg
     2941 aagagccgtt cggcgtgcgg accgtggaag tcaacgacgg caagttcctc atcaacaaca
     3001 aaccgttcta cttcaagggc tttggcaaac atgaggacac tcctatcaac ggccgtggct
     3061 ttaacgaagc gagcaatgtg atggatttca atatcctcaa atggatcggc gccaacagct
     3121 tccggaccgc acactatccg tactctgaag agttgatgcg tcttgcggat cgcgagggtc
     3181 tggtcgtgat cgacgagact ccggcagttg gcgtgcacct caacttcatg gccaccacgg
     3241 gactcggcga aggcagcgag cgcgtcagta cctgggagaa gattcggacg tttgagcacc
     3301 atcaagacgt tctccgtgaa ctggtgtctc gtgacaagaa ccatccaagc gtcgtgatgt
     3361 ggagcatcgc caacgaggcg gcgactgagg aagagggcgc gtacgagtac ttcaagccgt
     3421 tggtggagct gaccaaggaa ctcgacccac agaagcgtcc ggtcacgatc gtgctgtttg
     3481 tgatggctac cccggagacg gacaaagtcg ccgaactgat tgacgtcatc gcgctcaatc
     3541 gctataacgg atggtacttc gatggcggtg atctcgaagc ggccaaagtc catctccgcc
     3601 aggaatttca cgcgtggaac aagcgttgcc caggaaagcc gatcatgatc actgagtacg
     3661 gcgcagacac cgttgcgggc tttcacgaca ttgatccagt gatgttcacc gaggaatatc
     3721 aagtcgagta ctaccaggcg aaccacgtcg tgttcgatga gtttgagaac ttcgtgggtg
     3781 agcaagcgtg gaacttcgcg gacttcgcga cctctcaggg cgtgatgcgc gtccaaggaa
     3841 acaagaaggg cgtgttcact cgtgaccgca agccgaagct cgccgcgcac gtctttcgcg
     3901 agcgctggac caacattcca gatttcggct acaagaacgc tagccatcac catcaccatc
     3961 acgtgtgaat tggtgaccag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     4021 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     4081 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     4141 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     4201 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attaaactat
     4261 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     4321 acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcaa
     4381 gctttccgcg gggcgcccgt tttacaacgt cgtgactggg agatccacta gcagattgtc
     4441 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     4501 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     4561 gttcgtccat ttgtatgtgc atgccaacca caggagatcc aaaaggatct aggtgaagat
     4621 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4681 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4741 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4801 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4861 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4921 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4981 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5041 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5101 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5161 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5221 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5281 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5341 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5401 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5461 agtgagcgag gaagcggaag aggatctcag taaagcgctg gctgctgaac ccccagccgg
     5521 aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg
     5581 ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt
     5641 cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta
     5701 cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg
     5761 gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc
     5821 gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg
     5881 cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc
     5941 ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc
     6001 caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt
     6061 cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc
     6121 ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc
     6181 gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat
     6241 cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat
     6301 ctgctgcttc gtgtgtttca gcaacgcggc ttgcttggcc tcgctgacct gttttgccag
     6361 gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat
     6421 cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga
     6481 tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc
     6541 ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg
     6601 gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta
     6661 tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc
     6721 cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc
     6781 caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt
     6841 attccgaatc ttgccctgca cgaataccag cgaccccttg cccaaatact tgccgtgggc
     6901 ctcggcctga gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc
     6961 ggcatcgttg cgccacatct aggtactaaa acaattcatc cagtaaaata taatatttta
     7021 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     7081 ccccaatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
     7141 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
     7201 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
     7261 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
     7321 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
     7381 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
     7441 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
     7501 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
     7561 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
     7621 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
     7681 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
     7741 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
     7801 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
     7861 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
     7921 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccacaattat gggtgatgct
     7981 gccaacgcga gagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg
     8041 cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa
     8101 agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct
     8161 ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc
     8221 actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa
     8281 gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc
     8341 gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa
     8401 tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac
     8461 gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg
     8521 gctgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac
     8581 ggccgccagc ccagcggcga gggcaaccag cccggtgagc gtctagtgga ctgatgggct
     8641 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
//
