LOCUS       62056_20210        5672 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        109..140
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             173..1822
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1869..1990)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2184..2541
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2572..3363
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3391..3439
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3766..4354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4557..5414)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5581..5672
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg ctgcctcgat catgttatgc atattcctgt aagtgcatgt
       61 tatgcatatt cctgtaagtg gcgcgtagat ctgcagaagc ttagacacta gagggtatat
      121 aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga
      181 tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg
      241 cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac
      301 cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct
      361 ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga
      421 gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc
      481 cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc
      541 caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct
      601 accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag
      661 catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc
      721 cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg
      781 attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg
      841 cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt
      901 tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt
      961 gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca
     1021 atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa
     1081 gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt
     1141 aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac
     1201 agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg
     1261 caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg
     1321 tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa
     1381 caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat
     1441 cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa
     1501 atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa
     1561 catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc
     1621 agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc
     1681 cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc
     1741 taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa
     1801 gaagggcggc aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc
     1861 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     1921 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1981 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     2041 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtt
     2101 tgcgtattgg gcgctcttcc gctgatctgc gcagcaccat ggcctgaaat aacctctgaa
     2161 agaggaactt ggttagctac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     2221 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2281 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2341 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     2401 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     2461 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     2521 ggaggcctag gcttttgcaa aaagctcgat tcttctgaca ctagcgccac catgatcgaa
     2581 caagacggcc tccatgctgg cagtcccgca gcttgggtcg aacgcttgtt cgggtacgac
     2641 tgggcccagc agaccatcgg atgtagcgat gcggccgtgt tccgtctaag cgctcaaggc
     2701 cggcccgtgc tgttcgtgaa gaccgacctg agcggcgccc tgaacgagct tcaagacgag
     2761 gctgcccgcc tgagctggct ggccaccacc ggcgtaccct gcgccgctgt gttggatgtt
     2821 gtgaccgaag ccggccggga ctggctgctg ctgggcgagg tccctggcca ggatctgctg
     2881 agcagccacc ttgcccccgc tgagaaggtt tctatcatgg ccgatgcaat gcggcgcctg
     2941 cacaccctgg accccgctac ctgccccttc gaccaccagg ctaagcatcg gatcgagcgt
     3001 gctcggaccc gcatggaggc cggcctggtg gaccaggacg acctggacga ggagcatcag
     3061 ggcctggccc ccgctgaact gttcgcccga ctgaaagccc gcatgccgga cggtgaggac
     3121 ctggttgtca cacacggaga tgcctgcctc cctaacatca tggtcgagaa tggccgcttc
     3181 tccggcttca tcgactgcgg tcgcctagga gttgccgacc gctaccagga catcgccctg
     3241 gccacccgcg acatcgctga ggagcttggc ggcgagtggg ccgaccgctt cttagtcttg
     3301 tacggcatcg cagctcccga cagccagcgc atcgccttct accgcttgct cgacgagttc
     3361 ttttaatgat ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg
     3421 tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc
     3481 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     3541 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     3601 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3661 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3721 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3781 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3841 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3901 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3961 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4021 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4081 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4141 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4201 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4261 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4321 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4381 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4441 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4501 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
     4561 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
     4621 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
     4681 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
     4741 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
     4801 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
     4861 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
     4921 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
     4981 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
     5041 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
     5101 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
     5161 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
     5221 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
     5281 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
     5341 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
     5401 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
     5461 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
     5521 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     5581 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     5641 cagtgcaagt gcaggtgcca gaacatttct ct
//
