LOCUS       V003035                 4598 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003035
VERSION     V003035
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Markerless gene replacement in Escherichia coli stimulated by a
            double-strand break in the chromosome
  JOURNAL   Nucleic Acids Res. 27 (22), 4409-4415 (1999)
   PUBMED   10536150
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center
            Hung. Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary
REFERENCE   3  (bases 1 to 4598)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4598)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1999"; volume: "27"; issue: "22"; pages:
            "4409-4415"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center Hung.
            Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..697)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        713..768
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             782..1474
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     promoter        1525..1543
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1551..1635
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     misc_feature    1588
                     /label="I-SceI cleavage site"
                     /note="I-SceI cleavage site"
     CDS             complement(1767..2714)
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     rep_origin      complement(2762..2984)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(3609..4466)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4467..4571)
                     /label="AmpR promoter"
ORIGIN
        1 atcgatgggt ggttaactcg acatcttggt taccgtgaag ttaccatcac ggaaaaaggt
       61 tatgctgctt ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca
      121 attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt
      181 gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt
      241 gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat
      301 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag
      361 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac
      421 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc
      481 cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga
      541 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct
      601 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc
      661 tgttaatcac tttactttta tctaatctag acatcattaa ttcctaattt ttgttgacac
      721 tctatcattg atagagttat tttaccactc cctatcagtg atagagaaaa gtgaaatgca
      781 tcaaaaaaac caggtaatga acctgggtcc gaactctaaa ctgctgaaag aatacaaatc
      841 ccagctgatc gaactgaaca tcgaacagtt cgaagcaggt atcggtctga tcctgggtga
      901 tgcttacatc cgttctcgtg atgaaggtaa aacctactgt atgcagttcg agtggaaaaa
      961 caaagcatac atggaccacg tatgtctgct gtacgatcag tgggtactgt ccccgccgca
     1021 caaaaaacaa cgtgttaacc acctgggtaa cctggtaatc acctggggcg cccagacttt
     1081 caaacaccaa gctttcaaca aactggctaa cctgttcatc gttaacaaca aaaaaaccat
     1141 cccgaacaac ctggttgaaa actacctgac cccgatgtct ctggcatact ggttcatgga
     1201 tgatggtggt aaatgggatt acaacaaaaa ctctaccaac aaatcgatcg tactgaacac
     1261 ccagtctttc actttcgaag aagtagaata cctggttaag ggtctgcgta acaaattcca
     1321 actgaactgt tacgtaaaaa tcaacaaaaa caaaccgatc atctacatcg attctatgtc
     1381 ttacctgatc ttctacaacc tgatcaaacc gtacctgatc ccgcagatga tgtacaaact
     1441 gccgaacact atctcctccg aaactttcct gaaataataa gtcgacccga attggtcgac
     1501 aagcttgatc tggcttatcg aaattaatac gactcactat agggagaccg gaattcgagc
     1561 tcggtacccg gggatccgct agggataaca gggtaatata gatcctctag agtcgacctg
     1621 caggcatgca agcttggcac tggctgatca gctagcccat gggtatggac agttttccct
     1681 ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat gcttcactga
     1741 tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat gttctctagt
     1801 gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcatg cttactttgc
     1861 atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg
     1921 tgtagtgttt ttcttagtcc gttacgtagg taggaatctg atgtaatggt tgttggtatt
     1981 ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta
     2041 tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat
     2101 ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc
     2161 cttttaaact catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta
     2221 atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa
     2281 atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat
     2341 ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg
     2401 cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac caaaggattc
     2461 ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac accataagca
     2521 ttttccctac tgatgttcat catctgaacg tattggttat aagtgaacga taccgtccgt
     2581 tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc
     2641 ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca
     2701 actaattcag acatacatct caattggtct aggtgatttt aatcactata ccaattgaga
     2761 tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat ctgtaaattc
     2821 tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga
     2881 cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa
     2941 taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt
     3001 ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa
     3061 aaccctaaag gcttaagtag caccctcgca agctcggttg cggccgcaat cgggcaaatc
     3121 gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca
     3181 ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc
     3241 ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct
     3301 cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt
     3361 tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga
     3421 ctggctaatg cacccagtaa ggcagcggta tcatcaacgg ggtctgacgc tcagtggaac
     3481 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3541 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3601 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3661 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3721 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3781 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3841 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3901 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3961 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4021 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4081 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4141 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4201 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4261 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4321 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4381 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4441 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4501 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4561 ggggttccgc gcacatttcc ccgaaaagtg ccacctgc
//
