LOCUS       62056_20215        4739 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        109..140
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             173..889
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    complement(936..1057)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1251..1608
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1639..2430
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2458..2506
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2833..3421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3624..4481)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    4648..4739
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg ctagcctcga gtgcttcccg aacgttgctt cccgaacgtt
       61 gcttcccgaa cgttgcttcc gaacgtagat ctgcagaagc ttagacacta gagggtatat
      121 aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggtgag
      181 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      241 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      301 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      361 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      421 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      481 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      541 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      601 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      661 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      721 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      781 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      841 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aataattcta
      901 gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
      961 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1021 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     1081 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     1141 atgtggtaaa atcgataagg atccgtttgc gtattgggcg ctcttccgct gatctgcgca
     1201 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt tagctacctt ctgaggcgga
     1261 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     1321 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     1381 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     1441 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     1501 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     1561 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgattct
     1621 tctgacacta gcgccaccat gatcgaacaa gacggcctcc atgctggcag tcccgcagct
     1681 tgggtcgaac gcttgttcgg gtacgactgg gcccagcaga ccatcggatg tagcgatgcg
     1741 gccgtgttcc gtctaagcgc tcaaggccgg cccgtgctgt tcgtgaagac cgacctgagc
     1801 ggcgccctga acgagcttca agacgaggct gcccgcctga gctggctggc caccaccggc
     1861 gtaccctgcg ccgctgtgtt ggatgttgtg accgaagccg gccgggactg gctgctgctg
     1921 ggcgaggtcc ctggccagga tctgctgagc agccaccttg cccccgctga gaaggtttct
     1981 atcatggccg atgcaatgcg gcgcctgcac accctggacc ccgctacctg ccccttcgac
     2041 caccaggcta agcatcggat cgagcgtgct cggacccgca tggaggccgg cctggtggac
     2101 caggacgacc tggacgagga gcatcagggc ctggcccccg ctgaactgtt cgcccgactg
     2161 aaagcccgca tgccggacgg tgaggacctg gttgtcacac acggagatgc ctgcctccct
     2221 aacatcatgg tcgagaatgg ccgcttctcc ggcttcatcg actgcggtcg cctaggagtt
     2281 gccgaccgct accaggacat cgccctggcc acccgcgaca tcgctgagga gcttggcggc
     2341 gagtgggccg accgcttctt agtcttgtac ggcatcgcag ctcccgacag ccagcgcatc
     2401 gccttctacc gcttgctcga cgagttcttt taatgatcta gaaccggtca tggccgcaat
     2461 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgttcg aactagatgc
     2521 tgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2581 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2641 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3241 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gcggccgcaa
     3601 atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc
     3661 tgcctatttc gttcgtccat agtggcctga ctccccgtcg tgtagatcac tacgattcgt
     3721 gagggcttac catcaggccc cagcgcagca atgatgccgc gagagccgcg ttcaccggcc
     3781 cccgatttgt cagcaatgaa ccagccagca gggagggccg agcgaagaag tggtcctgct
     3841 actttgtccg cctccatcca gtctatgagc tgctgtcgtg atgctagagt aagaagttcg
     3901 ccagtgagta gtttccgaag agttgtggcc attgctactg gcatcgtggt atcacgctcg
     3961 tcgttcggta tggcttcgtt caactctggt tcccagcggt caagccgggt cacatgatca
     4021 cccatattat gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt cagaagtaag
     4081 ttggccgcgg tgttgtcgct catggtaatg gcagcactac acaattctct taccgtcatg
     4141 ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag
     4201 tgtatacggc gaccaagctg ctcttgcccg gcgtctatac gggacaacac cgcgccacat
     4261 agcagtactt tgaaagtgct catcatcggg aatcgttctt cggggcggaa agactcaagg
     4321 atcttgccgc tattgagatc cagttcgata tagcccactc ttgcacccag ttgatcttca
     4381 gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca
     4441 aagaagggaa tgagtgcgac acgaaaatgt tggatgctca tactcgtcct ttttcaatat
     4501 tattgaagca tttatcaggg ttactagtac gtctctcaag gataagtaag taatattaag
     4561 gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac atctgtgtgt
     4621 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
     4681 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctct
//
