LOCUS       40924_41405       11715 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSUC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Obermaier S, Fuertges L.
  TITLE     Biosynthesis of dimeric naphthopyrones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   Obermaier S, Fuertges L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2017) Pharmaceutical Chemistry, University 
            Freiburg, Fahnenbergplatz, Freiburg 79085, Germany
REFERENCE   3  (bases 1 to 11715)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11715)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2017) Pharmaceutical Chemistry, University Freiburg, 
            Fahnenbergplatz, Freiburg 79085, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      4..292
                     /regulatory_class="terminator"
     CDS             715..1737
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     protein_bind    complement(1812..1845)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     primer_bind     complement(2315..2331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2601..3189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3316..4173)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4174..4265)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    4558..4591
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      complement(4905..10161)
                     /direction=LEFT
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans
                     (Aleksenko and Clutterbuck, 1996)"
     regulatory      10437..11702
                     /regulatory_class="promoter"
ORIGIN
        1 caaggatcca ctagtacagc agaagaatct ctctccgctg ttgcttcagt gtctgccatg
       61 cattaacttc atcctactgt cctacccgca gtacccattc acatttgcgg cagataccag
      121 gttgttttct attccctcgg ttcttcagtt cttcagatat tacattactc gagaaattgg
      181 cggcgatgca ggagtttggt tggttttaat tgtttcagtc tccttgacct gatttgtatg
      241 acatgcaatg tttcgacgga agactatctc ggtgaatata cgtcatgaat cattcgaaca
      301 atgcgcgatt caagacatta aaaatggaaa atttcaaaaa attgcgcccg cagcgcgtgc
      361 atacaaaatt catcccaata caagtgtaat gctagtggag gtcaacacat caatgcctat
      421 tttggtttag tcgtccaggc ggtgagcaca aaatttgtgt cgtttgacaa gatggttcat
      481 ttaggcaact ggtcagatca gccccacttg tagcagtagc ggcggcgctc gaagtgtgac
      541 tcttattagc agacaggaac gaggacatta ttatcatctg ctgcttggtg cacgataact
      601 tggtgcgttt gtcaagcaag gtaagtggac gacccggtca taccttctta agttcgccct
      661 tcctcccttt atttcagatt caatctgact tacctattct acccaagcat ccaaatgaaa
      721 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
      781 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
      841 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
      901 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggag
      961 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     1021 ctgcctgaaa ccgaactgcc cgctgttctg gagccggtcg cggaggccat ggatgcgatt
     1081 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     1141 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     1201 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     1261 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     1321 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     1381 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     1441 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcgcctc
     1501 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     1561 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     1621 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     1681 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     1741 agtagatgcc gaccgatccc cactaccgca ttaagacctc agcgcggccg caaatttaaa
     1801 taaaatgaag tgaagttcct atactttcta gagaatagga acttctatag tgagtcgaat
     1861 aagggcgaca caaaatttat tctaaatgca taataaatac tgataacatc ttatagtttg
     1921 tattatattt tgtattatcg ttgacatgta taattttgat atcaaaaact gattttccct
     1981 ttattatttt cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata
     2041 caaaaaatca taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc
     2101 aacgtatctt atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat
     2161 atatcaagca aagtgacagg cgcccttaaa tattctgaca aatgctcttt ccctaaactc
     2221 cccccataaa aaaacccgcc gaagcgggtt tttacgttat ttgcggatta acgattactc
     2281 gttatcagaa ccgcccaggg ggcccgagct taagactggc cgtcgtttta caacacagaa
     2341 agagtttgta gaaacgcaaa aaggccatcc gtcaggggcc ttctgcttag tttgatgcct
     2401 ggcagttccc tactctcgcc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2461 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2521 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2941 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3001 gagttcttga agtggtgggc taactacggc tacactagaa gaacagtatt tggtatctgc
     3061 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg
     3241 cgcgtaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt
     3301 aatgctctgc ttttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3361 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3421 accatctggc cccagcgctg cgatgatacc gcgagaacca cgctcaccgg ctccggattt
     3481 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3541 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3601 tagtttgcgc aacgttgttg ccatcgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3661 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3721 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3781 agtgttatca ctcatggtta tggcagcgct acataattct cttactgtca tgccatccgt
     3841 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3901 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3961 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4021 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4081 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4141 aataagggcg acacggaaat gttgaatact catattcttc ctttttcaat attattgaag
     4201 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4261 acaaataggg gtcagtgtta caaccaatta accaattctg aacattatcg cgagcccatt
     4321 tatacctgaa tatggctcat aacacccctt gtttgcctgg cggcagtagc gcggtggtcc
     4381 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtgggga
     4441 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     4501 gactgggcct ttcgcccggg ctaattatgg ggtgtcgccc ttattcgact ctatagtgaa
     4561 gttcctattc tctagaaagt ataggaactt ctgaagtggg gatttaaatg cggccgcgct
     4621 gagggtttaa tcgacgaagc agctgacggc cagtgccaag cttaacgcgt acccgggccc
     4681 agtatatgtt ccgcagatga ctggagctct gccatacgtg ccctctcaag caccatttgt
     4741 tccatctaca gagactagtc accaactagt ctatcaagac tcacagggta cattgctgag
     4801 accaactgac cagaggcagg gtagcggatt gacggctcca tctccttcac ttacaaggtc
     4861 tattgaaagc cctttagcat caccaagcgg agaatagatt gttaagctta ttttttgtat
     4921 actgttttgt gatagcacga agtttttcca cggtatcttg taaaaatata tatttgtggc
     4981 gggcttacct acatcaaatt aataagagac taattataaa ctaaacacac aagcaagcta
     5041 ctttagggta aaagtttata aatgcttttg acgtataaac gttgcttgta tttattatta
     5101 caattaaagg tggatagaaa acctagagac tagttagaaa ctaatctcag gtttgcgtta
     5161 aactaaatca gagcccgaga ggttaacaga acctagaagg ggactagata tccgggtagg
     5221 gaaacaaaaa aaaaaaacaa gacagccaca tattagggag actagttaga agctagttcc
     5281 aggactagga aaataaaaga caatgatacc acagtctagt tgacaactag atagattcta
     5341 gattgaggcc aaagtctctg agatccaggt tagttgcaac taatactagt tagtatctag
     5401 tctcctataa ctctgaagct agaataactt actactatta tcctcaccac tgttcagctg
     5461 cgcaaacgga gtgattgcaa ggtgttcaga gactagttat tgactagtca gtgactagca
     5521 ataactaaca aggtattaac ctaccatgtc tgccatcacc ctgcacttcc tcgggctcag
     5581 cagccttttc ctcctcattt tcatgctcat tttccttgtt taagactgtg actagtcaaa
     5641 gactagtcca gaaccacaaa ggagaaatgt cttaccactt tcttcattgc ttgtctcttt
     5701 tgcattatcc atgtctgcaa ctagttagag tctagttagt gactagtccg acgaggactt
     5761 gcttgtctcc ggattgttgg aggaactctc cagggcctca agatccacaa cagagccttc
     5821 tagaagactg gtcaataact agttggtctt tgtctgagtc tgacttacga ggttgcatac
     5881 tcgctccctt tgcctcgtca atcgatgaga aaaagcgcca aaactcgcaa tatggctttg
     5941 aaccacacgg tgctgagact agttagaatc tagtcccaaa ctagcttgga tagcttacct
     6001 ttgccctttg cgttgcgaca ggtcttgcag ggtatggttc ctttctcacc agctgattta
     6061 gctgccttgc taccctcacg gcggatctgc cataaagagt ggctagaggt tataaattag
     6121 cactgatcct aggtacgggg ctgaatgtaa cttgcctttc ctttctcatc gcgcggcaag
     6181 acaggcttgc tcaaattcct accagtcaca ggggtatgca cggcgtacgg accacttgaa
     6241 ctagtcacag attagttagc aactagtctg cattgaatgg ctgtacttac gggccctcgc
     6301 cattgtcctg atcatttcca gcttcaccct cgttgctgca aagtagttag tgactagtca
     6361 aggactagtt gaaatgggag aagaaactca cgaattctcg actcccttag tattgtggtc
     6421 cttggacttg gtgctgctat atattagcta atacactagt tagactcaca gaaacttacg
     6481 cagctcgctt gcgcttcttg gtaggagtcg gggttgggag aacagtgcct tcaaacaagc
     6541 cttcatacca tgctacttga ctagtcaggg actagtcacc aagtaatcta gataggactt
     6601 gcctttggcc tccatcagtt ccttcatagt gggaggacca ttgtgcaatg taaactccat
     6661 gccgtgggag ttcttgtcct tcaagtgctt gaccaatatg tttctgttgg cagagggaac
     6721 ctgtcaacta gttaataact agtcagaaac tatgatagca gtagactcac tgtacgcttg
     6781 aggcatccct tcactcggca gtagacttca tatggatgga tatcaggcac gccattgtcg
     6841 tcctgtggac tagtcagtaa ctaggcttaa agctagtcgg gtcggcttac tatcttgaaa
     6901 tccggcagcg taagctcccc gtccttaact gcctcgagat agtgacagta ctctggggac
     6961 tttcggagat cgttatcgtt atcgcgaatg ctcggcatac taactgttga ctagtcttgg
     7021 actagtcccg agcaaaaagg attggaggag gaggaggaag gtgagagtga gacaaagagc
     7081 gaaataagag cttcaaaggc tatctctaag cagtatgaag gttaagtatc tagttcttga
     7141 ctagatttaa agagatttcg actagttatg tacctggagt ttggatatag gaatgtgttg
     7201 tggtaacgaa atgtaagggg gaggaaagaa aaagtcgtca agaggtaact ctaagtcggc
     7261 cattcctttt tgggaggcgc taaccataaa cggcatggtc gacttagagt tagctcaggg
     7321 aatttaggga gttatctgcg accaccgagg aacggcggaa tgccaaagaa tcccgatgga
     7381 gctctagctg gcggttgaca accccacctt ttggcgtttc tgcggcgttg caggcgggac
     7441 tggatacttc gtagaaccag aaaggcaagg cagaacgcgc tcagcaagag tgttggaagt
     7501 gatagcatga tgtgccttgt taactaggta ccaatctgca gtatgcttga tgttatccaa
     7561 agtgtgagag aggaaggtcc aaacatacac gattgggaga gggcctaggt ataagagttt
     7621 ttgagtagaa cgcatgtgag cccagccatc tcgaggagat taaacacggg ccggcatttg
     7681 atggctatgt tagtacccca atggaaacgg tgagagtcca gtggtcgcag ataactccct
     7741 aaattccctg agctaactct aagtcgacca tgccgtttat ggttagcgcc tcccaaaaag
     7801 gaatggccga cttagagtta cctcttgacg actttttctt tcctccccct tacatttcgt
     7861 taccacaaca cattcctata tccaaactcc aggtacataa ctagtcgaaa tctctttaaa
     7921 tctagtcaag aactagatac ttaaccttca tactgcttag agatagcctt tgaagctctt
     7981 atttcgctct ttgtctcact ctcaccttcc tcctcctcct ccaatccttt ttgctcggga
     8041 ctagtccaag actagtcaac agttagtatg ccgagcattc gcgataacga taacgatctc
     8101 cgaaagtccc cagagtactg tcactatctc gaggcagtta aggacgggga gcttacgctg
     8161 ccggatttca agatagtaag ccgacccgac tagctttaag cctagttact gactagtcca
     8221 caggacgaca atggcgtgcc tgatatccat ccatatgaag tctactgccg agtgaaggga
     8281 tgcctcaagc gtacagtgag tctactgcta tcatagtttc tgactagtta ttaactagtt
     8341 gacaggttcc ctctgccaac agaaacatat tggtcaagca cttgaaggac aagaactccc
     8401 acggcatgga gtttacattg cacaatggtc ctcccactat gaaggaactg atggaggcca
     8461 aaggcaagtc ctatctagat tacttggtga ctagtccctg actagtcaag tagcatggta
     8521 tgaaggcttg tttgaaggca ctgttctccc aaccccgact cctaccaaga agcgcaagcg
     8581 agctgcgtaa gtttctgtga gtctaactag tgtattagct aatatatagc agcaccaagt
     8641 ccaaggacca caatactaag ggagtcgaga attcgtgagt ttcttctccc atttcaacta
     8701 gtccttgact agtcactaac tactttgcag caacgagggt gaagctggaa atgatcagga
     8761 caatggcgag ggcccgtaag tacagccatt caatgcagac tagttgctaa ctaatctgtg
     8821 actagttcaa gtggtccgta cgccgtgcat acccctgtga ctggtaggaa tttgagcaag
     8881 cctgtcttgc cgcgcgatga gaaaggaaag gcaagttaca ttcagccccg tacctaggat
     8941 cagtgctaat ttataacctc tagccactct ttatggcaga tccgccgtga gggtagcaag
     9001 gcagctaaat cagctggtga gaaaggaacc ataccctgca agacctgtcg caacgcaaag
     9061 ggcaaaggta agctatccaa gctagtttgg gactagattc taactagtct cagcaccgtg
     9121 tggttcaaag ccatattgcg agttttggcg ctttttctca tcgattgacg aggcaaaggg
     9181 agcgagtatg caacctcgta agtcagactc agacaaagac caactagtta ttgaccagtc
     9241 ttctagaagg ctctgttgtg gatcttgagg ccctggagag ttcctccaac aatccggaga
     9301 caagcaagtc ctcgtcggac tagtcactaa ctagactcta actagttgca gacatggata
     9361 atgcaaaaga gacaagcaat gaagaaagtg gtaagacatt tctcctttgt ggttctggac
     9421 tagtctttga ctagtcacag tcttaaacaa ggaaaatgag catgaaaatg aggaggaaaa
     9481 ggctgctgag cccgaggaag tgcagggtga tggcagacat ggtaggttaa taccttgtta
     9541 gttattgcta gtcactgact agtcaataac tagtctctga acaccttgca atcactccgt
     9601 ttgcgcagct gaacagtggt gaggataata gtagtaagtt attctagctt cagagttata
     9661 ggagactaga tactaactag tattagttgc aactaacctg gatctcagag actttggcct
     9721 caatctagaa tctatctagt tgtcaactag actgtggtat cattgtcttt tattttccta
     9781 gtcctggaac tagcttctaa ctagtctccc taatatgtgg ctgtcttgtt tttttttttt
     9841 gtttccctac ccggatatct agtccccttc taggttctgt taacctctcg ggctctgatt
     9901 tagtttaacg caaacctgag attagtttct aactagtctc taggttttct atccaccttt
     9961 aattgtaata ataaatacaa gcaacgttta tacgtcaaaa gcatttataa acttttaccc
    10021 taaagtagct tgcttgtgtg tttagtttat aattagtctc ttattaattt gatgtaggta
    10081 agcccgccac aaatatatat ttttacaaga taccgtggaa aaacttcgtg ctatcacaaa
    10141 acagtataca aaaaataagc ttaacaatct attctccgct tggtgatgct aaagggcttt
    10201 caatagacct tgtaagtgaa ggagatggag ccgtcaatcc gctaccctgc ctctggtcag
    10261 ttggtctcag caatgtaccc tgtgagtctt gatagactag ttggtgacta gtctctgtag
    10321 atggaacaaa tggtgcttga gagggcacgt atggcagagc tccagtcatc tgcggaacat
    10381 atactgggcc cgggaagatc tcatggtcat agctgtttcc gctgagggtt taattaagat
    10441 cacgtggatg gcctgtgcgg ggttggcagg cgtggtatga tagtatcctt ccgctgctca
    10501 ttacctgtca tcgtcggctc agattatgag gcatggcacg attgattcat ttcatttccc
    10561 tggggcactg ctggctgcga ccccgccctt tgcggccaca cttgggtttg atgaaagctg
    10621 cttgtgtcct ggccgtggct aagcggtttg cgcttttcgg tccaggtatc gtactaggta
    10681 cagtccaatc ccggtagtgt gggaagcttc aaaatgatcg ttgagaccac attcccggac
    10741 agatattagg gtcaatgtta agttgatggc catgaagtgc tattgatatg agctaataaa
    10801 atgtcggtgt ctggcgtgtg gtgatatgta tgctaactgt caggcatgtt tcgagccgat
    10861 gcctcttgga agccttgtta tggtataatt ctcagagcat agagcatcat cgaaaggcga
    10921 acgatttcag ctgtgagaac gtagtaggcc gtcccccggg agcccaaatc atgagaaacg
    10981 aaggtctttt ccatctagcc ggcgcagaat gctgcagtga aacgtacttt cattctctgc
    11041 ctactcattg aaatgagcac gccataacgg gctacgaact tatctcttgg cagaataatg
    11101 cttgaatcgc gatcagcgaa gttgccattg agagcatcgt ccttggggat gagacgggcg
    11161 aatacaagag cacgtgttgg caagattctg ctccaattca cctagaatca ttgatttggc
    11221 attctgcaag catcccttca cctgcaggac gcgccgtcac ccgcttctct cttctccgcg
    11281 ggcgttcctg gcctgaccca ttctctggcc ctttcatgtc cgacactcct ccgaggctcc
    11341 gacctactcc tatgggggtc gaatcaaccc acaccaagag ctggcccaga tacagcgtac
    11401 ccaacatgca atttcaattt ccctcacgtc attcacccct ctccgaatcc gatcagggaa
    11461 gatcggcggc ccaagggcct tgtttcggag gaaaaggggg aaacctcggg gattccgcgc
    11521 tgaatctccc ccggaaccct aagcggggcc cctccgcatc tcacatttcc ccgaaaccat
    11581 atcacactcg gtcgatagtc cacggagatt tcccggccca tgcatgacaa ccatgccact
    11641 atttaaccca tgccatccct cccatggcca acgaatggaa gcacccctcc aaggccctcc
    11701 cacgctagct acgta
//
