LOCUS       40924_41245        5936 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSRalphapuroG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection 
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 5936)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5936)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard des 
            Prairies, Laval, Quebec H7V 1B7, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    100..686
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    786..1358
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1381..2097
                     /codon_start=1
                     /label=hrGFP
                     /note="humanized Renilla green fluorescent protein"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     polyA_signal    complement(2144..2278)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2352..2948)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTLDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(2958..3287)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        3322..3426
                     /label=AmpR promoter
     CDS             3427..4284
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4458..5046
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5308..5921
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
ORIGIN
        1 gaattcctgc agcccgtggc cactagtact tctcgagctc tgtacatgtc cgcggtcgcg
       61 acgtacgcgt cgagcatgca tctagggcgg ccaattccgc ccctctccct cccccccccc
      121 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
      181 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
      241 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
      301 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
      361 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
      421 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
      481 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
      541 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
      601 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      661 aacacgatga taagcttgcc acaacccctg caggcggccg ccatatgcat cctaggccta
      721 ttaatattcc ggagtatacg tagccggcta acgttaacaa ccggtatcta gggcggccaa
      781 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
      841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
      901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
      961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     1021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     1081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     1141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     1201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     1261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     1321 cacggggacg tggttttcct ttgaaaaaca cgatgataat ctagaactat agctagcacc
     1381 atggtgagca agcagatcct gaagaacacc ggcctgcagg agatcatgag cttcaaggtg
     1441 aacctggagg gcgtggtgaa caaccacgtg ttcaccatgg agggctgcgg caagggcaac
     1501 atcctgttcg gcaaccagct ggtgcagatc cgcgtgacca agggcgcccc cctgcccttc
     1561 gccttcgaca tcctgagccc cgccttccag tacggcaacc gcaccttcac caagtacccc
     1621 gaggacatca gcgacttctt catccagagc ttccccgccg gcttcgtgta cgagcgcacc
     1681 ctgcgctacg aggacggcgg cctggtggag atccgcagcg acatcaacct gatcgaggag
     1741 atgttcgtgt accgcgtgga gtacaagggc cgcaacttcc ccaacgacgg ccccgtgatg
     1801 aagaagacca tcaccggcct gcagcccagc ttcgaggtgg tgtacatgaa cgacggcgtg
     1861 ctggtgggcc aggtgatcct ggtgtaccgc ctgaacagcg gcaagttcta cagctgccac
     1921 atgcgcaccc tgatgaagag caagggcgtg gtgaaggact tccccgagta ccacttcatc
     1981 cagcaccgcc tggagaagac ctacgtggag gacggcggct tcgtggagca gcacgagacc
     2041 gccatcgccc agctgaccag cctgggcaag cccctgggca gcctgcacga gtgggtgtga
     2101 gagctctccg gactcagatc tgtcatgatg atcattgcaa ttggatccag acatgataag
     2161 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2221 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2281 caacaacaat tcgatgcatg gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg
     2341 ggcgggcgtc aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc
     2401 tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg
     2461 gaggtctcca ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg
     2521 acggcgctgc ccagaccctt gccctggtgg tcgggcgaga cgccgacggt ggccaggaac
     2581 cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc
     2641 agccgggaac cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct
     2701 tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc
     2761 ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg
     2821 atgtggcggt ccgggtcgag ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg
     2881 agggtgcgta cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac
     2941 tcggtcatgg taagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     3001 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     3061 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     3121 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     3181 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
     3241 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagaggt
     3301 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3361 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3421 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3481 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3661 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3961 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4261 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5041 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5101 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5161 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5221 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     5281 ataagcttga tagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5341 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5401 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5461 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5521 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5581 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5641 ctcctcgatc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5701 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5761 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5821 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5881 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctcct cgaatc
//
