LOCUS       62056_20160       13447 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13447)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     rep_origin      complement(2824..3448)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5932..6396
                     /codon_start=1
                     /label=VN155(I152L)
                     /note="improved N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     misc_feature    complement(7311..7335)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(8934..10079)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10381..11172)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11364..12131
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(12832..13442)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 gttaaccggg ctcaggcctg gcgcgccact agtggatcca tcgatagtac tgtcgacctc
     5881 gagggtaccg ctcccgggat ggagcaaaag ttgatttctg aggaggatct tatggtgagc
     5941 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     6001 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     6061 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     6121 accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     6181 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     6241 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     6301 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     6361 tacaactaca acagccacaa cgtctatatc atggcctaag agctcgaatt tccccgatcg
     6421 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     6481 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     6541 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     6601 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     6661 actagatcgg gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6721 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6781 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc
     6841 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6901 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6961 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7021 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7081 atctcgggct attcttttga tttataaggg attttgccga tttcggaacc accatcaaac
     7141 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     7201 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccccag
     7261 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     7321 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     7381 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     7441 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     7501 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     7561 ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca
     7621 tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat
     7681 cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac
     7741 gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc cgaagttccg
     7801 actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga gaaagcccag
     7861 taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa
     7921 acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg
     7981 gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg
     8041 ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc
     8101 gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc
     8161 gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc
     8221 catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg
     8281 cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa
     8341 gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc
     8401 cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag
     8461 cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc
     8521 cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc
     8581 gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg
     8641 ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga
     8701 caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt
     8761 aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt
     8821 cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc
     8881 agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg
     8941 caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct
     9001 tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac
     9061 atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg
     9121 aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag
     9181 tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt
     9241 ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca
     9301 acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc
     9361 ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg
     9421 aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc
     9481 agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg
     9541 acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg
     9601 aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca
     9661 atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg
     9721 gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg
     9781 gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt
     9841 tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt
     9901 tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg
     9961 aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg
    10021 gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt
    10081 caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt
    10141 gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg
    10201 tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc
    10261 agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg
    10321 cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta
    10381 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
    10441 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
    10501 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
    10561 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
    10621 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
    10681 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
    10741 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
    10801 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
    10861 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
    10921 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
    10981 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
    11041 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
    11101 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
    11161 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
    11221 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    11281 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    11341 ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag tgtatgatgg
    11401 tgtttttgag gtgctccagt ggcttctgtg tctatcagct gtccctcctg ttcagctact
    11461 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac
    11521 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag
    11581 aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg cattatgggc
    11641 gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc
    11701 gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggctatg
    11761 tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc cggaagacgg
    11821 ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc
    11881 tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc
    11941 tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc
    12001 tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg
    12061 tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg
    12121 gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa
    12181 gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc
    12241 tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat
    12301 attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc
    12361 tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa
    12421 ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc gctgggttta
    12481 tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa
    12541 aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc
    12601 cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca
    12661 cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg
    12721 ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg
    12781 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt
    12841 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
    12901 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
    12961 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
    13021 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
    13081 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
    13141 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
    13201 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
    13261 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    13321 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac
    13381 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
    13441 agcccgg
//
