LOCUS       V003055                 5800 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003055
VERSION     V003055
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 5800)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5800)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard des
            Prairies, Laval, Quebec H7V 1B7, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    100..686
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     misc_feature    786..1358
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             1431..2147
                     /label="ECFP"
                     /note="enhanced CFP"
     polyA_signal    complement(2173..2307)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(2316..2735)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     promoter        complement(2755..2802)
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     promoter        complement(2822..3151)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        3186..3290
                     /label="AmpR promoter"
     CDS             3291..4148
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4322..4910
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        5172..5785
                     /label="SR-alpha promoter"
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human
                     T-lymphotrophic virus 1"
ORIGIN
        1 gaattcctgc agcccgtggc cactagtact tctcgagctc tgtacatgtc cgcggtcgcg
       61 acgtacgcgt cgagcatgca tctagggcgg ccaattccgc ccctctccct cccccccccc
      121 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
      181 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
      241 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
      301 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
      361 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
      421 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
      481 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
      541 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
      601 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      661 aacacgatga taagcttgcc acaacccctg caggcggccg ccatatgcat cctaggccta
      721 ttaatattcc ggagtatacg tagccggcta acgttaacaa ccggtatcta gggcggccaa
      781 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
      841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
      901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
      961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     1021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     1081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     1141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     1201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     1261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     1321 cacggggacg tggttttcct ttgaaaaaca cgatgataat ctagaactat agctagcgtt
     1381 taaacttaag cttggtaccg agctcggatc cccgggtacc ggtcgccacc atggtgagca
     1441 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1501 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1561 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1621 ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1681 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1741 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1801 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1861 acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     1921 ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1981 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2041 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2101 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     2161 actctagaat tggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     2221 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2281 accattataa gctgcaataa acaagttaac gtttagttcc tggtgtactt gagggggatg
     2341 agttcctcaa tggtggtttt gaccagcttg ccattcatct caatgagcac aaagcagtca
     2401 ggagcatagt cagagatgag ctctctgcac atgccacagg ggctgaccac cctgatggat
     2461 ctgtccacct catcagagta ggggtgcctg acagccacaa tggtgtcaaa gtccttctgc
     2521 ccgttgctca cagcagaccc aatggcaatg gcttcagcac agacagtgac cctgccaatg
     2581 taggcctcaa tgtggacagc agagatgatc tccccagtct tggtcctgat ggccgccccg
     2641 acatggtgct tgttgtcctc atagagcatg gtgatcttct cagtggcgac ctccaccagc
     2701 tccagatcct gctgagagat gttgaaggtc ttcatgatgg ccctcctata gtgagtcgta
     2761 ttatactatg ccgatatact atgccgatga ttaattgtca actactgttt aaattaagct
     2821 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     2881 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     2941 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     3001 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     3061 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     3121 ctggggactt tccacaccct aactgacaca cattccacag aggtggcact tttcggggaa
     3181 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3241 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3301 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3361 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3421 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3481 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     3541 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3601 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3661 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3721 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3781 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     3841 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3901 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3961 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4021 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4081 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4141 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4201 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4441 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4621 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4981 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5041 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     5101 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataagc ttgatagctt
     5161 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5221 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5281 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5341 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5401 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5461 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcctc gatcgagggg
     5521 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
     5581 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
     5641 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
     5701 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
     5761 tttctgttct gcgccgttac agatccaagc tcctcgaatc
//
