LOCUS       V003046                 4755 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003046
VERSION     V003046
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Rodriguez MD, Paul Z, Wood CE, Rice KC, Triplett EW.
  TITLE     Construction of Stable Fluorescent Reporter Plasmids for Use in
            Staphylococcus aureus
  JOURNAL   Front Microbiol 8, 2491 (2017)
   PUBMED   29312199
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Rodriguez MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2017) Microbiology and Cell Science, University of
            Florida, 1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700,
            USA
REFERENCE   3  (bases 1 to 4755)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4755)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol 8, 2491 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-AUG-2017) Microbiology and Cell Science, University of Florida,
            1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label="repF"
                     /protein_id="AUS76875.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label="repF"
     rep_origin      1179..1567
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /label="sarA P1 promoter"
                     /note="sarA P1 promoter"
                     /regulatory_class="promoter"
     CDS             1847..2329
                     /gene="dfrA"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Tn4003 from
                     Staphylococcus aureus. Accession#: P13955"
     CDS             2367..3041
                     /label="DsRed-Express"
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     regulatory      3481..3838
                     /label="atl transcription terminator"
                     /note="atl transcription terminator"
                     /regulatory_class="terminator"
     rep_origin      3935..4112
                     /label="sso"
                     /note="sso"
     CDS             complement(4333..4344)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     misc_feature    4581..4601
                     /label="dso"
                     /note="dso"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 aataagacaa acgcaatata ttgtgtcaca aaaataagac agtacagctt tgtatgatcc
     1141 gtcgacgaaa gcctggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
     1201 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
     1261 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
     1321 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
     1381 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
     1441 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
     1501 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg
     1561 gctgacatgg gaattcgagc tgatattttt gactaaacca aatgctaacc cagaaataca
     1621 atcactgtgt ctaatgaata atttgtttta taaacacttt tttgtttact tctcattttt
     1681 aattagttat aattaactaa ataatagagc attaaatata tttaataaaa cttatttaat
     1741 gcaaaattat gactaacata tctataataa ataaagatta gatatcaata tattatcggg
     1801 caaatgtatc gagcaagatg catcggatcg atccaggagg tataccatga cattatcaat
     1861 aattgtcgct cacgataaac aaagagtcat tgggtaccaa aatcaattac cttggcactt
     1921 accaaatgat ttaaagcata ttaaacaact gaccactggg aatacacttg taatggcacg
     1981 gaaaactttt aattctatag ggaagccatt gccaaataga cgtaacgtcg tactcactaa
     2041 ccaagcttca tttcgccatg aaggggtaga tgttataaac tctcttgatg aaattaaaga
     2101 gttatctggt catgttttta tatttggagg acaaacgtta tacgaagcaa tgattgacca
     2161 ggtagatgat atgtatatca cagtaataga tggaaagttt caaggagaca cattctttcc
     2221 accatacaca ttcgaaaact gggaagtcga atcttcagta gaaggtcaac tagatgaaaa
     2281 aaatactata ccgcatacat tcttacattt agtgcgtaga aaagggaaat aggcgcgcct
     2341 attaacttta taaggaggaa aaacatatgg ataatacaga agatgttatt aaagaattta
     2401 tgcgttttaa agttcgtatg gaaggttcag ttaatggtca tgaatttgaa attgaaggtg
     2461 aaggtgaagg acgtccatat gaaggtacac aaacagcaaa attaaaagtt acaaaaggtg
     2521 gtccattacc atttgcatgg gatattttat caccacaatt tcaatatggt tcaaaagttt
     2581 atgttaaaca tccagcagat attccagatt ataagaaatt atcatttcct gaaggtttta
     2641 aatgggaacg tgttatgaat tttgaagatg gtggtgttgt tacagttaca caagattcat
     2701 cattacaaga tggttgtttt atctataaag ttaaatttat tggtgttaat tttccatcag
     2761 atggtccagt tatgcaaaag aaaacaatgg gttgggaacc atcaacagaa cgtttatatc
     2821 cacgtgatgg tgttttaaaa ggtgaaattc ataaagcatt gaaattaaaa gatggtggtc
     2881 attatttagt tgaatttaaa tcaatttata tggcaaaaaa accagttcaa ttaccaggtt
     2941 attattatgt tgattcaaaa ttagatatta catcacataa tgaagattat acaattgttg
     3001 aacaatatga acgtacagaa ggtcgtcatc atttgttttt ataataggcg cgcctattct
     3061 aatgcataat aaatactgat aacatcttat attttgtatt atattttgta ttatcgttga
     3121 catgtataat tttgatatca aaaactgatt ttccctctat tattttcgag atttattttc
     3181 ttaattctct ttaacaaact agaaatattg tatatacaaa aaattataaa taatagatga
     3241 atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt aaagtgcgtt
     3301 gcttttttct catttataag gttaaataat tctcatatat caagcaaagt gacaggcgat
     3361 gcggccgcta gcctaggagc tcggtacccg gggatccgaa tcatgaatta caagcaaaag
     3421 tagcggtgat tgttaaaatt gatggtaaac aatcaccgct atttttgctt gtgtatgtat
     3481 aaaaaaggga tcaaaggtca tcccccatga ttgatagtgg ggggatgact tttgatccta
     3541 tgttcatgtt gcttatttaa tcgcctttga tcactttaaa ataccttaaa acccctggaa
     3601 tttctggctt tgccagacct atcatttttg aatgatagca aattctcctt atgctcttac
     3661 ggagttttta gagaaaaatt aaaaattctc gatttttgat aaaaaacgcc ctgcaggaat
     3721 ttagaaaaac atgtggaagt tttaaaggat tttatgctaa tttttaattt gcatgtaact
     3781 cgaggggaat atttgagggg attttgaaac gagtttcttc ttgttttcac actgtttttt
     3841 tattcctatt ggtgttgttg cttacttttt gtttttctat aaagatgatg cttttgacga
     3901 gatggaagaa aaatatgatt atcatgaaga taataaaaaa tagacgacgc atttatgccg
     3961 agaaaattta ttgatgttga gaagaaccct taactaaact tgcagacgaa tgtcggcata
     4021 gcgtgagcta ttaagccgac cattcgacaa gttttgggat tgttaagggt tccgaggctc
     4081 aacgtcaata aagcaattgg aataaatagt atctaaagta acccgtattt aagattacat
     4141 tgtacatcct tcgaaaccat agtagctgcg attccagcta ggtttccttt tatcccaatt
     4201 ttagctaatt tttttgctac gccctttaag tttttggctt taacatcttc aaaaactgct
     4261 gtgattatgt ttccagttaa aagttctcca tatgagttta aaacttcaga atagaaacag
     4321 tcaccgctat ttttgtagtt cccaccaact gctctaggtg atattgaact agttttcttt
     4381 ttattctcta tgtcgttttt tagtttttgg aatccttcag gaacatatcc atatcgagat
     4441 ttggccgatt ttatttgata atccttttga ctttttagaa agttttattt attcattggt
     4501 tttcacatta atttatttag gtttagaggg tcataaaaag aagaaggaat aggttgtttt
     4561 ttgaaacgag tgtgaacgag tttcttcttg tcttgatact atatagaaat aactcgattt
     4621 tatatatata gctgtaactg ttgatattac agtgtttaaa cgtgtttttg tgcgtgaaag
     4681 gaaaatttga caataaaaaa ccccagttat attattaagg tgtcgaatct taaataatac
     4741 tgggggtctt tttat
//
