LOCUS       40924_41220        4663 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSRalphahygro, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection 
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 4663)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard des 
            Prairies, Laval, Quebec H7V 1B7, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..252
                     /label=multiple cloning site
                     /note="multiple cloning site"
     polyA_signal    complement(248..382)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(418..466)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(511..1545)
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRGDRE
                     MGEAN"
     promoter        complement(1685..2014)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2049..2153
                     /label=AmpR promoter
     CDS             2154..3011
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3185..3773
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4035..4648
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
ORIGIN
        1 gaattcctgc agcccgtggc cactagtact tctcgagctc tgtacatgtc cgcggtcgcg
       61 acgtacgcgt atcgatggcg ccagctgcag gcggccgcca tatgcatcct aggcctatta
      121 atattccgga gtatacgtag ccggctaacg ttaacaaccg gtacctctag aactatagct
      181 agcatgcgca aatttaaagc gctgatatcg atcgcgcgca gatctgtcat gatgatcatt
      241 gcaattggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      301 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      361 tataagctgc aataaacaag ttaacaacaa caatttggga ccgaaccccg cgtttatgaa
      421 caaacgaccc aacacccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
      481 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc cccatctccc gatccccacg
      541 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
      601 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
      661 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
      721 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
      781 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
      841 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
      901 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
      961 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     1021 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     1081 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     1141 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     1201 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     1261 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc
     1321 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     1381 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     1441 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     1501 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccg
     1561 atctggcgca ctaaaaacca gtatttcaca tgagtccgcg tctttttacg cactgcctct
     1621 ccctgacgcg ggataaagtg gtattctcaa acatatctcg caagcctgtc ttgtgtccaa
     1681 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     1741 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     1801 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     1861 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     1921 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     1981 agcctgggga ctttccacac cctaactgac acacattcca cagaggtggc acttttcggg
     2041 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2101 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2161 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2221 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2281 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2341 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     2401 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2461 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2521 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2581 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2641 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     2701 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2761 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2821 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2881 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2941 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3001 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3061 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3301 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3841 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3901 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3961 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata agcttgatag
     4021 cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4081 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4141 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4201 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4261 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4321 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ctcgatcgag
     4381 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
     4441 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
     4501 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
     4561 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
     4621 cgttttctgt tctgcgccgt tacagatcca agctcctcga atc
//
