LOCUS       40924_41320       11849 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSTA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11849)
  AUTHORS   Baka E, Varga S, Hobel C, Knispel RW, Fekete C, Ivanics M, Kriszt B,
            Nagy I, Kukolya J.
  TITLE     The first transformation method for the thermo-acidophilic archaeon 
            Thermoplasma acidophilum
  JOURNAL   J. Microbiol. Methods 95 (2), 145-148 (2013)
  PUBMED    23978635
REFERENCE   2  (bases 1 to 11849)
  AUTHORS   Baka E, Kukolya J, Nagy I, Varga S, Knispel R.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2013) Department of Microbiology, Central 
            Environmental and Food Science Research Institute, Herman Otto Road 
            15, Budapest 1022, Hungary
REFERENCE   3  (bases 1 to 11849)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11849)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2013"; volume: "95"; issue: "2"; pages: 
            "145-148"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-FEB-2013) Department of Microbiology, Central Environmental and 
            Food Science Research Institute, Herman Otto Road 15, Budapest 1022,
            Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape Plasmid Editor v. 2.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..6100
                     /label=pTA1 plasmid NcoI fragment
                     /note="pTA1 plasmid NcoI fragment"
     misc_feature    1..163
                     /note="derived from pTA1 plasmid ORF13 from T. acidophilum
                     of INSD accession AB190359"
     CDS             complement(122..457)
                     /codon_start=1
                     /product="hypothetical membrane protein"
                     /label=hypothetical membrane protein
                     /note="derived from pTA1 plasmid ORF14 from T. acidophilum
                     of INSD accession AB190359"
                     /protein_id="AGL45415.1"
                     /translation="MCISMICLCRPVLVSCTCCFPGVCVRILGISLAPCLRSYLGRPLQ
                     YPFSYLSYYYVLVVKNIGFVVCRFWRPALVADPALRFRLLPFFITLDNVRSAPLFVRGH
                     IFFGCLI"
     CDS             500..808
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="derived from pTA1 plasmid ORF15 from T. acidophilum
                     of INSD accession AB190359"
                     /protein_id="AGL45416.1"
                     /translation="MNSETYILIFIDNLPRKIPTWIAESKNIKEFEHFSLKKGTEKAML
                     ISNQRLEKWIPVSKTKRIKKENETALEWAIKELNNPEHRNEATAKIRKLINSEEGEQ"
     CDS             805..1044
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="derived from pTA1 plasmid ORF16 from T. acidophilum
                     of INSD accession AB190359"
                     /protein_id="AGL45417.1"
                     /translation="MTDLKQLLKDHHPEIKEMPSHKEAIEILLQDLENIYKRMRILYYC
                     RNCKKIINPGEEYLHFQHILEDAIILQEPPPKRN"
     CDS             1004..1381
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="derived from pTA1 plasmid ORF17 from T. acidophilum
                     of INSD accession AB190359"
                     /protein_id="AGL45418.1"
                     /translation="MPLYYKNPHQKEIDGPLIFSPTIITKSNKKNTFNKSMPYQGMKQT
                     INPEEILHLARIGEIGTIDSYKGQPLIVNPLNDRPISIQYDPHTDSFFATIGSRNIYLT
                     DKQASRLIRDLTKYLDQITKI"
     CDS             1702..2115
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="derived from pTA1 plasmid ORF18 from T. acidophilum
                     of INSD accession AB190359"
                     /protein_id="AGL45419.1"
                     /translation="MPVINANISDEAYTILKEITEQTDGNKSKALTKIILAFKECMENH
                     QQTASEEHEEDQGSTEDIEQIKKFLQDQTEEAPERYQITKGELYQKFLEYCKKSSHPEI
                     PKPTFYSIIKRNIKIQDNDNARPRVYKGIKWKE"
     CDS             2553..3638
                     /codon_start=1
                     /gene="Cdc6"
                     /product="cell division control protein 6-like protein"
                     /label=Cdc6
                     /note="derived from pTA1 plasmid ORF1 from T. acidophilum
                     of INSD accession AB190359; putative replication initiator"
                     /protein_id="AGL45420.1"
                     /translation="MISDKNNLDELTRKTIETLKDNGNNLKDPSVFDLNYQPKKIFIRK
                     EINAILEQLGQYALWKIPKHIALIGPKGAGKTITIKTIAESLQRTQGLENIYINARETQ
                     TSYKIYQEITKHYTKGPDLSEMRAKALKRLTDHTLLIIDEVDFLKDQDILYTVTRETKT
                     TLILLTQKLSWIKNLKDESVASSLQPQLITFNQYTPQELSEILTMRAEEGLYQFDPGSI
                     NLISALVARNYRGDTRIAIKTLERIGYRNQWNEEEIYKALEEAYNELEGTILRGLSPRD
                     LEILLIISKTPESNKAYLIAKQSTYARNISRPTFLQALNHLQNLDLIMLTKKRVGKSNT
                     QEAEILLNNKDLIEKIASEKL"
     gene            2553..3638
                     /gene="Cdc6"
                     /label=Cdc6
     CDS             complement(4196..5080)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="derived from pTA1 plasmid ORF2 from T. acidophilum
                     of INSD accession AB190359"
                     /protein_id="AGL45421.1"
                     /translation="MRSGPCGVLPRIFRISMRAWLIKFSSIFSFSSIHDGDLRRFAMIN
                     DFLAWAVANLKPYQFIDVRNAYSERLDVPIFTLDYAGFSAYMDAVVGMNWDVRYEDEVM
                     AVLKNGPASVSDIVDRITSKYPVSRKEASDKVWLVLGRFRDNDMGWADCQGHVLKLRHI
                     DMDLKHDEGGLWFLSGSNMSVLHDWMEGQVVRVLRDYHVEFAVPPEYTRRRDADIVLHG
                     FKIEIETGLKKDSSALVDRISSYDGPVVVVVPNQAVKSRYARILSGFQNVNVLTLLEFI
                     YDLKREYIKSVFK"
     CDS             5001..5603
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="derived from pTA1 plasmid ORF3 from T. acidophilum
                     of INSD accession AB190359"
                     /protein_id="AGL45422.1"
                     /translation="MLENLISQALIDIRKILGSTPQGPERTDLTHHIVNHCGTHTGQPV
                     DLIDGQDNRTAPRVYHPPDLTEEKLGIEGFCTLIKAGEIEDVVIIDAKGEIDHRIGRYK
                     TQVSLYNGPGLTLDPGSIRPAGPHDIQKIIIGRRNHDRRMQERRDLRSGYTLDVNGKGN
                     IPRINEGITIKKEGIRDPISNPNNTKRKMQIRIIGLK"
     CDS             6112..6129
                     /label=6xHis
                     /note="6xHis affinity tag"
     promoter        6243..6261
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6262..6286
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             6395..6439
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     terminator      6491..6538
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6771..7583)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(7584..7675)
                     /label=AmpR promoter
     rep_origin      complement(7691..8440)
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     misc_feature    8446..8852
                     /label=promoter Ta1137/1138
                     /note="promoter Ta1137/1138"
     CDS             8856..10781
                     /codon_start=1
                     /gene="gyrB"
                     /product="gyrase B"
                     /label=gyrB
                     /note="Ta10555"
                     /protein_id="AGL45423.1"
                     /translation="MTEDNYDSSQIQILEGLKAVRKVPGMYIGSTDTRGLHHLVYEVVD
                     NSVDESVAGYCSRIYVVMGSDGSITVEDDGRGIPVDIHPKYNRPGLEIVLTELHSGAKF
                     DKKVYKLTGGLHGVGLHVVNALSKKLIAVVKRDGKIYYDIFEQGIPVSGLKTASDVSEI
                     EKLGIKIQFPEHGAIIKFYPDPDIFETTEFSYETILARLTDLSYLNPQLTITFLDEASG
                     RKDILHHEGGLIELVRHLSEGKEALMEPLYLKEEVDSHMVEFSLLYTTDVQETLMSFVN
                     NISTPEGGTHVAGFHQGLSRAIQDYARSNNKIKGVEDITGDDVKEGVIAVLHVKMQNPQ
                     FEGQTKSKLGNSSVRGIVQSVTAKFMKTFMETNPHVADVIIGRVLSAAAAREASRKAKE
                     LVRRKSALEGGGLPGKLADCSSNDPSNSELYIVEGDSAGGSAKQARNRQYQAVLPLRGK
                     ILNVEKASDMKVVENEIIHDLVVAIGTGVKEDLNPKKLRYGKIIIMTDADVDGAHIRTL
                     LLTFFFRYARSLIENGNVFFAEPPLYRIQKGQNVRYVYSDEEKEQVSREFGPNAIIQRF
                     KGLGEMNPEQLWETTMNPKTRKLVQVTIEDAEEAERIFTILMGEKVEPRRKFIEENAVY
                     AENIDL"
     gene            8856..10781
                     /gene="gyrB"
                     /label=gyrB
     misc_feature    8856..9022
                     /gene="gyrB"
                     /note="Ta1055; derived from INSD accession AL445066"
     misc_feature    9023..9993
                     /gene="gyrB"
                     /label=derived from INSD accession AB206999
                     /note="derived from INSD accession AB206999"
     misc_feature    9994..10781
                     /gene="gyrB"
                     /note="Ta1055; derived from INSD accession AL445066"
     promoter        complement(11639..11716)
                     /label=lacI promoter
     promoter        11762..11780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    11781..11805
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             11820..11842
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tggaacacca aaaaagcagg aaaacaggga tttatccata tcaaatttta aattccagga
       61 agacacaaag acgccactat acgatatatt ccatccccca aggaaggata acacgagaaa
      121 ttcagatcag gcaaccaaaa aaaatatgac ctcgaacgaa tagaggagca gaacgaacat
      181 tatccaaagt aataaaaaac ggcaaaagcc gaaaacggag ggccggatcc gccaccaggg
      241 caggcctcca aaaacggcaa acaacgaacc caatattttt aacaaccaag acataatagt
      301 atgataaata tgaaaaggga tattgcaatg gccgacccaa ataagaacgg agacaaggag
      361 caagagatat gcccaatatg cggacacata cacctggaaa acaacatgtg catgacacca
      421 atacaggacg gcataggcat atcatggata tgcacatgca cagaggaaca ggaaacccat
      481 acaacaggag agtggaatta tgaacagtga aacctacatc ctcatcttca ttgacaacct
      541 acccaggaag atcccgacat ggatcgctga aagcaaaaat ataaaagaat tcgaacattt
      601 ttcactcaaa aaggggacgg aaaaggccat gttgataagc aaccagagac tggaaaaatg
      661 gataccagta tcaaagacga aaaggataaa gaaggaaaac gaaacagcgt tagaatgggc
      721 aataaaggaa ctaaacaacc cagagcatcg gaatgaggca acagcaaaga tccgaaagct
      781 aatcaacagc gaggagggag aacaatgacg gatctaaagc aactcctcaa agatcaccat
      841 ccagaaataa aagagatgcc atcacataag gaggccatag agatcctact acaggacctc
      901 gaaaacatct ataaacgcat gagaatccta tactactgcc ggaattgcaa gaaaataatc
      961 aatcccggcg aagagtatct tcatttccag catatattag aggatgccat tatattacaa
     1021 gaacccccac caaaaagaaa ttgatggacc attaattttt tccccaacca tcataaccaa
     1081 aagtaataaa aagaatactt ttaataaaag tatgccttac caaggcatga aacaaaccat
     1141 aaatccagag gagatcctcc atctggcaag gatcggagaa ataggaacga tagacagcta
     1201 caagggccaa ccactaatcg taaacccatt gaatgatagg cccatatcaa tccaatacga
     1261 cccgcataca gacagctttt tcgcaaccat aggaagcaga aatatatacc ttacagacaa
     1321 acaggccagc aggttaataa gagatctgac aaaatattta gaccagataa caaagatata
     1381 atatgccata ctattattat atctatgaaa aataaaacca ccatcaagat atccccagaa
     1441 acgcagggcc ttctaaacct tttgaaagca cgggtaaatg aaacccggac catcgggaac
     1501 aaactaaccc atgatgagat attaaagatc ctgatcgagg acctactcaa atcagggaaa
     1561 tatctcgaac ttttcaaaga caagtaaaat catcatccca aaagaccaaa caacaccatt
     1621 cttattatta ttaataagaa caactcttaa tattaataag aaaccaacaa agattaatat
     1681 tattattaag aattaacaca catgccagtc ataaacgcaa acatatcgga cgaagcttat
     1741 acgatcctga aggaaataac agaacaaaca gacggaaata aatcgaaggc cctaacgaag
     1801 atcatcctgg cattcaaaga atgcatggaa aaccatcaac aaacagccag cgaggaacac
     1861 gaagaagacc aaggaagcac agaagatatc gaacaaataa aaaaattcct ccaagaccaa
     1921 acagaagaag caccagaaag atatcaaatc acaaagggag aactatacca gaaattcctg
     1981 gaatactgca aaaagtcatc gcatccggaa atcccaaagc ccacattcta ctccatcata
     2041 aagagaaata tcaagatcca agataacgac aacgcaagac caagagtata taaagggata
     2101 aaatggaaag aatgaagata cactttatcg caaaaacgaa ataaaattaa tttttttcaa
     2161 acggaaccaa gaaactacgg cttttctttt aaaaaaaaga tccacagggg ggacatttaa
     2221 cgacaccatc ccagaaaaag aaccccccac ccacaaaacc atccgatcga tcccatagaa
     2281 tcaagaaagc aaaaccttat atacatctct ttttgattaa tatattaatt ctgcaagctg
     2341 tattatgcta aaacagaaat taatataata aataaaaaat aattttacga tcgcactata
     2401 cactattttc cccattttgt ggtaaaatgt cccccctctc tggcattttt gaaagccgaa
     2461 caagcattgc gtaaggaaaa tctacaaaat caacaatttt gaaattaaac acgtaaaaga
     2521 aattaaatac aacaatgaca ttaatccaat caatgataag cgacaaaaac aacctggacg
     2581 aactgacgag gaagacgatc gaaacgctga aggacaacgg gaacaacctt aaggacccga
     2641 gcgtattcga tctgaactac cagcccaaga agatatttat tagaaaagag atcaacgcta
     2701 tcctggaaca actgggccag tatgcattat ggaagatccc aaagcatata gccctgatag
     2761 gcccaaaggg agcaggaaag accataacga tcaagaccat cgcagaatca ttgcagagaa
     2821 cgcaggggct tgaaaacatc tacatcaacg cccgggaaac gcagacatca tacaagatat
     2881 atcaggagat aacaaagcac tacaccaaag ggccggatct atccgagatg agggcaaaag
     2941 cattaaagcg attaacggac cacaccctac tgatcatcga tgaagtcgat tttctgaagg
     3001 accaagacat cctttacaca gtaacaagag agaccaaaac aaccctcata ctattgaccc
     3061 agaaactatc atggatcaag aacctgaaag acgaaagcgt agccagcagt ttacaacccc
     3121 agctcatcac attcaaccaa tacacccccc aggaattatc ggagatcctg acaatgagag
     3181 cggaggaagg cctataccaa ttcgatccag gaagcataaa cctaatatcg gcactggtag
     3241 cacgaaacta caggggagat actcgaatag caataaagac cctggaaagg atcggataca
     3301 ggaaccagtg gaacgaagag gagatctata aagcacttga agaagcgtat aacgaactgg
     3361 aaggcaccat actgagaggc ctatcaccca gagacctgga aatcctccta atcatctcca
     3421 agacaccgga aagcaataaa gcatacctga tcgcaaagca aagcacgtat gccaggaaca
     3481 tatccaggcc gacattcctc caggccctaa accacctcca aaacctggat ctgatcatgc
     3541 tgacgaagaa gagagtagga aaatcgaaca cccaggaggc agagatcctt ttgaacaata
     3601 aggatctcat agaaaaaatc gcaagtgaaa agctataatc ccccgatacc gaaataaagg
     3661 gggataccgc attacacggc ataaatcatc attccacaca caaccacgaa tacggaaaac
     3721 ccaaattcca attttatata tgataaattt agaaatccaa taaggcaagg agaacccaca
     3781 aaacgcccaa tatgccgtaa aagaccctcg ataacaacca gtatcgggcc tgaaaagaga
     3841 aagagaaagg ggcagtatat atttaaaact ctttccagta tcgcttatgc gttgtctgat
     3901 gcataagcgg gggaaatttg cgacgggcct gtctaaacgc cgtcttgctt cggtagagga
     3961 cagctttccg ataacgctca tttcctcgct gaaaaaaatt cacccagacc ctcttttttc
     4021 cagcttcgtt attcgctatc ggaattcagg ctaccgcctg aaccgccgcc ctctaccgaa
     4081 gcgacggcag ggatcaccct gtttagccag gctaaacttt ttatgagcga agcgaataaa
     4141 aagccgtcgc aaatttccac actggaaaga gaaagagaaa atgagaaaaa gagaatcact
     4201 taaaaacaga tttaatgtat tctcttttca gatcgtaaat aaattccaga agagtgagga
     4261 cattcacatt ctgaaaccct gacaggatcc gagcataccg ggacttgacg gcctgattag
     4321 gcacaaccac gacaacaggg ccatcatacg aggatatccg atccacaagg gccgaactgt
     4381 cctttttcag gcccgtctct atctcaattt tgaagccatg caggactata tccgcatccc
     4441 tacgcctggt atattcagga ggcacggcaa actcaacatg ataatcccgc agaaccctga
     4501 caacctggcc ctccatccag tcatgcagaa cggacatatt ggatccagac aggaaccaga
     4561 gaccgccctc atcatgttta agatccatat cgatatgcct caacttcaaa acatgaccct
     4621 ggcaatcggc ccaccccata tcgttatcac ggaatcggcc cagaacgagc cagaccttat
     4681 cgctggcctc tttcctcgat accggatatt ttgaagttat gcgatccaca atatccgaca
     4741 cggaagccgg gccattcttc aataccgcca tcacctcatc ctcatacctc acatcccaat
     4801 tcattccaac caccgcatcc atgtaagccg aaaaaccggc atagtcaaga gtgaatatgg
     4861 gcacatccaa ccgctcacta taagcattcc tcacatcgat gaactgatag ggcttcaaat
     4921 tcgccacggc ccaggcaagg aaatcgttga tcatcgcaaa gcgacggaga tccccatcat
     4981 ggatagacga gaagctgaat atgctcgaaa acttgattag ccaggccctc attgatatcc
     5041 gaaaaattct gggaagcact ccacagggcc ccgaacgcac ggatctgacg catcacatcg
     5101 taaaccactg tggaacgcac acgggccaac ctgtggatct catcgatggc caggacaaca
     5161 ggaccgcccc cagagtatat catccgccag acctgacgga ggaaaaactc ggcatagaag
     5221 gtttttgcac gctcattaag gccggagaaa tcgaagacgt agttatcatc gatgcgaaag
     5281 gggaaatcga tcacaggata gggagatata agacgcaggt tagcctctat aacggcccag
     5341 gcctcactct ggatcctgga agcatccgcc ctgcgggacc tcacgatatc cagaagatca
     5401 tcataggacg acgcaaccat gaccgcagaa tgcaggagag aagagatctg agaagcggtt
     5461 ataccctgga tgtcaacggg aaaggcaaca tcccaagaat caacgaaggc atcacgatca
     5521 agaaggaagg gattagggac cccatcagca acccgaataa tacgaaaagg aagatgcaga
     5581 taagaatcat tgggcttaaa tgagaagact atcttatatc catcaaaggc catgagaaga
     5641 taattcatca attgagactt accggacccg gaggacccgc atatcaggac attcctcgta
     5701 gcacgggatt ttatcaggcc cacaagatcc gcatccggca tttcatcgat cgaataatcg
     5761 agatcgaaca atgcagagaa gggcaaccgc ttaaccatac ccataaaccc aaaatatgaa
     5821 tacttttaat aaaagtatgc cttcactatc caaatgaaga acagaaggca gttagaagac
     5881 cgattcatac aggaaatgac caggttagga tacctatgca tggaaggcac cgctgacggc
     5941 aaaatggaac ccagccacga agcgatacta tccaacggca tcggactttt acgggacatg
     6001 ggacattgaa gcagtgaagg ggcccatcac catccgcata caggtaacat caggatcata
     6061 cgaatcattg aggaagcaca agaggaagat agagaagcca tgggcagcag ccatcaccat
     6121 catcaccaca gccaggatcc gaattcgagc tcggcgcgcc tgcaggtcga caagcttgcg
     6181 gccgcataat gcttaagtcg aacagaaagt aatcgtattg tacacggccg cataatcgaa
     6241 attaatacga ctcactatag gggaattgtg agcggataac aattccccat cttagtatat
     6301 tagttaagta taagaaggag atatacatat ggcagatctc aattggatat cggccggcca
     6361 cgcgatcgct gacgtcggta ccctcgagtc tggtaaagaa accgctgctg cgaaatttga
     6421 acgccagcac atggactcgt ctactagcgc agcttaatta acctaggctg ctgccaccgc
     6481 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     6541 gaaacctcag gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt
     6601 aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga cgacaagctg
     6661 acgaccgggt ctccgcaagt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6721 tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta gaaaaactca
     6781 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     6841 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     6901 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     6961 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     7021 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     7081 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     7141 cgaaatacgc ggtcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     7201 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     7261 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     7321 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     7381 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     7441 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     7501 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac
     7561 gtttcccgtt gaatatggct catactcttc ctttttcaat attattgaag catttatcag
     7621 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggc
     7681 atgcagcgct cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     7741 cgagcggtgt cagctcactc aaaagcggta atacggttat ccacagaatc aggggataaa
     7801 gccggaaaga acatgtgagc aaaaagcaaa gcaccggaag aagccaacgc cgcaggcgtt
     7861 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gccagaggtg
     7921 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7981 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8041 cgtggcgctt tctcatagct cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc
     8101 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8161 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccattgg
     8221 taactgattt agaggacttt gtcttgaagt tatgcacctg ttaaggctaa actgaaagaa
     8281 cagattttgg tgagtgcggt cctccaaccc acttaccttg gttcaaagag ttggtagctc
     8341 agcgaacctt gagaaaacca ccgttggtag cggtggtttt tctttattta tgagatgatg
     8401 aatcaatcgg tctatcaagt caacgaacag ctattccgtt actctatagc gaaacctcta
     8461 atcaaatctc catataaaaa actgatcggt tgcgatattc tgcatccccg cacaaatgaa
     8521 tgatggatta atcagtgcta ggaataaaga aggccagatt ggacctgatt taaatttgca
     8581 aacgtaggct tatttgcctg acataatata aataatgaag ttcaccattc aatgcctgtt
     8641 gataagggcg aaattgtatc tggatgaata tcaatacagg atactgaaac cagacgctga
     8701 gggatattat gggaaagctg aggtttcgct gatacacggc aatgatggtt tttacattta
     8761 tatagaagca cctgatacag gatcgctgag aacttcactg tcctcaatcg ctagattaat
     8821 tcatgtaata tatggtgtgg agggattgat cacatatgac cgaagacaat tacgactctt
     8881 cacagataca gatacttgaa gggttgaagg ctgttagaaa agtaccgggc atgtacatag
     8941 gctccaccga tacaaggggc ctgcaccacc tggtctatga ggttgtggac aacagcgttg
     9001 atgaatcagt tgcgggatac tgcagcagga tatacgttgt gatggggagc gatggatcca
     9061 taactgtgga ggatgacggt aggggtatac ctgtcgatat acatcccaag tacaacaggc
     9121 caggactgga gatcgttctt acggaactcc acagcggtgc gaagtttgat aaaaaggttt
     9181 acaagctaac agggggcttg catggggtgg gccttcatgt cgtgaacgcg ctttcaaaaa
     9241 agcttatagc cgtggtgaag agagacggca agatatacta cgacatattc gaacagggta
     9301 taccggtgtc gggcctgaaa actgccagcg atgtttctga aattgaaaaa cttggaataa
     9361 agattcagtt tccagagcac ggtgccataa taaaattcta tccggatccc gacatatttg
     9421 agacgaccga attttcatac gaaaccatac tggcaaggtt gaccgatctc tcctatctga
     9481 atccgcagct cacgataaca ttcttggacg aggcctccgg aaggaaggat atactccatc
     9541 atgagggcgg tctcatcgag ctggtcaggc acctgtccga gggaaaggag gcgttgatgg
     9601 aaccgctgta cctcaaggaa gaggtagaca gccacatggt agagttctcg ctattgtaca
     9661 caacggatgt tcaggaaacg ctaatgtcat tcgtgaacaa cataagcacg ccggaggggg
     9721 gtactcatgt ggctggcttt caccagggcc tttcgcgtgc aatacaggat tacgccagat
     9781 caaacaacaa gataaagggt gttgaggaca taactggcga tgatgtgaag gaaggtgtca
     9841 tcgcagttct tcacgttaag atgcagaatc cgcagttcga aggccagaca aaatcaaagc
     9901 tcggaaacag cagcgtgagg ggtatcgttc agtccgtaac agcgaagttc atgaagacgt
     9961 tcatggagac gaatccgcac gttgcagatg ttataatagg aagggttcta tccgctgcgg
    10021 ctgccaggga ggcgtcaagg aaggccaagg agcttgtgag gagaaaatct gctcttgagg
    10081 gcggcggtct tccaggaaag cttgccgact gttcatcgaa cgatccgtca aacagcgagc
    10141 tttacatagt tgagggagac tctgctggag gatcggccaa acaggcgagg aacaggcagt
    10201 atcaggctgt acttcctctc agaggaaaga tactcaacgt tgagaaagct tcggacatga
    10261 aggttgtgga gaacgagatc attcatgacc tcgttgtcgc cataggtaca ggcgtcaagg
    10321 aagacctgaa tccgaagaag ctcaggtacg ggaagataat aatcatgacc gatgccgatg
    10381 tggacggcgc acatataaga acgctgctgt tgacattctt cttcaggtac gcaagatcgc
    10441 tgatagagaa cggaaacgtg ttctttgcag aaccaccgct gtacaggata cagaaggggc
    10501 agaacgtcag atacgtttac tccgatgagg agaaggaaca ggtaagccgt gagttcggac
    10561 cgaatgcaat aatacagagg ttcaagggtc ttggcgaaat gaatcccgag cagctgtggg
    10621 agacgaccat gaatcccaag actaggaagc tcgttcaggt aacaatagag gacgctgagg
    10681 aggcggagcg catattcaca atactcatgg gagagaaggt tgaaccaagg aggaagttca
    10741 tcgaagaaaa cgcggtttat gctgagaaca tagacctctg aaacggcggg atataacatg
    10801 agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg
    10861 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
    10921 tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc
    10981 agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
    11041 cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
    11101 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa
    11161 taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc
    11221 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
    11281 tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt
    11341 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
    11401 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
    11461 gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
    11521 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa
    11581 cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat
    11641 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
    11701 gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaaa
    11761 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt
    11821 ttgtttaact ttaataagga gatatacca
//
