LOCUS       pSTBlue-1.        3851 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Multi-purpose bacterial cloning and expression vector with a 
            versatile MCS, T7 and SP6 promoters, and ampicillin and kanamycin 
            resistance genes.
ACCESSION   .
VERSION     .
KEYWORDS    pSTBlue-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    51..178
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(184..202)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(216..232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(373..828)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        855..959
                     /label=AmpR promoter
     CDS             960..1817
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             1966..2778
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2866..3454
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3742..3763
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3778..3808
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3816..3832
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaccatga ttacgccaag ctctaatacg actcactata gggaaagctc ggtaccacgc
       61 atgctgcaga cgcgttacgt atcggatcca gaattcgtga tatctgaatt cgtcgacaag
      121 cttctcgagc ctaggctagc tctagaccac acgtgtgggg gcccgagctc gcggccgctg
      181 tattctatag tgtcacctaa atggccgcac aattcactgg ccgtcgtttt acaacgtcgt
      241 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      301 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      361 aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
      421 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
      481 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
      541 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
      601 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
      661 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
      721 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
      781 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
      841 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      901 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      961 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1021 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1081 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1141 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1201 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1261 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1321 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1381 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1441 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1501 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1561 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1621 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1681 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1741 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1801 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1861 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1921 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1981 cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg
     2041 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
     2101 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2161 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2221 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca
     2281 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2341 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2401 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2461 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2521 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2581 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2641 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2701 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2761 atgctcgatg agtttttcta agaattaatt catgaccaaa atcccttaac gtgagttttc
     2821 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2881 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2941 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3001 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3061 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3121 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3181 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3241 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3301 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3361 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3421 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3481 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3541 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3601 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3661 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     3721 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     3781 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     3841 aggaaacagc t
//
