LOCUS       62056_20235        3166 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(12..824)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(1456..2000)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     2275..2291
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(2295..2351)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2361..2377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2385..2401)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2409..2439)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2454..2475)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
       61 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
      121 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
      181 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
      241 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
      301 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
      361 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
      421 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
      481 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
      541 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
      601 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
      661 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
      721 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
      781 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
      841 ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc
      901 gttccactga gcgtcagacc ccgtagaaaa acgtctcatt ttcgccaaaa gttggcccag
      961 ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc
     1021 acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttact gatttagtgt
     1081 atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc
     1141 agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag
     1201 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     1261 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     1321 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     1381 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     1441 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     1501 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttcccctggc
     1561 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     1621 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     1681 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     1741 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     1801 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     1861 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     1921 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     1981 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt
     2041 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     2101 aattctcatg tttgacagct tatcatcgat aagctcattc gccattcagg ctgcgcaact
     2161 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     2221 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     2281 cgacggccag tgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
     2341 agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     2401 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     2461 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2521 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggaacg
     2581 ccatgagcgg cctcatttct tattctgagt tacaacagtc cgcaccgctg tccggtagct
     2641 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     2701 tgcaactcgt aggacaggtg ccggcagcgc ccaacagtcc cccggccacg gggcctgcca
     2761 ccatacccac gccgaaacaa gcgccctgca ccattatgtt ccggatctgc atcgcaggat
     2821 gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctaa ccgtttttat
     2881 caggctctgg gaggcagaat aaatgatcat atcgtcaatt attacctcca cggggagagc
     2941 ctgagcaaac tggcctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa
     3001 taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac
     3061 gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag
     3121 gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaa
//
