LOCUS       62056_20230        6535 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(741..1329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1503..2360)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2361..2465)
                     /label=AmpR promoter
     CDS             complement(2632..4005)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     CDS             complement(4208..5209)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
ORIGIN
        1 aattccgtta agatagtcga ctcctatatc aataccaatc gagtagcctg cattcttatt
       61 aaaaacaagc attacaggtc ttctgccgcc tctagattgc cctgccccaa tttcaaaaat
      121 aaaatctttt tcaagcagtg tatttacttg agaggagaca gtagacttgt ttaatcctgt
      181 aatctcagag agagttgccc tggagacagg ggagttcttc aaaatttcat ctaatattaa
      241 tttttgattc atttttttta ctaaagcttg atctgcaatt tgaataataa ccactccttt
      301 gtttatccac cgaactaagt tggtgttttt tgaagcttga attagatatt taaaagtatc
      361 atatctaata ttataactaa attttctaaa aaaaacattg aaataaacat ttattttgta
      421 tatgatgaga taaagttagt ttattggata aacaaactaa ctcaattaag atagttgatg
      481 gataaacttg ttcacttaaa tcaaaggggg aaatgtacaa tggtccaaac tagttcgaag
      541 atctccggag ctcccgggat ccggtaccgg ccgcatgccg gctaaacctc gcgaacggat
      601 tcaccggtcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
      661 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct
      721 cgggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     1201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1381 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1441 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1501 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1561 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1621 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1681 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1741 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1801 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1861 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1921 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1981 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2041 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2101 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2161 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2221 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2281 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2341 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2401 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2461 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     2521 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cctgttataa
     2581 aaaaaggatc aattttgaac tctctcccaa agttgatccc ttaacgattt agaaatccct
     2641 ttgagaatgt ttatatacat tcaaggtaac cagccaacta atgacaatga ttcctgaaaa
     2701 aagtaataac aaattactat acagataagt tgactgatca acttccatag gtaacaacct
     2761 ttgatcaagt aagggtatgg ataataaacc acctacaatt gcaatacctg ttccctctga
     2821 taaaaagctg gtaaagttaa gcaaactcat tccagcacca gcttcctgct gtttcaagct
     2881 acttgaaaca attgttgata taactgtttt ggtgaacgaa agcccaccta aaacaaatac
     2941 gattataatt gtcatgaacc atgatgttgt ttctaaaaga aaggaagcag ttaaaaagct
     3001 aacagaaaga aatgtaactc cgatgtttaa cacgtataaa ggacctcttc tatcaacaag
     3061 tatcccacca atgtagccga aaataatgac actcattgtt ccagggaaaa taattacact
     3121 tccgatttcg gcagtactta gctggtgaac atctttcatc atataaggaa ccatagagac
     3181 aaaccctgct actgttccaa atataattcc cccacaaaga actccaatca taaaaggtat
     3241 atttttccct aatccgggat caacaaaagg atctgttact ttcctgatat gttttacaaa
     3301 tatcaggaat gacagcacgc taacgataag aaaagaaatg ctatatgatg ttgtaaacaa
     3361 cataaaaaat acaatgccta cagacattag tataattcct ttgatatcaa aatgaccttt
     3421 tatccttact tctttcttta ataatttcat aagaaacgga acagtgataa ttgttatcat
     3481 aggaatgagt agaagatagg accaatgaat ataatgggct atcattccac caatcgctgg
     3541 accgactcct tctcccatgg ctactatcga tccaataaga ccaaatgctt tacccctatt
     3601 ttcctttgga atatagcgcg caactacaac cattacgagt gctggaaatg cagctgcacc
     3661 agccccttga ataaaacgag ccataataag taaggaaaag aaagaatggc caacaaaccc
     3721 aattaccgac ccgaaacaat ttattataat tccaaatagg agtaaccttt tgatgcctaa
     3781 ttgatcagat agctttccat atacagctgt tccaatggaa aaggttaaca taaaggctgt
     3841 gttcacccag tttgtactcg caggtggttt attaaaatca tttgcaatat caggtaatga
     3901 gacgttcaaa accatttcat ttaatacgct aaaaaaagat aaaatgcaaa gccaaattaa
     3961 aatttggttg tgtcgtaaat tcgattgtga ataggatgta ttcacatttc accctccaat
     4021 aatgagggca gacgtagttt atagggttaa tgatacgctt ccctctttta attgaaccct
     4081 gttacattca ttacacttca taattaattc ctcctaaact tgattaaaac attttaccac
     4141 atataaacta agttttaaat tcagtatttc atcacttata caacaatatg gcccgtttgt
     4201 tgaactactc tttaataaaa taatttttcc gttcccaatt ccacattgca ataatagaaa
     4261 atccatcttc atcggctttt tcgtcatcat ctgtatgaat caaatcgcct tcttctgtgt
     4321 catcaaggtt taatttttta tgtatttctt ttaacaaacc accataggag attaaccttt
     4381 tacggtgtaa accttcctcc aaatcagaca aacgtttcaa attcttttct tcatcatcgg
     4441 tcataaaatc cgtatccttt acaggatatt ttgcagtttc gtcaattgcc gattgtatat
     4501 ccgatttata tttatttttc ggtcgaatca tttgaacttt tacatttgga tcatagtcta
     4561 atttcattgc ctttttccaa aattgaatcc attgtttttg attcacgtag ttttctgtat
     4621 tcttaaaata agttggttcc acacatacca atacatgcat gtgctgatta taagaattat
     4681 ctttattatt tattgtcact tccgttgcac gcataaaacc aacaagattt ttattaattt
     4741 ttttatattg catcattcgg cgaaatcctt gagccatatc tgacaaactc ttatttaatt
     4801 cttcgccatc ataaacattt ttaactgtta atgtgagaaa caaccaacga actgttggct
     4861 tttgtttaat aacttcagca acaacctttt gtgactgaat gccatgtttc attgctctcc
     4921 tccagttgca cattggacaa agcctggatt tacaaaacca cactcgatac aactttcttt
     4981 cgcctgtttc acgattttgt ttatactcta atatttcagc acaatctttt actctttcag
     5041 cctttttaaa ttcaagaata tgcagaagtt caaagtaatc aacattagcg attttctttt
     5101 ctctccatgg tctcactttt ccactttttg tcttgtccac taaaaccctt gatttttcat
     5161 ctgaataaat gctactatta ggacacataa tattaaaaga aacccccatc tatttagtta
     5221 tttgtttggt cacttataac tttaacagat ggggtttttc tgtgcaacca attttaaggg
     5281 ttttcaatac tttaaaacac atacatacca acacttcaac gcacctttca gcaactaaaa
     5341 taaaaatgac gttatttcta tatgtatcaa gataagaaag aacaagttca aaaccatcaa
     5401 aaaaagacac cttttcaggt gcttttttta ttttataaac tcattccctg atctcgactt
     5461 cgttcttttt ttacctctcg gttatgagtt agttcaaatt cgttcttttt aggttctaaa
     5521 tcgtgttttt cttggaattg tgctgtttta tcctttacct tgtctacaaa ccccttaaaa
     5581 acgtttttaa aggcttttaa gccgtctgta cgttccttaa gatcaacgtg atataggttt
     5641 gctaaccttt gcgttcactt aactaactta taggggtaac acttaaaaaa gaatcaataa
     5701 cgatagaaac cgctcctaaa gcaggtgcat tttttcctaa cgaagaaggc aatagttcac
     5761 atttattgtc taaatgagaa tggactctag aagaaacttc gtttttaatc gtatttaaaa
     5821 caatgggatg agattcaatt atatgatttc tcaagataac agcttctata tcaaatgtat
     5881 taaggatatt ggttaatcca attccgatat aaaagccaaa gttttgaagt gcatttaaca
     5941 tttctacatc atttttattt gcgcgttcca caatctcttt tcgagaaata ttcttttctt
     6001 ctttagagag cgaagccagt aacgcttttt cagaagcata taattcccaa cagcctcgat
     6061 ttccacagct gcatttgggt ccattaaaat ctatcgtcat atgacccatt tccccagaaa
     6121 aaccctgaac acctttatac aattcgttgt taataacaag tccagttcca attccgatat
     6181 taatactgat gtaaacgatg ttttcatagt tttttgtcat accaaatact ttttcaccgt
     6241 atgctcctgc attagcttca ttttcaacaa aaaccggaac attaaactca ctctcaatta
     6301 aaaactgcaa atctttgata ttccaattta agttaggcat gaaaataatt tgctgatgac
     6361 gatctacaag gcctggaaca caaattccta ttccgactag accataaggg gactcaggca
     6421 tatgggttac aaaaccatga ataagtgcaa ataaaatctc ttttacttca ctagcggaag
     6481 aactagacaa gtcagaagtc ttctcgagaa taatatttcc ttctaagtcg gttag
//
