LOCUS       40924_41255        3732 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pSRF-Luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-DEC-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 3732)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-1999) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   4  (bases 1 to 3732)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3732)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-MAR-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-DEC-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (01-FEB-1999) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Feb 1, 1999 this sequence version replaced gi:4071313.
FEATURES             Location/Qualifiers
     source          1..3732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1547..1565)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1586..1602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1610..1626)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1634..1664)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1679..1700)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1988..2576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2750..3607)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3608..3712)
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gacggccgct taactctaga ataatggaat acccttttga actggcatga
      721 ttaagactcc ttattacgca gtatgttagc aaacgtagaa aatacataca taaaggtggc
      781 aacatataaa agaaacgcaa agacaccacg gaataagttt attttgtcac aatcaataga
      841 aaattcatat ggtttaccag cgccaaagac aaaagggcga cattcaaccg attgagggag
      901 ggaaggtaaa tattgacgga aattattcat taaaggtgaa ttatcaccgt caccgacttg
      961 agccatttgg gaattagagc cagcaaaatc accagtagca ccattaccat tagcaaggcc
     1021 ggaaacgtca ccaatgaaac catcgatagc agcaccgtaa tcagtagcga cagaatcaag
     1081 tttgccttta gcgtcagact gtagcgcgtt ttcatcggca ttttcggtca tagccccctt
     1141 attagcgttt gccatctttt cataatcaaa atcaccggaa ccagagccac caccggaacc
     1201 gcctccctca gagccgccac cctcagaacc gccaccctca gagccaccac cctcagagcc
     1261 gccaccagaa ccaccaccag agccgccgcc agcattgaca ggaggttgag gcaggtcaga
     1321 cgattggccc tgatattcac aaacgaatgg gctacctcca cctccactag taaggacggc
     1381 ttctcattag gccggttggg ccgcgagtaa taacaatcca gcggctgccg taggcaatag
     1441 gtatttcatt atgactgtct ccttggcggc cgccgtaggc aataggtatt tcattatgac
     1501 tgtctccttg gcgagtagtt aagaattcaa gctccagctt ttgttccctt tagtgagggt
     1561 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1621 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2161 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2401 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3301 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3361 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3421 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3481 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3541 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3601 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3661 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3721 cccgaaaagt gc
//
