LOCUS       40924_41270        4234 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Unidentified cloning vector pSSD3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Kato S, Saeki M.
  TITLE     Signal sequence detection vector pSSD3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Kato S, Saeki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-1997) Contact:Seishi Kato Research Institute of 
            National Rehabilitation Center for the Disabled, Department of 
            Rehabilitation Engineering; 4-1 Namiki, Tokorozawa, Saitama 
            359-8555, Japan
REFERENCE   3  (bases 1 to 4234)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4234)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-1997) Contact:Seishi Kato Research Institute of National 
            Rehabilitation Center for the Disabled, Department of Rehabilitation
            Engineering; 4-1 Namiki, Tokorozawa, Saitama 359-8555, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..346
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          385..481
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     525..541
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        549..567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    581..586
                     /note="SmaI site for cloning of a target cDNA fragment
                     encoding a putative signal sequence"
     misc_feature    588..593
                     /note="PmaCI site for cloning of a target cDNA fragment
                     encoding a putative signal sequence"
     misc_feature    595..600
                     /note="EcoRV site for cloning of a target cDNA fragment
                     encoding a putative signal sequence"
     CDS             complement(1028..1039)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(1490..1508)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(1510..1644)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1942..2530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2704..3561)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3562..3666)
                     /label=AmpR promoter
     rep_origin      complement(3693..4148)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4166..4182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
       61 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
      121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
      181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
      241 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
      301 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga
      361 actgaaaaac cagaaagtta actggtaagt ttagtctttt tgtcttttat ttcaggtccc
      421 ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc tttacttcta
      481 ggcctgtacg gaagtgttac ttctgctcta aaagctgctc gagtgtaaaa cgacggccag
      541 tacgtattta atacgactca ctatagggaa ttccacagat cccgggtcac gtgggatatc
      601 cctcctctcc tccagaagaa ttaaaatttc agtgtggcca aaagactctg aggccccgct
      661 ttaagattat tgggggagaa ttcaccacca tcgagaacca gccctggttt gcggccatct
      721 acaggaggca ccgggggggc tctgtcacct acgtgtgtgg aggcagcctc atcagccctt
      781 gctgggtgat cagcgccaca cactgcttca ttgattaccc aaagaaggag gactacatcg
      841 tctacctggg tcgctcaagg cttaactcca acacgcaagg ggagatgaag tttgaggtgg
      901 aaaacctcat cctacacaag gactacagcg ctgacacgct tgctcaccac aatgacattg
      961 ccttgctgaa gatccgttcc aaggagggca ggtgtgcgca gccatcccgg actatacaga
     1021 ccatctgcct gccctcgatg tataacgatc cccagtttgg cacaagctgt gagatcactg
     1081 gctttggaaa agagaattct accgactatc tctatccgga gcagctgaaa atgactgttg
     1141 tgaagctgat ttcccaccgg gagtgtcagc agccccacta ctacggctct gaagtcacca
     1201 ccaaaatgct gtgtgctgct gacccacagt ggaaaacaga ttcctgccag ggagactcag
     1261 ggggacccct cgtctgttcc ctccaaggcc gcatgacttt gactggaatt gtgagctggg
     1321 gccgtggatg tgccctgaag gacaagccag gcgtctacac gagagtctca cacttcttac
     1381 cctggatccg cagtcacacc aaggaagaga atggcctggc cctctgaggg tccccaggga
     1441 ggaaacgggc accacccgct ttcttgctgg taccgcggcc gcggatctcc ctttagtgag
     1501 ggttaattgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     1561 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     1621 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     1681 cagggggagg tgtgggaggt tttttctgca ttaatgaatc ggccaacgcg cggggagagg
     1741 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1801 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1861 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1921 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1981 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2041 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2101 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2161 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2221 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2281 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2341 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     2401 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2461 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2521 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2581 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2641 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2701 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2761 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2821 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2881 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2941 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3001 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3061 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3121 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3181 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3241 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3301 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3361 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3421 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3481 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3541 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3601 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3661 tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata ttttgttaaa
     3721 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3781 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3841 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3901 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3961 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4021 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4081 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4141 gggcgcgtcc cattcgccat tcacacagga aacagctatg accatgatcc tctagagtcg
     4201 acctgcaggc atgcaagcta ggcctacatg gcca
//
