LOCUS       40924_41325        8126 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSTAR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Zeng Q, Tan YH, Hong W.
  TITLE     A single plasmid vector (pSTAR) mediating efficient 
            tetracycline-induced gene expression
  JOURNAL   Anal. Biochem. 259 (2), 187-194 (1998)
  PUBMED    9618196
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Zeng Q, Tan YH, Hong W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-1998) Membrane Biology Laboratory, Institute of 
            Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, 
            Singapore
REFERENCE   3  (bases 1 to 8126)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8126)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal. 
            Biochem."; date: "1998"; volume: "259"; issue: "2"; pages: "187-194"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-FEB-1998) Membrane Biology Laboratory, Institute of Molecular 
            and Cell Biology, 30 Medical Drive, Singapore 117609, Singapore"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    14..284
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        317..354
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    complement(482..616)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1187..1321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1339..2340)
                     /codon_start=1
                     /label=rtTA
                     /note="tetracycline-controlled transactivator, comprising a
                     fusion of the reverse tetracycline repressor rTetR with the
                     C-terminal activation domain of herpes simplex virus VP16"
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIE
                     GLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHAD
                     ALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        complement(2468..2671)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2672..3051)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3155..3484
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3841..4632
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     intron          5056..5121
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5251..5271
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    5696..5830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5931..6035
                     /label=AmpR promoter
     CDS             6036..6893
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7067..7655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
       61 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
      121 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
      181 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      241 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagc
      301 tcggtacccg ggtcgagtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      361 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      421 cgggaccgat ccagcctccg cggccccgaa ttcgagctcg gtacccgggg atcctctaga
      481 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
      541 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
      601 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
      661 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
      721 tcctgcaagc ctcgtcgtct ggccggacca cgctatctgt gcaaggtccc cggacgcgcg
      781 ctccatgagc agagcgcccg ccgccgaggc aagactcggg cggcgccctg cccgtcccac
      841 caggtcaaca ggcggtaacc ggcctcttca tcgggaatgc gcgcgacctt cagcatcgcc
      901 ggcatgtccc ctggcggacg ggaagtatca gctcgaccaa gctgttaccg cctgttgacc
      961 tggtgggacg ggcagggcgc cgcccgagtc ttgcctcggc ggcgggcgct ctgctcatgg
     1021 agcgcgcgtc cggggacctt gcacagatag cgtggtccgg ccagacgacg aggcttgcag
     1081 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     1141 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     1201 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1261 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     1321 cgatcctcgc gccccctacc caccgtactc gtcaattcca agggcatcgg taaacatctg
     1381 ctcaaactcg aagtcggcca tatccagagc gccgtagggg gcggagtcgt ggggggtaaa
     1441 tcccggaccc ggggaatccc cgtcccccaa catgtccaga tcgaaatcgt ctagcgcgtc
     1501 ggcatgcgcc atcgccacgt cctcgccgtc taagtggagc tcgtccccca ggctgacatc
     1561 ggtcgggggg gccgtcgaca gtctgcgcgt gtgtcccgcg gggagaaagg acaggcgcgg
     1621 agccgccagc cccgcctctt cgggggcgtc gtcgtccggg agatcgagca ggccctcgat
     1681 ggtagacccg taattgtttt tcgtacgcgc gcggctgtac gcggacccac tttcacattt
     1741 aagttgtttt tctaatccgc atatgatcaa ttcaaggccg aataagaagg ctggctctgc
     1801 accttggtga tcaaataatt cgatagcttg tcgtaataat ggcggcatac tatcagtagt
     1861 aggtgtttcc ctttcttctt tagcgacttg atgctcttga tcttccaata cgcaacctaa
     1921 agtaaaatgc cccacagcgc tgagtgcata taacgcgttc tctagtgaaa aaccttgttg
     1981 gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag gccgtgtatc
     2041 tgaatgtact tttgctccat tgcgatgact tagtaaagca catctaaaac ttttagcgtt
     2101 attgcgtaaa aaatcttgcc agctttcccc ttttaaaggg caaaagtgag tatggtgcct
     2161 atctaacatc tcaatggcta aggcgtcgag caaagcccgc ttatttttta catgccaata
     2221 cagtgtaggc tgctctacac caagcttctg ggcgagttta cgggttgtta aaccttcgat
     2281 tccgacctca ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctaga
     2341 gggtctgggt ctctttggca tggtgaatta attcggggcc gcggaggctg gatcggtccc
     2401 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
     2461 ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     2521 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     2581 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     2641 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     2701 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     2761 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     2821 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     2881 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     2941 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     3001 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacatggcg
     3061 gtaatgttgg acatgagcca atataaatgt acatattatg atatggatac aacgtatgca
     3121 atgggccaag ctcctcgaga tccggctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3181 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3241 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3301 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3361 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3421 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3481 caaaaagctt cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac
     3541 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
     3601 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     3661 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     3721 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     3781 aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc
     3841 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3901 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3961 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4021 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4081 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4141 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4201 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4261 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4321 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     4381 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4441 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4501 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4561 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4621 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     4681 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4741 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4801 cccaccccgg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc
     4861 tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc
     4921 cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcccgg
     4981 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     5041 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     5101 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     5161 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     5221 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     5281 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     5341 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     5401 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     5461 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
     5521 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
     5581 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5641 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5701 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5761 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5821 tgtctggatc tcgaggacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5881 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5941 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6001 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6061 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6121 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6181 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6241 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6301 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6361 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6421 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6481 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6541 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6601 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6661 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6721 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6781 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6841 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6901 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6961 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7021 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7081 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7141 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7201 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7261 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7321 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7381 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7441 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7501 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7561 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7621 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7681 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7741 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7801 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7861 tccccgcgcg ttggccgatt cattaatgca gctgaacggt ctggttatag gtacattgag
     7921 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     7981 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgccaagc
     8041 ttggtcgagc tgatacttcc cgtccgccag gggacatgcc ggcgatgctg aaggtcgcgc
     8101 gcattcccga tgaagaggcc ggttac
//
