LOCUS       62056_20150        3104 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(7..63)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(71..89)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    248..390
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(576..1164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1338..2195)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2196..2300)
                     /label=AmpR promoter
     promoter        3088..3104
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatacaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
       61 ttccggtctc cctatagtga gtcgtattaa tttcgataag ccagctgggc ctcgcgcgtt
      121 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      181 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      241 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatata ctggcttaac
      301 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
      361 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
      421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
      781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     1021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1321 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1441 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1621 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1801 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2161 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2281 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2341 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     2401 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2461 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2521 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     2581 atcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     2641 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     2701 cggccacggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     2761 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     2821 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct
     2881 gattggttcg ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg
     2941 tccaaccaaa ccgactctga cggcagttta cgagagagat gatagggtct gcttcagtaa
     3001 gccagatgct acacaattag gcttgtacat attgtcgtta gaacgcggct acaattaata
     3061 cataacctta tgtatcatac acatacgatt taggtgacac tata
//
