LOCUS       40924_41225        6588 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSRalphahygroY, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection 
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 6588)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6588)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard des 
            Prairies, Laval, Quebec H7V 1B7, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    100..686
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    786..1358
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1431..2147
                     /codon_start=1
                     /label=EYFP
                     /note="enhanced YFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    complement(2173..2307)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(2343..2391)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(2436..3470)
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRGDRE
                     MGEAN"
     promoter        complement(3610..3939)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        3974..4078
                     /label=AmpR promoter
     CDS             4079..4936
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5110..5698
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5960..6573
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
ORIGIN
        1 gaattcctgc agcccgtggc cactagtact tctcgagctc tgtacatgtc cgcggtcgcg
       61 acgtacgcgt cgagcatgca tctagggcgg ccaattccgc ccctctccct cccccccccc
      121 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
      181 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
      241 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
      301 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
      361 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
      421 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
      481 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
      541 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
      601 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      661 aacacgatga taagcttgcc acaacccctg caggcggccg ccatatgcat cctaggccta
      721 ttaatattcc ggagtatacg tagccggcta acgttaacaa ccggtatcta gggcggccaa
      781 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
      841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
      901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
      961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     1021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     1081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     1141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     1201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     1261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     1321 cacggggacg tggttttcct ttgaaaaaca cgatgataat ctagaactat agctagcgtt
     1381 taaacttaag cttggtaccg agctcggatc cccgggtacc ggtcgccacc atggtgagca
     1441 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1501 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1561 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1621 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     1681 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1741 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1801 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1861 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1921 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1981 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     2041 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2101 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     2161 actctagaat tggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     2221 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2281 accattataa gctgcaataa acaagttaac aacaacaatt tgggaccgaa ccccgcgttt
     2341 atgaacaaac gacccaacac ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc
     2401 gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccat ctcccgatcc
     2461 ccacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
     2521 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
     2581 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
     2641 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct
     2701 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
     2761 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
     2821 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
     2881 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
     2941 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
     3001 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
     3061 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc
     3121 tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
     3181 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc
     3241 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag
     3301 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg
     3361 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt
     3421 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc
     3481 ccccgatctg gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt ttacgcactg
     3541 cctctccctg acgcgggata aagtggtatt ctcaaacata tctcgcaagc ctgtcttgtg
     3601 tccaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     3661 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     3721 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     3781 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     3841 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     3901 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagag gtggcacttt
     3961 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4021 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4081 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4141 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4201 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4261 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4321 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4381 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4441 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4501 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4561 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4621 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4681 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4741 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4801 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4861 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4921 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4981 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5041 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5221 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     5281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5761 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5821 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5881 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcataagctt
     5941 gatagcttgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6001 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6061 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6121 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6181 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6241 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcctcga
     6301 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     6361 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     6421 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     6481 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     6541 tgtttcgttt tctgttctgc gccgttacag atccaagctc ctcgaatc
//
