LOCUS       40924_41250        5743 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pSRE-Luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Zheng C-F.
  TITLE     pSRE-Luc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines, San Diego, CA 92037, USA
REFERENCE   3  (bases 1 to 5743)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines, San Diego, CA 92037, USA
REFERENCE   4  (bases 1 to 5743)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-1999) Technical Services, Stratagene, 11011 N. 
            Torrey Pines, San Diego, CA 92037, USA
REFERENCE   5  (bases 1 to 5743)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 5743)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-1998) Technical Services, Stratagene, 11011 N. Torrey Pines,
            San Diego, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines,
            San Diego, CA 92037, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (01-FEB-1999) Technical Services, Stratagene, 11011 N. Torrey Pines,
            San Diego, CA 92037, USA"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     On Feb 1, 1999 this sequence version replaced gi:3165517.
FEATURES             Location/Qualifiers
     source          1..5743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..136
                     /label=AmpR promoter
     CDS             137..994
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1168..1755
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1934..2074)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     polyA_signal    2313..2445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2539..2671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2805..2836
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2863..4512
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          4646..4711
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5281..5415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttcact gcattctagt
     2401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca tatcacattt
     2461 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2521 atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2581 agcatcacaa atttcacaaa taaagcaatt tttcactgca ttctattgtg gtttgtccaa
     2641 actcatcaat gtatcttatc atgtctggat ccaagctagg atgtccatat taggacatct
     2701 aggatgtcca tattaggaca tctaggatgt ccatattagg acatctagga tgtcccatat
     2761 tagggacatc taggatgtcc atattaggac atctgcggag actctagagg gtatataatg
     2821 gaagctcgaa ttccagcttg gcattccggt actgttggta aaatggaaga cgccaaaaac
     2881 ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg agagcaactg
     2941 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
     3001 atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct
     3061 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
     3121 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
     3181 gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc taccgtagtg
     3241 tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc
     3301 cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     3361 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc agagtccttt
     3421 gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg gttacctaag
     3481 ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag agatcctatt
     3541 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     3601 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     3661 agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca aagtgcgttg
     3721 ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa atacgattta
     3781 tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt cggggaagcg
     3841 gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac tgagactaca
     3901 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     3961 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcag
     4021 agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa caatccggaa
     4081 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     4141 gacgaagacg aacacttctt catagttgac cgcttgaagt ctttaattaa atacaaagga
     4201 tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa catcttcgac
     4261 gcgggcgtgg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     4321 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     4381 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     4441 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     4501 aagtccaaat tgtaaaatgt aactgtattc agcgatgacg aaattcttag ctattgtaat
     4561 actctagagg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     4621 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     4681 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactt atgaatggga
     4741 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     4801 tgatgatgag gctactgctg actctcaaca ttctactctc aaaagaagag aaaggtagag
     4861 acccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     4921 aactcttgct tgctttgcta tttacaacca caaaggaaaa agctgcactg ctatacaaga
     4981 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     5041 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     5101 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     5161 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     5221 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5401 tatcatgtct ggatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac
     5461 ttgagaggac attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag
     5521 gtcacttaac aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taaggggtaa
     5581 ttttaaaata tctgggaagt cccttccact gctgtgttcc agaagtgttg gtaaacagcc
     5641 cacaaatgtc aacagcagaa acatacaagc tgtcactttg cacaaagggc ctcgtgatac
     5701 gcctattttt ataggttaat gtcatgataa taatggtttc tta
//
