LOCUS       40924_41435        9108 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter shuttle vector pSUN202, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Argueta C, Yuksek K, Summers M.
  TITLE     Construction and use of GFP reporter vectors for analysis of 
            cell-type-specific gene expression in Nostoc punctiforme
  JOURNAL   J. Microbiol. Methods 59 (2), 181-188 (2004)
  PUBMED    15369854
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Argueta C, Yuksek K, Summers ML.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St.,
            Northridge, CA 91330-8303, USA
REFERENCE   3  (bases 1 to 9108)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2004"; volume: "59"; issue: "2"; pages: 
            "181-188"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St., 
            Northridge, CA 91330-8303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(215..928)
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    985..1027
                     /label=multiple cloning site
                     /note="multiple cloning site"
     terminator      complement(1209..1256)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1305..1321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1329..1345)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1353..1383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1398..1419)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1707..2295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2469..3326)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3344..9108
                     /label=pDC1 cyanobacterial origin of replication
                     /note="pDC1 cyanobacterial origin of replication"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgtatttgta gagctcatcc atgccatgtg
      241 taatcccagc agcagttaca aactcaagaa ggaccatgtg gtcacgcttt tcgttgggat
      301 ctttcgaaag ggcagattgt gtcgacaggt aatggttgtc tggtaaaagg acagggccat
      361 cgccaattgg agtattttgt tgataatggt ctgctagttg aacggatcca tcttcaatgt
      421 tgtggcgaat tttgaagtta gctttgattc cattcttttg tttgtctgcc gtgatgtata
      481 cattgtgtga gttatagttg tactcgagtt tgtgtccgag aatgtttcca tcttctttaa
      541 aatcaatacc ttttaactcg atacgattaa caagggtatc accttcaaac ttgacttcag
      601 cacgcgtctt gtagttcccg tcatctttga aagatatagt gcgttcctgt acataacctt
      661 cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atctgggtat cttgaaaagc
      721 attgaacacc ataagagaaa gtagtgacaa gtgttggcca tggaacaggt agttttccag
      781 tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca ccctctccac
      841 tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt gggacaactc
      901 cagtgaaaag ttcttctcct ttactcatta tgagtctcca gtttgtttca gttaaaacgt
      961 agtagtgttg gtaaattact cgaggaattc ggtaccccgg gttcgaaatc gataagcttg
     1021 gctgcaggtc gactctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg
     1081 gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca
     1141 ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatatccgga tatagttcct
     1201 cctttcagca aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat
     1261 tgctcagcgg tggcagcagc gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     1321 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1381 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1441 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1501 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1561 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1621 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1681 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1741 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1801 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1861 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     1921 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1981 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2041 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2101 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2161 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2221 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2281 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2341 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2401 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2461 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2521 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2581 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2641 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2701 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2761 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2821 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2881 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2941 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3001 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3061 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3121 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3181 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3241 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3301 gcgacacgga aatgttgaat actcatactc ttcctttttc aatcgcgtct cgtgccgatt
     3361 agtatactta ttgccgatgc atttaccaaa atttgcaacc ggagaagttc acttgtccag
     3421 taaaaacttc caaaaattta ccggacaacc ttatgttatc cgccagcaaa ttttgacgca
     3481 cagtacgcca gttttggctg aaagtttcaa ctgttgcgaa aagtgcctag tgaactcgtt
     3541 gtacaaaaaa attttcgcgt ttggcatcaa gccacgcact cttaaggttt tgacaaaata
     3601 tgtcattcca agcttctgca atggaattta aagttaatcc ccatccgcga tatttcggca
     3661 ctagggaagc agcatgatcc atgcattggc gcagactgcc agagcatagc tcatcacatg
     3721 tccagactac ctggttttct tcatccaact catgttctac cagcatcttc aaaacccaca
     3781 tcttaccccc tcaaaaaaat tctcaaaaat gtcccgtcac ttcctggggc ttgtgatgat
     3841 tgctttgcaa tcgtctgcct gcgggacggc tcgattttcg tgaaactggc actgtgccag
     3901 tttacaacgc cacttccgcc gctttgcgcg catggttgtt agcttgaatt gctcgcggta
     3961 aatgttgcag cgagccatcc aggttttacc cttcatttgg aagaaacgcc cagacagttc
     4021 cccgctggca gctggtaagt gttcggctgc tgctttctgg ctcctggcag gatgccatca
     4081 gaaaacgcga tcgccccttc acccaatgac tgatacccaa tgactgatac ccaatgtctg
     4141 actgagtgcc ctcgctactc caagcccagc actcagacgc ttcattgctt acagcccggt
     4201 caagttgagc gtattcatac ttcgcttggc acagttgcct tttctccatg ccgagcgttc
     4261 gctgatgccg ccgcactgtg cgcgtgtgga tgcccaattt acagctgacc gcattttgag
     4321 aagtcccgta atgaatgaag cttttagaaa cgaacacccg cgacggactg acatgaagaa
     4381 caaaaggtac ttcccccgct cccaatttaa acgaggattg cccctcatcc cccaacagtt
     4441 cgtttggatg tggcgctccg taagacttgc gatactccgg tttcagtttg cggttagccg
     4501 cgtatctact tctttgttgc aaacgctgtg tgactatgcc cgtggtcagc gctcgaatgt
     4561 tggcgttgac ctcccataac ggacattctg ctactacccc ccaatctttt aaatttaaat
     4621 gccaagccac cttagtcagt ccgcccaggt aaaccgtgag cagtccaatt cttaccttgt
     4681 atcccgacaa aaatgcccct actgcttttc cctggtcaag ccatctgtac acagtggctt
     4741 gactaactcc caatatctcc tctaccagca cgagcggaat ttctttaacc cgtccgctcc
     4801 catcatcaaa accgcgcaac aaataccaca gccgcgccca aggttgacgg ctaattccac
     4861 tgtgtaaccg caccatgaac ggctgcaaaa ttatctcggc tttgactagt atcaaaacca
     4921 acgggagtgg tacatttgaa cttaaggctg aacgatcctc ctgtgaagtc agggggcaat
     4981 ggttcagccc ctcaacttgg gtagctgcgc gattttgatt ggccactggt tttcactcaa
     5041 acctggttca atgtgccaat cgcttcacta aatttaagtt ttcgcttccg ctcgactgaa
     5101 tactgtgctg atcctcgaag ggtggtaata tgtttgaagt tcattgcttc tgggtgatta
     5161 aggaatgatt gcccagtagt ttttccaaat cttcacccta tctctacact gtttaccgat
     5221 ccacgcagtt gtataccaac cccaaaactt gataaattca agttaacttt tgttattgtc
     5281 tggtttttgg attacggact tgatgggcct ggtgaacgac tgctgcatgg aactctgtaa
     5341 acatgtgtag acgactgggt aatcaatttc tccactaccg gactttttct tatataagtt
     5401 ttcttgtgta agatttgcac ttttagaagt atgcgtatcc tattaggaaa actcacagtt
     5461 ttaagaaaaa ctgtatttag catacatcgg ttagagccgt gtatcaattg acaacatcct
     5521 atacaggatc taggttttgc acgtgccata aggtgacgaa atatttattt cgtcacataa
     5581 agcttgaaca gtaatcactg caagcagttg aaattctacc cgtgtagtat gggtatagaa
     5641 ttttcctttt tctgtttttt tgtagtcaaa atttaggcaa aacatgctac tcatttgcta
     5701 ctcaaaatat gtttgaaacc attgattaat cgtttatgcc aacatttcac cccaaaatac
     5761 acgaaatctt gtaactattg ctatgactag ctttcacccg ttggtgtaat gattctgtag
     5821 aatgttgtac ccccttactg gtaaaccctt gctgtgacta gctttcatca atgaccaatg
     5881 gatttcaagt ccgcgtgtcc ttagttatcc ccgctacatc tatctgccat tctgcttttc
     5941 aagcattaaa agcattgttt ccaattcttc ccaatgcggt tgtgaaagag tttggtagca
     6001 ctgaagcaac agttcggaat gctccggcgc atcatcagaa gctatgctta acagcttgtg
     6061 gtattgttta agctggttaa attcatccaa ggcacgggta atcaaaacct ttgcagattc
     6121 gtttctagtc tgtgacatca tgttttgcat ccccgattac attggttcta cttggcactc
     6181 atcgacccat tccacttcac ggtccgtcta cttcaaacca gggatgctct tctggcaagg
     6241 acaccgcata ttgccaaaaa gcaccagtcc aaaaaagccc aatcacctca acaggatgca
     6301 gaatttcccc cctggggact gtcatcctat gcagcacggt ttgcccgata tgatatccag
     6361 gtttactaaa agcctttgta atccgcaaat gccaaaaact cagcgcgttg catcgcagca
     6421 gcccgaaacg ggcgcgttta acgctaccat tgcttattgc ctccttagtg ggctaacaac
     6481 ttttaagggg gaagtcgtgc cggtggggga gatgtggtaa tttctcctgc cggggcgcat
     6541 ttaaggcctg acttcatttg tcgcatcagg tatcttgaat tattgaacca gatttgagcg
     6601 caaaaagcga tcgcgctgcg gggcgattac tgcatgttct agcttgagca gtatttattc
     6661 tgctagaact cttgagctac tttattcggg acgggagatc gtttaaccat cctatgtggg
     6721 tagtgatttt caagggagcg agacgacgct acgatttact cgctacaacc ttgtttacta
     6781 tcgtctactt tttgtctaca aacagtctaa ggtagtaaat catgtattgt caacattgtc
     6841 tacaattttg tggacaatct ctctaggtta acctagtatg tagactaggt gtatacaaat
     6901 agtctacaaa tgcgtaacaa tataatttca tttcgcttta gtgacgctga attagaagcg
     6961 ttgcaagcat tccaaactcc agaagataaa tcccctagcc aaatagccgc aagattagta
     7021 aggggaataa ttgcagggat ggtagaaccg tctacaccgt ctacacctgt agacatacag
     7081 gaattagtta agcagcaagt cgagcaggct atcaccgact cgcaactgtt gaaagatatg
     7141 attgcgctca atacctctca cctaacaaaa ggcatcgaca aaattaagca ggaggtcgat
     7201 gaacgtttgg aggaactgcg gggaaaatcg aaagcccggt agcagagaag gatactagcg
     7261 ctaccgggca agtaatgaat aatagcgact taatcttagt ccgtgatcgc gtccttgcca
     7321 agctgaaaat gggtaagcaa tcaaccgccg ggaaggcaat cgacgcattt atcaaagaac
     7381 tgcacaaaac tccgaccgca ccggaggaac aaaagccgtc tacctgggaa gcccgcgctg
     7441 acaacttcct caaggaggtt gcaggttgca ggcaatggct aaccaccgaa tcaaaatgcg
     7501 cctcatgggt actaaggaag atttggagcg ctggctgtgg tttgtgggca agatgcaaga
     7561 gcggggactg gcaagcatca ttgagaaaag ttcgccttac aaaaacagag gcgaatcgct
     7621 gcaacaccgc gtctacttgg aagttgactt gctgttggat gccccagaag atgagattaa
     7681 gcctttataa ccccagtaca gtgccacaca tggacggttt caacctgtct acagtccgtc
     7741 tacaccacaa caacacacga cgatcggtgc ggttacagtg caacaataga cggtgacaag
     7801 tgcggttgca gtgcaacaat agacggtgac aagtgcggtt gcagtgcaac aatagagcgt
     7861 ctacacccaa accagttaaa aaattgcctg tagacggcta aaaaccgctt ttgaagagac
     7921 tttcagcaac tgtagacaaa tttttggggg agcattttta acagaaatac cccaaaagtt
     7981 agccattttt cttaacagaa tgctgatttt tgtagactcg ttcggcttaa caaaatgctc
     8041 aaaaacatag cagccgggtg cgaacgtggt tgcaattctc caatgttcta gctttaaata
     8101 tcagaatgta actagaactc tgtggaacca tgcccaaaaa gagatgcgga ctcggctttg
     8161 actgcgggtc gatgatgtta caacctggga tagaccccgg cgactgcgaa aattacgcca
     8221 cctgtggagg agccacccaa ctcaccccag aagaagagtt tgaattgata cgttggcggg
     8281 aaattcaaaa ccgggaaagg caagaggagt gggagcgcca gcgcgaagca attcgggtca
     8341 accgccacca agctgcattg atgatgctga tgggtcgcgc ttgcccccaa acgcccgaat
     8401 cattgggtgt tgtggatttg ttagcccaag ttgcaacctt agttgaggaa gtcagatcgc
     8461 aactgcccca gtttgaaggg cagtacatcg cacctgaaaa gtgcgaagcc cacacttaca
     8521 acgtcaaacg acccaaaggt gtatattgct acaataagct gaccgctgcc gaggcaattt
     8581 ttgagccgag cgagaaaagc gataaagtcc gagtcatcca cctcagccac gacggcgacc
     8641 cccgcgactc agaggcgcgg ctgggcatcg aacgccgaaa cgccttgact caagccagga
     8701 ctcagatcaa cgttgcccaa gttgcactcg ctcaagcctt ggagttgctg caatgttcta
     8761 gcagaataaa taatcagcaa gctagaacac tcgcgcactc gcccccatcc agtgacgaaa
     8821 caccgtgaaa ggcgatcgcc ccaggtgcat caaaatttgc aaagtgtttt attgcaaatc
     8881 ttgatgcagc cacgcactgc atcactcacc agcttccagc ttccgacttc cacttacttc
     8941 aactagctac tctaactgga agttgaagtt gtaagttacc ttgcgggtct aattttccta
     9001 ttctgattcg ctagattctt cttttagact ttcatcgtat gtttgataaa cctggatgca
     9061 acgtcctggt ttacgctcaa agctttccgc cgatataaca aaagaatt
//
