LOCUS       V003029                 6903 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003029
VERSION     V003029
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
   PUBMED   21249169
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 6903)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio";
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av.,
            Montreal, QC H4P2R2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            415..2889
                     /gene="STE11"
                     /label="STE11"
     CDS             415..2886
                     /codon_start=1
                     /gene="STE11"
                     /product="STE11"
                     /label="STE11"
                     /note="ORF19.844; Candida albicans MAPKKK"
                     /protein_id="ADW40670.1"
                     /translation="MTEINDSTENITRIDRNDAKELKHWLAKANCQHHYSKFIENGITM
                     DLLPELDTGSLKELGISKLGDRLRLEIAISNLKAENLKELISVDEIYQLLNLNPQPSGS
                     LTNLNNNNNSLLDVSNSNEGTLTQLSQNNSSNNLPISSMNSSTNNINGNTINSSSGNVV
                     GNSASSKDSSKTITFILQDGSTKRVNATGCFNAQAIKRRVLKKLGFKPDEIQFDTYVHS
                     VPHGVENVLYGKTEPTITLLYDVELVTICYSPERLEKYRIMLVPKNEPPTPAAIETSKK
                     IMSKYKKQVSGSKAELQEEVNQLHTKDVQAHRSTLRNFFGQRPPSELISSNLAEYFPDT
                     RQKDLEQTVRNSVRHSVRLSRRFNLPAGAFSSSVYSLNKRQSILSNSTGFPSNRTMSIS
                     SGEQQSGVFNTSGGGSGGLRRAQRTIGDVMVNSITAIDEVVNSNDTFSVFNKPSTSFSN
                     QPVQPLNNHDSRSIISSKSLSGGTALHRISISPNYNDNPAHDRHSTIELLNPSDSEGED
                     GEEDGEFGISDYAGLSSSSGENGPPENWLKGARIGSGSFGTVYLGMNPFTGELMAVKQI
                     PLVNENGNNSDTENIQNSMQEQQREMMLLKELNHENIVRYFGSTTDENFLNIFLEYVPG
                     GSVQSMLNSYGPFEEPLIRNFVRQVLIGLSYLHGEDIIHRDIKGANILIDIKGTVKIGD
                     FGISKKVSAIDEEDEDFKKTGKRASLQGSVFWMAPEVVKQTTYTKKADIWSVGCLIVEM
                     FTGRHPFPELSQMQALFKIGNHITPQIPEWCTNEAKEFLKKTFEINFEMRPDAIELLAE
                     QFLNPLIMSKQ"
     CDS             3527..4336
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(4541..4559)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4817..5405)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5579..6436)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6437..6541)
                     /label="AmpR promoter"
     promoter        6887..6903
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gctttttttg attgttactt attattaagc tcggttaaag
       61 tatctttagt ttaagaaatg atagactgtc attcaacatt caattccgaa acttatctga
      121 ataatacacc accacaacaa gttgtacaaa aaaaaagaag gacaatagat atttgaggaa
      181 aagaaaaaaa aaacagtgag aaagtgtgag ggaaaaaaaa ttattgtgac atgggctctt
      241 tcttcaaatc ccgtctattc atattcatgc aaacaacaac aaaagtcgtc caggtttctg
      301 ttctttcatg gtattctttt ttttaagatt tgattcaaat taagtttaaa gatcatatag
      361 aaaccctaat acattagttc gtgtgtatat ggtactcaaa tctaacaaac agtcatgaca
      421 gagattaatg attctacaga aaacataacc cgaatagatc gaaatgatgc aaaggaatta
      481 aaacattggt tagccaaggc aaattgccaa catcactatt ccaagtttat tgaaaatgga
      541 atcactatgg atttactacc ggaattggat acaggttcat taaaggaatt gggtatttca
      601 aaattaggag accggttaag attagaaatt gctatatcca acttgaaggc cgaaaatttg
      661 aaggaactaa taagtgttga cgaaatatat caattgttaa atttaaatcc tcaaccaagt
      721 ggatctttga caaacttgaa taacaataat aatagtttgt tagatgtatc caacagtaac
      781 gaaggaacat taacccaact aagtcaaaat aactccagta ataatttgcc tattagtagc
      841 atgaattcca gcactaacaa tataaatggt aataccatca atagtagctc tggaaacgtt
      901 gtcggtaata gtgcttccag taaagattcc agtaagacca ttacgttcat tttacaagat
      961 ggatcaacta aacgtgtaaa tgccactggt tgttttaatg cccaagcaat taaaaggaga
     1021 gttttgaaga aattagggtt caagcctgat gaaattcaat ttgatactta tgtccattca
     1081 gttccacacg gtgttgaaaa tgtattatat gggaaaaccg aacctactat tactttatta
     1141 tacgatgttg aattagttac catttgttat tcaccagaac ggttggagaa atatcgtata
     1201 atgttggtac ctaaaaatga acctcctacg ccagcagcca ttgaaacatc gaaaaaaatt
     1261 atgtcaaaat ataaaaagca agtttctgga tctaaagctg aattgcaaga agaagttaat
     1321 caacttcata ccaaagatgt tcaagcacat agatcgacct tgagaaattt ctttggacaa
     1381 agacctccta gtgaattgat ttcatcgaat ttagcagagt atttccctga cacaagacaa
     1441 aaagatttgg aacaaaccgt tagaaactct gttagacata gtgttagatt atctcgaaga
     1501 tttaatttac cagcgggggc attttcttca agcgtatatt ctctcaacaa gagacaatct
     1561 attttgagta attcaaccgg ttttccttca aatcgtacaa tgtccatttc ttctggtgaa
     1621 caacaaagtg gggtatttaa tactagcgga ggtggtagtg gtgggttaag aagagcacaa
     1681 cgaaccattg gggatgtgat ggtgaatagt attactgcta ttgatgaagt agtgaattca
     1741 aacgatacat tttctgtatt taataaacct tcaacgtcat tttcaaatca gccagtacaa
     1801 ccattaaata atcatgattc gagatccata atatcgtcaa aatcattaag tgggggaact
     1861 gcattgcata ggatttctat atctccaaat tataacgaca accctgctca tgatcgccat
     1921 tctacaattg aattattgaa tccatctgac tcagagggtg aagatggtga ggaagatgga
     1981 gaatttggta tttctgacta tgccggattg agttctagtt ctggggagaa tggtccgcct
     2041 gaaaattggt taaagggggc tagaattggt tctggtagtt ttggtacagt gtatttgggg
     2101 atgaatccat ttacagggga gttgatggca gtaaaacaaa tcccgttagt caatgagaat
     2161 gggaataata gtgatactga aaacatccaa aactccatgc aagaacaaca acgagaaatg
     2221 atgttattaa aggaattgaa tcatgaaaat atagttcgct attttggatc tacaaccgat
     2281 gagaattttt taaatatatt tttagaatat gtgcccggag gttcagttca atcgatgttg
     2341 aattcatatg ggccatttga agagccattg ataagaaatt ttgttcgaca agtattgatt
     2401 gggttaagtt atttgcatgg tgaagatatt attcatcgag atattaaagg tgccaacata
     2461 ttgattgata tcaaaggtac tgtcaaaatt ggtgattttg gtattagtaa aaaagtcagt
     2521 gccattgatg aagaagatga agatttcaaa aaaacaggta aaagagcatc tttacaagga
     2581 tcagtattct ggatggcacc tgaagtggtt aagcaaacta catatactaa aaaagcggat
     2641 atttggtcag ttggatgttt gattgtggaa atgtttactg gtagacatcc attcccagag
     2701 ttgagtcaaa tgcaagcatt atttaagatt ggtaatcata ttaccccgca aattcctgaa
     2761 tggtgtacta atgaagccaa agaatttcta aagaaaacat ttgagattaa ttttgaaatg
     2821 agaccagatg caattgaatt attagcagaa caatttttaa atccattaat tatgtcgaaa
     2881 caatgataaa tatataaagt tatactgtac cacgtgattg ttgtatgacg tagtaggaac
     2941 aatctagaga gatgttttta attattgttg ttgtaattta cttgtgtgtg tgtgtctttt
     3001 tgttcatttt attctagtca gccttttttt atttggtgtg tgtgtgagtg gggtgttgca
     3061 tatatttggt ggggaagcag tgggagggag ggctaagctt cgtacgctgc aggtcgacgg
     3121 atccggatgg tataaacgga aacaaaaaaa agagctggta ctactttctt taaaattatt
     3181 ttattatttg attttattta atagtatata ttatattttg aacgtagatt attttgttga
     3241 aagttgctgt agtgccattg attcgtaaca ctaattctgt attagtcatt cctcttgttt
     3301 gatagtatcc aaaaaaacgg ctattttttt gcaatcttat ttcctgcata ttatacagat
     3361 aacataatga aagaaaaaat cttttttttt gttcttcaat gatgatttca accattcttt
     3421 taaacattga tcaattcctg agcaacaacc ccatacacac tggtttatat accgcccctt
     3481 ttacagttga agaaagaaat agaaatagaa atagcaaaca aaagatatga cagtcaacac
     3541 taagacctat agtgagagag cagaaactca tgcctcacca gtagcacagc gattatttcg
     3601 attaatggaa ctgaagaaaa ccaatttatg tgcatcaatt gacgttgata ccactaagga
     3661 attccttgaa ttaattgata aattaggtcc ttatgtatgc ttaatcaaga ctcatattga
     3721 tataatcaat gatttttcct atgaatccac tattgaacca ttattagaac tttcacgtaa
     3781 acatcaattt atgatttttg aagatagaaa atttgctgat attggtaata ccgtaaagaa
     3841 acaatatatt ggtggagttt ataaaattag tagttgggca gatattacca atgctcatgg
     3901 tgtcactggg aatggagtgg ttgaaggatt aaaacaggga gctaaagaaa ccaccaccaa
     3961 ccaagagcca agagggttat tgatgttagc tgaattatca tcagtgggat cattagcata
     4021 tggagaatat tctcaaaaaa ctgttgaaat tgctaaatcc gataaggaat ttgttattgg
     4081 atttattgcc caacgtgata tgggtggcca agaagaagga tttgattggc ttattatgac
     4141 acctggagtt ggattagatg ataaaggtga tggattagga caacaatata gaactgttga
     4201 tgaagttgtt agcactggaa ctgatattat cattgttggt agaggattgt ttggtaaagg
     4261 aagagatcca gatattgaag gtaaaaggta tagaaatgct ggttggaatg cttatttgaa
     4321 aaagactggc caattataaa tgtgaagggg gagattttca ctttattaga tttgtatata
     4381 tgtagaataa ataaataaat aagttaaata aataattaaa taagggtggt aattattact
     4441 atttacaatc aaaggtggtc cttctagttt aaacgagctc gaattcatcg atgatatcag
     4501 atccactagt ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa
     4561 tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4981 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     5041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5221 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6541 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     6601 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     6661 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     6721 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     6781 actatgcggc atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag
     6841 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact
     6901 ata
//
