LOCUS       V003016                 6263 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003016
VERSION     V003016
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
   PUBMED   21366919
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 6263)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6263)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2011"; volume: "4"; issue: "1"; pages: "44"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Crop Improvement and Utilization Research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    complement(137..184)
                     /label="FRT (mutant)"
                     /note="FRT site with a mutation in the core sequence (Azam
                     et al., 1997)"
     terminator      complement(209..460)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(493..1329)
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     transit_peptide complement(1330..1500)
                     /gene="Sul1"
                     /note="pea rbcS ribulose-bisphosphate carboxylase small
                     subunit transit peptide for plastid targeting"
     promoter        complement(1554..1733)
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     misc_feature    2036..2060
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      2274..2862
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3105..4250)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(4552..5343)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(5555..6186)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactggg gtggttttaa ccgtacgacc ggtggccaaa aaggccaaga
      121 gcgcccaata cgcaaagaag ttcctatact tttcagagaa taggaacttc ggaataggaa
      181 cttccctaga tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat
      241 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
      301 gggactctaa tcaaaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
      361 catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat
      421 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagaac
      481 taggccaagc taggcatgat ctaaccctcg gtctctggcg tcgcgactgc gaaatttcgc
      541 gagggtttcc gagatggtga ttgcgcttcg caggtctcca ggcgcgtggg tgcggacgta
      601 gtcagcgcca ttgccgatcg cgtgaagttc cgccgcaagg ctcgctggac ccagatcctt
      661 tacaggaagg ccaacggtgg cgcccaagaa ggatttccgc gacaccgaga ccaatagcgg
      721 aagccccaac gccgacttca gcttttgaag gttcgacagc acgtgcagcg atgtttccgg
      781 tgcggggctc aagaaaaatc ccatccccgg atcgaggatg agccggtcgg cagcgacccc
      841 gctccgtcgc aaggcggaaa cccgcgcctc gaagaaccgc acaatctcgt cgagcgcgtc
      901 ttcgggtcga aggtgaccgg tgcgggtggc gatgccatcc cgctgcgctg agtgcataac
      961 caccagccta cagtccgcct cagcaatatc gggatagagc gcagggtcag gaaatccttg
     1021 gatgtcgttc aggtagccca cgccgcgctt gagcgcatag cgctgggttt ccggttggaa
     1081 gctgtcgatt gaaacacggt gcatctgatc ggacagggcg tctaagagcg gcgcaatacg
     1141 tctgatctca tcggccggcg atactggcct cgcgtccgga tggctggcgg ccggtccgac
     1201 atccacgacg tctgatccga ctcgcagcat ttcgatcgca gccgtgacag cgccggcggg
     1261 gtctagccgc cggctctcat cgaagaagga gtcctcggtg agattcagaa tgccgaacac
     1321 cgtcaccatg cactttactc ttccaccatt gcttgtaatg gaagtaatgt cagtgttgac
     1381 cttcttaact gggaatccag tcatggattt gaggccgccg aatggagcca cagcggccga
     1441 ttgcaccgta gaagcacggc tgactgtagt cacagctgaa gaagaaatca tagaagccat
     1501 ttttctcact tctgtatgaa ttgcaaagct agttgagagt gaatatgaga ctctaattgg
     1561 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
     1621 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
     1681 ctcattaaac tccagaaacc cgcggctcag tggctccttc aacgttgcgg ttctgtcagt
     1741 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
     1801 ttctccgctc atgatcgata tcactagtaa aaggtaccga gctcctgcag tatcgatgcg
     1861 gccgcaaagt cgacgaattc tcccgggtgg atccaaggcc ttctagaaaa actcgagaac
     1921 gttgggcccc aattggctag caagctttcc gcggggcgcc cgttttacaa cgtcgtgact
     1981 gggagatcca ctagcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     2041 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     2101 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacaggaga
     2161 tccaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2221 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2281 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2341 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2401 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2461 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2521 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2581 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2641 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2701 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2761 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2821 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2881 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2941 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3001 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagaggatct cagtaaagcg
     3061 ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc
     3121 aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct
     3181 tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg
     3241 aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt
     3301 cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca
     3361 gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca
     3421 cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg
     3481 gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa
     3541 taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc
     3601 ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca
     3661 tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa
     3721 atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca
     3781 gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac
     3841 aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcttgcttg
     3901 gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata
     3961 gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga
     4021 cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg
     4081 tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg
     4141 cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc
     4201 gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct
     4261 tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct
     4321 ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc
     4381 tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc
     4441 ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag
     4501 tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc
     4561 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
     4621 aaatagctcg acatactgtt cttccccaat atcctccctg atcgaccgga cgcagaaggc
     4681 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
     4741 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
     4801 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
     4861 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
     4921 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
     4981 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
     5041 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
     5101 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
     5161 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
     5221 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
     5281 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
     5341 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
     5401 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
     5461 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
     5521 aaaccacaat tatgggtgat gctgccaacg cgagagcggg ccgggagggt tcgagaaggg
     5581 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca
     5641 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac
     5701 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat
     5761 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat
     5821 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc
     5881 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc
     5941 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt
     6001 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc
     6061 gcgaggtatc cacaacgccg gcggctgcgg tgtctcgcac acggcttcga cggcgtttct
     6121 ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg
     6181 agcgtctagt ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6241 tcggggagct gttggctggc tgg
//
