LOCUS       62056_20130        2880 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(518..536)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(794..1382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1556..2413)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2414..2518)
                     /label=AmpR promoter
     promoter        2864..2880
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaaccagatc tgatatcatc gatgaattcg ttctgtggat ctagcacctc cgccaaagct
       61 tctgccttct gcagcagaac ttcttctgct cggtctgcag ttgtcttctt gaggccattc
      121 agacttagac ctgggaactc tgggaacaga gcctggtcag gccttctgat gcccttcagt
      181 ggagccacgc ccatctggac ccccagtgtg ggaggcagtg ccaacagcag aacacaactc
      241 agcaacattg cagtcagcat ggcctttgct tgctgatctt tgcctaggga tgtcctggtc
      301 tcttgtgccc tgagcttata tggcagtgcc taataaaggg tccacacgtg actgcttcct
      361 gtagccaggg catgaggtgc ctccctaagg ttcctggaaa ccaagggagc agaaaacact
      421 gttcccccgg tgggcataca gcttcagagg acaaagcttt ggatcctcta gagtcgacct
      481 gcaggcatgc aagcttcagc tgctcgaggc cggtctccct atagtgagtc gtattaattt
      541 cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      601 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      661 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      721 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      781 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      841 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      901 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      961 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     1021 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1081 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1141 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1201 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     1261 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1321 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1381 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1441 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1501 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1561 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1621 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1681 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1741 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1801 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1861 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1921 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1981 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2041 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2101 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2161 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2221 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2281 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2341 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2401 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2461 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2521 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2581 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2641 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2701 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2761 atgcggcatc agagcagatt gtactgagag tgcaccatat ggacatattg tcgttagaac
     2821 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
//
