LOCUS       V016230                 6282 bp    DNA     circular SYN 27-JUL-2020
DEFINITION  Exported.
ACCESSION   V016230
VERSION     V016230
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Optogenetic control of gut bacterial metabolism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2019) Bioengineering, Rice University, 6500 Main
            St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 6282)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-OCT-2019) Bioengineering, Rice University, 6500 Main St.,
            Houston, TX 77030, USA"
FEATURES             Location/Qualifiers
     source          1..6282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        111..145
                     /label="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     regulatory      162..173
                     /label="sRBS"
                     /note="sRBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             180..899
                     /gene="pbsA1"
                     /label="Heme oxygenase 1"
                     /note="Heme oxygenase 1 from Synechocystis sp. (strain PCC
                     6803 / Kazusa). Accession#: P72849"
     regulatory      905..916
                     /label="RBS pprotet"
                     /note="RBS pprotet"
                     /regulatory_class="ribosome_binding_site"
     CDS             923..1669
                     /codon_start=1
                     /transl_table=11
                     /product="PcyA"
                     /label="PcyA"
                     /protein_id="QLL27775.1"
                     /translation="MAVTDLSLTNSSLMPTLNPMIQQLALAIAASWQSLPLKPYQLPED
                     LGYVEGRLEGEKLVIENRCYQTPQFRKMHLELAKVGKGLDILHCVMFPEPLYGLPLFGC
                     DIVAGPGGVSAAIADLSPTQSDRQLPAAYQKSLAELGQPEFEQQRELPPWGEIFSEYCL
                     FIRPSNVTEEERFVQRVVDFLQIHCHQSIVAEPLSEAQTLEHRQGQIHYCQQQQKNDKT
                     RRVLEKAFGEAWAERYMSQVLFDVIQ"
     terminator      1684..1755
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1771..1798
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     regulatory      1850..1997
                     /note="specR promoter; from KD13"
                     /regulatory_class="promoter"
     CDS             1998..2786
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     terminator      2801..2895
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      3009..3554
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3810..3844
                     /label="constitutive promoter J23106"
                     /note="constitutive promoter J23106"
                     /regulatory_class="promoter"
     misc_feature    3845..3879
                     /label="B0034"
                     /note="B0034"
     CDS             3880..6141
                     /codon_start=1
                     /transl_table=11
                     /product="CcaS"
                     /label="CcaS"
                     /protein_id="QLL27774.1"
                     /translation="MGKFLIPIEFVFLAIAMTCYLWHRQNQERRRIEISIKQQTQRERF
                     INQITQHIRQSLNLETVLNTTVAEVKTLLQVDRVLIYRIWQDGTGSAITESVNANYPSI
                     LGRTFSDEVFPVEYHQAYTKGKVRAINDIDQDDIEICLADFVKQFGVKSKLVVPILQHN
                     RASSLDNESEFPYLWGLLITHQCAFTRPWQPWEVELMKQLANQVAIAIQQSELYEQLQQ
                     LNKDLENRVEKRTQQLAATNQSLRMEISERQKTEAALRHTNHTLQSLIAASPRGIFTLN
                     LADQIQIWNPTAERIFGWTETEIIAHPELLTSNILLEDYQQFKQKVLSGMVSPSLELKC
                     QKKDGSWIEIVLSAAPLLDSEENIAGLVAVVADITEQKRQAEQIRLLQSVVVNTNDAVV
                     ITEAEPIDDPGPRILYVNEAFTKITGYTAEEMLGKTPRVLQGPKTSRTELDRVRQAISQ
                     WQSVTVEVINYRKDGSEFWVEFSLVPVANKTGFYTHWIAVQRDVTERRRTEEVRLALER
                     EKELSRLKTRFFSMASHEFRTPLSTALAAAQLLENSEVAWLDPDKRSRNLHRIQNSVKN
                     MVQLLDDILIINRAEAGKLEFNPNWLDLKLLFQQFIEEIQLSVSDQYYFDFICSAQDTK
                     ALVDERLVRSILSNLLSNAIKYSPGGGQIKIALSLDSEQIIFEVTDQGIGISPEDQKQI
                     FEPFHRGKNVRNITGTGLGLMVAKKCVDLHSGSILLKSAVDQGTTVTICLKRYNHLPRA
                     "
     terminator      complement(6165..6208)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gtaaatcact gcataattcg tgtcgattaa accgcctaag tcccccagga aagggggata
       61 taacagtata gattttgtca gccttcagct tggctttacc gtcaaaaaaa ctgacagcta
      121 gctcagtcct aggtataatg ctagctcaca cagaattcat taaagaggag aaaggtacca
      181 tgagtgtcaa cttagcttcc cagttgcggg aagggacgaa aaaatcccac tccatggcgg
      241 agaacgtcgg ctttgtcaaa tgcttcctca agggcgttgt cgagaaaaat tcctaccgta
      301 agctggttgg caatctctac tttgtctaca gtgccatgga agaggaaatg gcaaaattta
      361 aggaccatcc catcctcagc cacatttact tccccgaact caaccgcaaa caaagcctag
      421 agcaagacct gcaattctat tacggctcca actggcggca agaagtgaaa atttctgccg
      481 ctggccaagc ctatgtggac cgagtccggc aagtggccgc tacggcccct gaattgttgg
      541 tggcccattc ctacacccgt tacctggggg atctttccgg cggtcaaatt ctcaagaaaa
      601 ttgcccaaaa tgccatgaat ctccacgatg gtggcacagc tttctatgaa tttgccgaca
      661 ttgatgacga aaaggctttt aaaaatacct accgtcaagc tatgaatgat ctgcccattg
      721 accaagccac cgccgaacgg attgtggatg aagccaatga cgcctttgcc atgaacatga
      781 aaatgttcaa cgaacttgaa ggcaacctga tcaaggcgat cggcattatg gtgttcaaca
      841 gcctcacccg tcgccgcagt caaggcagca ccgaagttgg cctcgccacc tccgaaggct
      901 agttaaagag gagaaaggat ccatggccgt cactgattta agtttgacca attcttccct
      961 gatgcctacg ttgaacccga tgattcaaca gttggccctg gcgatcgccg ctagttggca
     1021 aagtttaccc ctcaagccct atcaattgcc ggaggatttg ggctacgtag aaggccgcct
     1081 ggaaggggaa aagttagtga ttgaaaatcg gtgctaccaa acgccccagt ttcgcaaaat
     1141 gcatttggag ttggccaagg tgggcaaagg gttggatatt ctccactgtg taatgtttcc
     1201 tgagccttta tacggtctac ctttgtttgg ctgtgacatt gtggccggcc ccggtggagt
     1261 aagtgcggct attgcggatc tatcccccac ccaaagcgat cgccaattgc ccgcagcgta
     1321 ccaaaaatca ttggcagagc taggccagcc agaatttgag caacaacggg aattgccccc
     1381 ctggggagaa atattttctg aatattgttt attcatccgt cccagcaatg tcactgaaga
     1441 agaaagattt gtacaaaggg tagtggactt tttgcaaatt cattgtcacc aatccatcgt
     1501 tgccgaaccc ttgtctgaag ctcaaacttt ggagcaccgt caggggcaaa ttcattactg
     1561 ccaacaacaa cagaaaaatg ataaaacccg tcgggtactg gaaaaagctt ttggggaagc
     1621 ttgggcggaa cggtatatga gccaagtctt atttgatgtt atccaataat ctagaccagg
     1681 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1741 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1801 tataggtacc cggggatcct ctagagtcga cctgcaggca tgcaagctta gcaagcgaac
     1861 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
     1921 tggctttctt gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag
     1981 gatgaggatc gtttcgcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2041 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2101 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2161 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2221 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2281 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2341 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2401 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2461 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2521 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2581 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2641 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2701 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     2761 gcgagatcac caaggtagtc ggcaaataag agctcgcttg gactcctgtt gatagatcca
     2821 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
     2881 tttattggtg agaatccaag cactagtaac aacttatatc gtatggggct gacttcaggt
     2941 gctacatttg aagagataaa ttgcactgaa atctagtaat attttatctg attaataaga
     3001 tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc
     3061 gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac
     3121 tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat
     3181 gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc
     3241 atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg
     3301 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
     3361 caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     3421 caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat ctttatagtc
     3481 ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
     3541 ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct
     3601 ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac
     3661 gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcccctagg tggagtctgg
     3721 cctcaaatac aaagtctgca aagtctcgaa atattaaacc agagaataaa taattaaacg
     3781 ccaaaatatt tctagcaaaa accccagttt ttacggctag ctcagtccta ggtatagtgc
     3841 tagctctacc gctccagcaa actttaaggg aggttagcaa tgggcaaatt tctaattcca
     3901 atcgaatttg tttttctggc gatcgccatg acctgttatt tatggcacag acaaaaccaa
     3961 gaacgccgca ggattgaaat tagcatcaag caacaaaccc aacgggaacg atttattaac
     4021 caaattaccc aacatatccg ccaatcttta aacttggaaa cggttttaaa taccaccgtc
     4081 gctgaagtta aaaccctgtt gcaagttgat cgagttctaa tttatcgcat ttggcaagat
     4141 ggcacgggca gcgccattac ggaatcggtg aatgccaatt atcctagtat tttagggcgg
     4201 accttttccg atgaagtttt tcccgttgaa taccatcaag cctacaccaa aggtaaagta
     4261 cgggccatta atgacattga ccaggatgac atagagattt gcctagctga tttcgtcaaa
     4321 caatttggcg tgaaatcaaa attagtagtg cccattcttc aacataatcg tgcttcttcc
     4381 ctagataatg aatcagaatt tccctatctt tgggggctgt taattaccca tcaatgtgct
     4441 tttacccggc catggcaacc gtgggaagtg gagttaatga aacagctagc caatcaggtc
     4501 gcgatcgcca tccaacaatc ggaattatat gagcaattac agcaactcaa taaagatttg
     4561 gaaaaccgag tcgaaaaacg cacccagcaa cttgccgcca ccaatcaatc cctaagaatg
     4621 gaaatcagtg agcgacaaaa aacggaagcc gctctccgcc acactaacca tactctgcaa
     4681 tccctgattg cggcctcccc caggggtatt tttaccctta atttagcaga ccaaattcag
     4741 atttggaatc ctacagcaga acgtattttt ggttggacag aaacagaaat tattgcccat
     4801 ccagaattat taacatccaa cattttgctg gaagattatc agcaatttaa acagaaagtt
     4861 ttatcaggca tggtttcccc tagcctagaa ttaaaatgtc aaaaaaaaga tggtagttgg
     4921 attgaaattg tcctttccgc tgctccccta ttggatagtg aagaaaatat tgccggattg
     4981 gtggcggttg tcgccgatat taccgagcaa aagcggcagg cagaacaaat tcgtttgcta
     5041 caatccgttg tggttaatac taatgatgcg gtggtgatta cggaagcgga gcccattgat
     5101 gatcccgggc cgagaattct ctatgtcaat gaagcattta ctaaaatcac cggttatact
     5161 gctgaagaaa tgctaggcaa aaccccccga gttttacagg gaccaaaaac tagtcgcact
     5221 gaattagata gggtgcggca agccattagt caatggcaat cagttaccgt tgaagtgatt
     5281 aattatcgta aggatggcag tgagttttgg gtggaattta gtctggtgcc cgttgccaat
     5341 aaaacaggtt tttacaccca ttggattgct gtgcaaaggg atgtcactga gcgccgacgc
     5401 acggaggaag tccgcctagc tttagaacgg gaaaaagaat taagccgcct aaaaactcgt
     5461 tttttctcca tggcttccca tgaatttcgt actcccctca gtacggcctt agctgctgcc
     5521 caattactgg aaaattctga agtggcctgg cttgatcccg ataagcgtag ccggaactta
     5581 caccgtattc aaaattccgt gaaaaatatg gtacagctcc tggatgatat tttaatcatt
     5641 aaccgtgccg aagcgggcaa attggaattt aatcctaatt ggttagattt gaaattattg
     5701 ttccagcaat ttatcgaaga aattcaatta agtgtcagtg accaatatta ttttgacttt
     5761 atttgtagcg ctcaagatac gaaggcattg gtggatgaaa ggttagtgcg gtctatttta
     5821 tctaatctgt tatctaatgc gattaaatac tctcccgggg gagggcagat taaaattgcc
     5881 ctaagcctag attcggaaca gattattttt gaagtcaccg accagggcat tggcatttcg
     5941 ccagaggacc aaaagcaaat ttttgaaccc tttcatcggg gcaaaaatgt cagaaatatt
     6001 acgggaacag gactcggttt aatggttgcc aagaaatgtg ttgacttaca cagtggcagt
     6061 atcttgctaa aaagtgcagt tgaccaggga acaacagtta ctatctgttt aaaacgctat
     6121 aaccatttgc ctcgagctta gtaataatct agaccaggca tcaaataaaa cgaaaggctc
     6181 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     6241 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//
