LOCUS       V003056                 3875 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003056
VERSION     V003056
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 3875)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3875)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard des
            Prairies, Laval, Quebec H7V 1B7, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..252
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     polyA_signal    complement(248..382)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(391..810)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     promoter        complement(830..877)
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     promoter        complement(897..1226)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        1261..1365
                     /label="AmpR promoter"
     CDS             1366..2223
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2397..2985
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3247..3860
                     /label="SR-alpha promoter"
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human
                     T-lymphotrophic virus 1"
ORIGIN
        1 gaattcctgc agcccgtggc cactagtact tctcgagctc tgtacatgtc cgcggtcgcg
       61 acgtacgcgt atcgatggcg ccagctgcag gcggccgcca tatgcatcct aggcctatta
      121 atattccgga gtatacgtag ccggctaacg ttaacaaccg gtacctctag aactatagct
      181 agcatgcgca aatttaaagc gctgatatcg atcgcgcgca gatctgtcat gatgatcatt
      241 gcaattggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      301 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      361 tataagctgc aataaacaag ttaacgttta gttcctggtg tacttgaggg ggatgagttc
      421 ctcaatggtg gttttgacca gcttgccatt catctcaatg agcacaaagc agtcaggagc
      481 atagtcagag atgagctctc tgcacatgcc acaggggctg accaccctga tggatctgtc
      541 cacctcatca gagtaggggt gcctgacagc cacaatggtg tcaaagtcct tctgcccgtt
      601 gctcacagca gacccaatgg caatggcttc agcacagaca gtgaccctgc caatgtaggc
      661 ctcaatgtgg acagcagaga tgatctcccc agtcttggtc ctgatggccg ccccgacatg
      721 gtgcttgttg tcctcataga gcatggtgat cttctcagtg gcgacctcca ccagctccag
      781 atcctgctga gagatgttga aggtcttcat gatggccctc ctatagtgag tcgtattata
      841 ctatgccgat atactatgcc gatgattaat tgtcaactac tgtttaaatt aagctttttg
      901 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
      961 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     1021 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     1081 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     1141 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     1201 gactttccac accctaactg acacacattc cacagaggtg gcacttttcg gggaaatgtg
     1261 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1321 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1381 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1441 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1501 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1561 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     1621 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1681 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1741 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1801 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1861 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     1921 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1981 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2041 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2101 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2161 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2221 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2281 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2341 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2401 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2461 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2521 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     2581 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2641 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2701 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2761 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2821 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2881 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2941 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3001 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3061 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3121 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     3181 tattttctcc ttacgcatct gtgcggtatt tcacaccgca taagcttgat agcttggctg
     3241 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3301 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3361 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3421 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3481 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3541 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgatcg aggggctcgc
     3601 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     3661 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     3721 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     3781 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     3841 gttctgcgcc gttacagatc caagctcctc gaatc
//
