LOCUS       40924_41235        6423 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSRalphaneoR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection 
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 6423)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6423)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard des 
            Prairies, Laval, Quebec H7V 1B7, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    100..686
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    786..1358
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1431..2105
                     /codon_start=1
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     polyA_signal    complement(2129..2263)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2297..3088)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(3445..3774)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        3809..3913
                     /label=AmpR promoter
     CDS             3914..4771
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4945..5533
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5795..6408
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
ORIGIN
        1 gaattcctgc agcccgtggc cactagtact tctcgagctc tgtacatgtc cgcggtcgcg
       61 acgtacgcgt cgagcatgca tctagggcgg ccaattccgc ccctctccct cccccccccc
      121 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
      181 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
      241 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
      301 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
      361 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
      421 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
      481 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
      541 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
      601 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      661 aacacgatga taagcttgcc acaacccctg caggcggccg ccatatgcat cctaggccta
      721 ttaatattcc ggagtatacg tagccggcta acgttaacaa ccggtatcta gggcggccaa
      781 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
      841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
      901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
      961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     1021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     1081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     1141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     1201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     1261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     1321 cacggggacg tggttttcct ttgaaaaaca cgatgataat ctagaactat agctagcgtt
     1381 taaacttaag cttggtaccg agctcggatc cccgggtacc ggtcgccacc atggcctcct
     1441 ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg catggagggc accgtgaacg
     1501 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc cacaacaccg
     1561 tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     1621 agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc gactacaaga
     1681 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     1741 tggcgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac aaggtgaagt
     1801 tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca gaagaagacc atgggctggg
     1861 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag acccacaagg
     1921 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc tacatggcca
     1981 agaagcccgt gcagctgccc ggctactact acgtggacgc caagctggac atcacctccc
     2041 acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc caccacctgt
     2101 tcctgtagcg gccgcgactc tagaattgga tccagacatg ataagataca ttgatgagtt
     2161 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2221 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaatcgaac
     2281 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     2341 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     2401 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     2461 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     2521 cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca
     2581 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     2641 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     2701 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     2761 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     2821 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     2881 gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct
     2941 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     3001 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     3061 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
     3121 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     3181 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     3241 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     3301 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     3361 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     3421 agtgcttgcg gcagcgtgaa gctttttgca aaagcctagg cctccaaaaa agcctcctca
     3481 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     3541 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     3601 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     3661 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     3721 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     3781 cagaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3841 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3901 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3961 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4021 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4081 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4141 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     4201 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4261 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4321 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4381 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4441 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4501 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4561 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4621 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4681 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4741 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4801 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4861 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4921 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4981 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5041 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5101 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5161 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5221 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5281 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5341 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5401 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5461 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5521 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5581 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5641 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5701 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5761 acaccgcata agcttgatag cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc
     5821 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5881 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5941 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6061 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6121 caaaaagctc ctcgatcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     6181 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     6241 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     6301 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     6361 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctcctcga
     6421 atc
//
