LOCUS       40924_41032        2464 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector for in vitro transcription using the SP6 and T7 RNA 
            polymerase promoters. Essentially identical to pSP72 except for the 
            orientation of the MCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Krieg PA, Melton DA.
  TITLE     In vitro RNA synthesis with SP6 RNA polymerase.
  JOURNAL   Meth. Enzymol. 1987;155:397-415.
  PUBMED    2828872
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2464)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Meth. 
            Enzymol."; date: "1987"; volume: "155"; pages: "397-415"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    24..80
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(102..120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(378..966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1140..1997)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1998..2102)
                     /label=AmpR promoter
     promoter        2448..2464
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaaccagatc tgatatcatc gatgaattcg agctcggtac ccggggatcc tctagagtcg
       61 acctgcaggc atgcaagctt cagctgctcg aggccggtct ccctatagtg agtcgtatta
      121 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      181 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      541 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      781 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2101 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2161 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     2221 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     2281 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     2341 aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
     2401 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     2461 tata
//
