LOCUS       V003025                10870 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003025
VERSION     V003025
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Chromosomal complementation using Tn7 transposon vectors in
            Enterobacteriaceae
  JOURNAL   Appl. Environ. Microbiol. 78 (17), 6001-6008 (2012)
   PUBMED   22706059
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2012) Microbiology and Biothechnology,
            INRS-Institut Armand-Frappier, 531, boul. des Prairies, Laval,
            Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 10870)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10870)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "17";
            pages: "6001-6008"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-JAN-2012) Microbiology and Biothechnology, INRS-Institut
            Armand-Frappier, 531, boul. des Prairies, Laval, Quebec H7V 1B7,
            Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(281..1807)
                     /codon_start=1
                     /gene="tnsD"
                     /product="Tn7 transposase subunit"
                     /label="tnsD"
                     /protein_id="AFM78104.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVAT
                     LGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAV
                     HLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDR
                     AVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFY
                     QRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKA
                     FSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQ
                     LVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIA
                     VRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDY
                     QIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            complement(281..1807)
                     /gene="tnsD"
                     /label="tnsD"
     CDS             complement(1813..3477)
                     /gene="tnsC"
                     /label="Transposon Tn7 transposition protein TnsC"
                     /note="Transposon Tn7 transposition protein TnsC from
                     Escherichia coli. Accession#: P05846"
     CDS             complement(3477..5582)
                     /gene="tnsB"
                     /label="Transposon Tn7 transposition protein TnsB"
                     /note="Transposon Tn7 transposition protein TnsB from
                     Escherichia coli. Accession#: P13989"
     mobile_element  6300..6524
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     protein_bind    6735..6782
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(6847..7080)
                     /label="TpR"
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(7407..7435)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     misc_recomb     7487..7534
                     /note="FRT; Flp recombinase target site"
     protein_bind    7487..7534
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(7596..7614)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7650..7683)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             7851..8642
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      9511..9733
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9781..10728
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
ORIGIN
        1 cctgcaggtc gactctagag gatcccccga ttattttggc tcgttgaaga tccgatgctt
       61 caaactcaat cacacgatcc gccttattta tttgctttgt tgtactgtta accaccttag
      121 ctcgacttaa taaaggcaaa tgagtaaatc gagtccattt ccgttcttgc attggattaa
      181 accaaacaaa aatacgccac tccttatgac ccgagttacg gaataaatga ccaatatgta
      241 caacctgcac attgtcatta aatgtagcta gcctaaccac tcactcttcc ccataaacca
      301 tttccaagaa tctttgtgct tcctcagtta tccgctcttt agataacgtt gcacttctta
      361 ataatcgcca gcgcctaagc tcaacatctt cctgtttaag cttaataaaa gcttggctaa
      421 tccgtctaat ttgataatct tccacactct ctgagtaacg ctttaagcaa agcgctacca
      481 aaggcagttt ctgtagattt tttgcaagag aggttccgtt aggagtttgc tttaacagcc
      541 agctcgatgt cgctcttggg tgatcaaggc tactatctag acgctttatg attcttaata
      601 gttgtcgtac agcaattcga tctctttggt tccaatcaac tctaggggcg ggtgccagac
      661 gctcttgttg atgctgttga ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc
      721 aagcgtatag caccccaccc tctaaagact gtcttgccgc cttaattcct tggtatttat
      781 gcactagttg ctgccagtct ttatgcttaa cagataaatc ttcagagtta ggttgcacag
      841 actggctaac aggtcttgtc gttatagagt gctcagtaag agcacttgcc tgctgtagcg
      901 cttctataac cgttagtttt ggcaataagg cttgccacac aatactatgc tgtaaataac
      961 taaaggcttt tctatgctta cggaatatac ttttcagcca acacgtgtcc ttgttctctg
     1021 ccaactttaa atccagtttc tctagtgcct catcactaaa agtctgcctc actctctccg
     1081 ccaccaagtc atgacgaatg tgcttgcttt tggttagccc tagatcctgc gctaagcgct
     1141 gataaaacag cgtccactgc tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta
     1201 acagaggggc tatataagca gctagtgctg ttaattgaga tagggagtct ttggggtagt
     1261 ctgaaagcag ctcagtatga cccaaagccc aaaattgatg tcggtgatca tctacagctc
     1321 tatcaaagaa gactaaagca ccgtgttttg gacaatatgg caaagcgggc aaataccaat
     1381 ctcgttgcca aaaggcttcc ccatacctat ttagctgaag agcaacgcaa tcagggcagt
     1441 atctaaagcg gttatcgctc ttaactctag aagcagcgac tcctagcatt aaatgcaccg
     1501 caccttgcgc ttggtactcc attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa
     1561 acggagcata taaagggaat aaggtatgct cataaataag ctgctgaaca gcgtaacgtc
     1621 ctgtttgatg tagatgtctt gctatcacac ctaaatgcga gggcagacct aaggtagcga
     1681 ccaccttgcg gttgccatac acctcatcca acagctgctt aggactaaca atcccttgat
     1741 aaacgcctgc ccgtgcaata gtgctataaa tcagctcatt cgagtacgga acaggaaagt
     1801 ttctcatggc taacctgctt gtttaaataa gctcgccata tccactatta cccctttccc
     1861 ttttaaccgt tcatgcatgg ttttttcagg ttggcgttgt gaatagatat aacgtaaatc
     1921 cgtatcaggc aagctatccc agtcgctggg cttgactacc tttatagccg aaaccttttt
     1981 actcttggag ggcttttcta gttctgacac taccgtttcg ccttccatca accactgcaa
     2041 aacaagaggc agtaactttt gtcttgtcat cgttggattc tggctaaacg cttttttaat
     2101 agtgggaatt aacaggcttg aatcgtaatc ctctttcagc atcagatata aatgacgctg
     2161 atcttcggta tctaactctt gaagggcttt ttcttctggt gtttgttctt gtatcgctgc
     2221 gatatctagc tgaagttgga ttaaccgttt atcaatctcg ggaacgacta gatcagaata
     2281 acgagcaatg cgttctggga tacccgagcg taatgcctct agcatggggt gcacaggctt
     2341 taactcatct tgatacactt gccgcaataa accagcggta atacgctcat tgcctaaagc
     2401 tagcgcacgg agctgagcga gtacaaaaag ttttactaca atgtccatca ctccttggct
     2461 tagctcatac cacacatcac ggacctcatc cgataacagc gcatctttgc gttgtaaaag
     2521 ctgtaattgc caaagattat ccgtaaaagc gatccactct tgattgggct ttccacgttg
     2581 cgtttgttgt ataggatccc agaatatagc tccaaaccct gcccctctac gtgcagaccg
     2641 caaatcagcc tcaaaaatct ctcgtgcttt aggggtacca atcaacatca ctggtacgcc
     2701 aataatattc accatcgtca caaaaaagtt cagcatctct tgagatccac ccgaacgaga
     2761 gcggcttaaa tgctgaattt catcaataac caacaaccct aaagcatgtg cattggctat
     2821 ttgcgacatc aaagccaaca tggtttctat accatgacgt tttaagccat aacgacgctc
     2881 atagttcgag cccaaggctc gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag
     2941 cgaaccatta tgcgagcagt ctattttcaa atacaccacc tgctctacat tgagttcacg
     3001 atggtaaatc acctgaggat acgtggctag aatacgatga agagaggtcg tcttcccact
     3061 accagaacaa ccaattaaca ataagctttg tgccgtagat cgtgcctcct caaagcgaaa
     3121 tgtctccaac tctcccgttt gaacacgctc ataaccattt tgtaaatgct tttgtaaatc
     3181 tcctgtttta ggatttctgc ctacgtagcc acctcgaatc atgaccgaaa tacgctcact
     3241 tagtagcaaa tgcgtaccta atggctgaaa atagtcatct ggaatacgac aaatggtatg
     3301 agctctgata acacgggact tttgcaagtc agaggaagta agctgtaaag aggatttcag
     3361 tgatgcagca ctattcactg actcttgtaa tggtggtaag gcctcgataa aaggattatc
     3421 acgataagcc tctacccccg tatcacgata aactgcttga atccgggtag cactcatgac
     3481 tcatcctttt ctggtggatc ctgaaataat tcaggcacgt atgtgggtag gctgtaatct
     3541 tcttgatcat ccgcttccac tgcgttgaaa ggaataactt tagcctcatc acctgaagag
     3601 cttggcttca aatgctccgc acgtttttta cgctccgagg tcacggcttc ttttttatta
     3661 gttttaatct gcttaatgcg tgttgatttg ggctcagtag tactgggcgt taacttattc
     3721 gctttctgaa tggtttgctg aataaacgcc tcaagctccc tgcgtttagt taactcatcc
     3781 tgcttcgcat tggctttatt gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat
     3841 gagagacctt taaactgccg actacgttcc gtcagattac agcgccaaaa tacacggctg
     3901 ccaacttgcg gaaacaaata aatcgtatca accagcactg ggtcataagc cgcttctaaa
     3961 tgttgaggtc tagctatatc agtgctccgc tgcaaccaac cctcacgcag aatctctgac
     4021 cccgagtaat acaaacccca caaattaacg ccaaatgaag aaatagagac ctttcggcga
     4081 ggcagtaacg ctactcgcaa ctgctcttgc tccacagccc ttaaactacc tgtacgatgc
     4141 tgcatacccc attgccatag ctggacagga atagacggta aatctgtagg aaaatcagca
     4201 tctcgatcgt atttatccat caccagatgg ttatttctga ataagatcgt acgcaaaata
     4261 atttgtgtga actcaaatac cgacagagat gcatctaacc tatagtctgt ttcaccatgg
     4321 cttttgatcc gactgccctc tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt
     4381 gttctaaaag tgctttccac tatgccttta gcatcgccac gtctaggtgg agcactttcc
     4441 actcgcacat taaaactaga aactaaggct tcgacctgat gactcattaa ttcgccacgg
     4501 tccgctagca acacatctgg caaacctaca cacggccagt ctgagctact aatctcaata
     4561 tcatgctggg cacaaatggc cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc
     4621 accacataag acggattttc aaagccgata taaaagcccg tgatcatccg actaaacaca
     4681 tcaatcacaa tgtaaagcgt tggtcttcct atgatttttt ggcgatcatg atgatccact
     4741 aaataaatat cagcaatcgt ggcatcaatc tcataacgac tcccagggcc taacgcctga
     4801 gaagtggctg tactacttaa gggtcgtacg tcttttttat atacccctgc tttaactcta
     4861 gactttaagc gctgagcttt agggtattct cgatcataaa aataacgaaa ctgacgtagt
     4921 gttgggtaat cctcttgggg aatgcgagga aaatactgag caaacaagtc cacaaatcgt
     4981 ctataggcaa cggtggtctt tgtacctttt tgatttaaca ggtgcttttc tatggtcaac
     5041 ctaaaaaggc gttcaatctc gggcgttacc ttggttcctt cacccttacc atattctctg
     5101 gctcggccaa tctttgctgt tcctgtcgct gaacgtcttt cccctggtgc accgctgttt
     5161 ttgtagtcag gaattaatgc attaggcgtt tgaccacgct gccagtaacg gcgtaacaac
     5221 ttataaactg tagccttagt aaccttatgt tcttggacca catgctcaac gagttcgctt
     5281 ctgactttag ggtcgaaacg atccttacta ttaataatag gaagaatttt tcgataatcc
     5341 tcctcgcgct tctggaagct gacagaatct acagacggct cttctaaatc aagatgtaca
     5401 taagggtcat cagttctaac taagcggcct tcatctaaat actgcattag caacaactca
     5461 gccctagctt gtggaactcc tttatcagcg cttatttgca tccagacaac ttggccatcc
     5521 tctatagcca aaatgcgata cggatcatta tcaaatagca caacctcatt aatttgccac
     5581 atagcgcaac tcctccatat tcactacttg gctaatacag agatctgcac acttatttgc
     5641 cctgaaagac ttataaatat tgaacttaat aaaaccattt gcggttaagg ctcgtatctc
     5701 actcaatgtt ttgcctaact ccaaatcata agcaatatca acctgcttac agacattgat
     5761 aatgttttca tctccttttt cttgcaggat atgggccaat ggggatagtt gtgctaaaag
     5821 ctccgcagaa acttcttctg ttttcactga ataaagccat tcaatatttt cttttactac
     5881 gggatttatt tctttatcag taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg
     5941 acgctctagt tctagttttt ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac
     6001 ttgaatagca aactgctcaa aaggaccatc tttgcagtcc actaaaaaat cagtagacat
     6061 aacctgatct acaccacgaa taacaggatg cttaatacca ctatctattg caatctgcct
     6121 ggtatcacta ggtaataagg ggaactgctc gcgtatatct agcacgctgc tctcccactc
     6181 aagactgaga aaaacagcaa gctctaagtc agatagcaaa tgatggactc gtcccgtctt
     6241 atgagaataa atacggtggg aacgacctga agaaggaact tcttgtactg ttagccatgg
     6301 aatatagtct ttaccatgcc cttggccacg cccctcttta atacgacggg caatttgcac
     6361 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
     6421 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
     6481 ttagatctat tttgttccct ttaagacttt attgtccgcc cacacccgct tacgcagggc
     6541 atccatttat tactcaaccg taaccgattt tgccaggtta cgcggctggt caacgtcggt
     6601 gcctttgatc agcgcgacat ggtaagccag cagctgcagc ggaacggtgt agaagatcgg
     6661 tgcaatcacc tcttccacat gcggcatctc gactctagag gatccccggg taccgagctc
     6721 gaattgggga tcttgaagta cctattccga agttcctatt ctctagaaag tataggaact
     6781 tcagagcgct tttgaagctg atgtgcttaa aaacttactc aatggaataa ttctagataa
     6841 ttcttaggcc acacgttcaa gtgcagccac aggataaatt tgcactgagc ctgggtggga
     6901 ttcggactcg accgcatagc cttcaggagt gagttttgtg caataccaac cgacgacttg
     6961 accctgccaa gcggcaccag atttcttgcg tacgcgatcc cctaagccaa aggtggcact
     7021 caggggaagc gcaaactgcc ctgcaacggg agcgttggct tcatcgctac tttgacccat
     7081 gtcgaatcct tcttgtgaat ctattatggc gacaagcaaa tcgagctctg actgcctacc
     7141 ccacaacaac tactagaaag caccagcaca acggctgcct aactttgttt tagggcgact
     7201 accctgctgc gtaacatcgt tgctgttcca taacatcaaa catcgaccca cggcgtaacg
     7261 cgcttagcta gcttggatgc ccgaggctag actgtacaaa aaaacagtca taacaagcca
     7321 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     7381 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     7441 ttcgtgaatt atccattgct gttgacaaag ggaatcaggg gatcttgaag ttcctattcc
     7501 gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaagc taattcgagc
     7561 tcggtacccg ggtaccgagc tcgaattccg gtctccctat agtgagtcgt attaatttcg
     7621 ataagccaga tcaagcttgt cgacaattcg aagttcctat actttctaga gaataggaac
     7681 ttcggatcaa ttcatcgatg caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7741 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7801 aatgcttcaa taatctagtg gatcaagaga caggatgagg atcgtttcgc atgattgaac
     7861 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7921 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7981 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactc caagacgagg
     8041 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     8101 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     8161 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     8221 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     8281 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     8341 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg gatgcccgac ggcgaggatc
     8401 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     8461 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     8521 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     8581 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     8641 tctgagcggg actctggggt tcgataccgt caaaacttca tttttaattt ttgcggcccg
     8701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9001 taacgtgagt tttcgttcca ctgagcgtca gaccccgttg atgataccgc tgccttactg
     9061 ggtgcattag ccagtctgaa tgacctgtca cgggataatc cgaagtggtc agactggaaa
     9121 atcagagggc aggaactgct gaacagcaaa aagtcagata gcaccacata gcagacccgc
     9181 cataaaacgc cctgagaagc ccgtgacggg cttttcttgt attatgggta gtttccttgc
     9241 atgaatccat aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag
     9301 cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa
     9361 aaggaatatt cagcgatttg cccgattgcg gccgcaaccg agcttgcgag ggtgctactt
     9421 aagcctttag ggttttaagg tctgttttgt agaggagcaa acagcgtttg cgacatcctt
     9481 ttgtaatact gcggaactga ctaaagtagt gagttataca cagggctggg atctattctt
     9541 tttatctttt tttattcttt ctttattcta taaattataa ccacttgaat ataaacaaaa
     9601 aaaacacaca aaggtctagc ggaatttaca gagggtctag cagaatttac aagttttcca
     9661 gcaaaggtct agcagaattt acagataccc acaactcaaa ggaaaaggac tagtaattat
     9721 cattgactag cccatctcaa ttggtatagt gattaaaatc acctagacca attgagatgt
     9781 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
     9841 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
     9901 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgttca gatgatgaac
     9961 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
    10021 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
    10081 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
    10141 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
    10201 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
    10261 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
    10321 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
    10381 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
    10441 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
    10501 atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctacg taacggacta
    10561 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
    10621 tttttgagtg acatgcaaag taagcatgat ctcaatggtt cgttctcatg gctcacgcaa
    10681 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
    10741 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca
    10801 ccgttacata tcaaagggaa aactgtccat acccatgggc tagctgatca gccagtgcca
    10861 agcttgcatg
//
