LOCUS       40924_41190        3457 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Poxvirus recombinant transfer vector pSPV-EGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Luo S, Peng Y, Diel DG, Rock DL.
  TITLE     Virus recombinant transfer vector pSPV-EGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Luo S, Peng Y, Diel DG, Rock DL.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2009) Pathobiology, University of Illinois at 
            Urbana-Champaign, 2001 S. Lincoln Avenue, Urbana, IL 61802, USA
REFERENCE   3  (bases 1 to 3457)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3457)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2009) Pathobiology, University of Illinois at 
            Urbana-Champaign, 2001 S. Lincoln Avenue, Urbana, IL 61802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..33
                     /label=multiple cloning site 1 (MCS)
                     /note="multiple cloning site 1 (MCS)"
     regulatory      52..320
                     /label=early-late Vaccinia virus (VACV) VV7.5 promoter
                     /note="early-late Vaccinia virus (VACV) VV7.5 promoter"
                     /regulatory_class="promoter"
     CDS             340..1056
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    1060..1085
                     /label=multiple cloning site 2 (MCS2)
                     /note="multiple cloning site 2 (MCS2)"
     promoter        complement(1095..1113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1371..1959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2133..2990)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2991..3095)
                     /label=AmpR promoter
     promoter        3441..3457
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaactcgaga ctagtaggcc tgcgcgcaag cttgcatgcc tgcaggtcga cctgcagata
       61 tactatatag taataccaat actcaagact acgaaactga tacaatctct tatcatgtgg
      121 gtaatgttct cgatgtcgaa tagccatatg ccggtagttg cgatatacat aaactgatca
      181 ctaattccaa acccacccgc tttttatagt aagtttttca cccataaata ataaatacaa
      241 taattaattt ctcgtaaaag tagaaaatat attctaattt attgcacggt aaggaagtag
      301 aatcataaag aacagtgacg cctcgaggaa ttcgccacca tggtgagcaa gggcgaggag
      361 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      421 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      481 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      541 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      601 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      661 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      721 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      781 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      841 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      901 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
      961 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1021 gccgggatca ctctcggcat ggacgagctg tacaagtaag cggccgcatg catccgcgga
     1081 gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt taacctgcat
     1141 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1801 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2041 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2101 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2161 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2221 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2281 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2341 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2401 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2461 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2521 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2581 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2641 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2701 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2761 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2821 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2881 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2941 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3001 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3061 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3121 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3181 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     3241 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     3301 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     3361 ctgagagtgc accatatgga catattgtcg ttagaacgcg gctacaatta atacataacc
     3421 ttatgtatca tacacatacg atttaggtga cactata
//
