LOCUS       40924_41022        3628 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Microarray spiking control vector pSP64bb2sp5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Sato M, Mitra RM, Coller J, Wang D, Spivey NW, Dewdney J, Denoux C, 
            Glazebrook J, Katagiri F.
  TITLE     A high-performance, small-scale microarray for expression profiling 
            of many samples in Arabidopsis-pathogen studies
  JOURNAL   Plant J. 49 (3), 565-577 (2007)
  PUBMED    17181774
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Sato M, Katagiri F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2006) Department of Plant Biology, University of 
            Minnesota, 1500 Gortner Avenue, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 3628)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3628)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2007"; volume: "49"; issue: "3"; pages: "565-577"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2006) Department of Plant Biology, University of Minnesota, 
            1500 Gortner Avenue, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..684
                     /label=transcribed region
                     /note="transcribed region"
     misc_feature    1..579
                     /label=backbone sequence 2 (bb2)
                     /note="backbone sequence 2 (bb2)"
     misc_feature    580..643
                     /label=spiking control 5 (sp5)
                     /note="spiking control 5 (sp5)"
     primer_bind     complement(698..714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(722..738)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(746..776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(791..812)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1100..1688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1862..2719)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2720..2824)
                     /label=AmpR promoter
     promoter        3612..3628
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatacaagc ttattggcat cgcggaacag tgttttcaga attgcgtgga taacactcat
       61 gtgaccgtat aaactcagtt gggctgcaag agggcaatgt tattacccag actctatctc
      121 cacatgacgc acgtgtctac gtcgtcacga ctaaagctaa tggtgcgaaa gctaactccc
      181 ttagtagacg caaatgctga cgtacaggtc tgcgaagtcg cgtggtatct tgtaccttgt
      241 acgcgtcgag tgaaggatag aatatctcaa cagttaagct ataggagatc gacggctctc
      301 aacgttccga tcaatagcat atacttgacc tcacaattgc tcccagtagg tcaaatcttg
      361 tgccgattgc accgttaggg acctacatct atagactggg ggacacagtc gatttaatgc
      421 tatgaacaat cgttcgagcc agaaccaggg aaccggctta gcatggttcc acaaggttat
      481 ttataggtcg cgcggagttt taaggttgca ggacttaagt aggtagacct aaatgcccta
      541 ccggctaccg attgatgttt accgactctt ttacccggga ttcgagagct tttgtcgaca
      601 cgtttaccgc cgaacattcc atttaggtac cgggatggtg gcagagctcc caaaaaaaaa
      661 aaaaaaaaaa aaaaaaaaaa accgaattcg taatcatgtc atagctgttt cctgtgtgaa
      721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      841 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      901 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      961 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1021 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1081 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1141 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1201 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1261 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1321 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1381 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1441 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1501 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1561 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1621 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1681 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1741 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1801 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1861 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1921 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1981 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2041 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2101 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2161 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2221 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2281 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2341 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2401 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2461 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2521 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2581 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2641 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2701 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2761 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2821 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     2881 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     2941 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     3001 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     3061 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccattcgacg ctctccctta
     3121 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     3181 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     3241 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     3301 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     3361 acgatgcgtc cggcgtagag gatctggcta gcgatgaccc tgctgattgg ttcgctgacc
     3421 atttccgggt gcgggacggc gttaccagaa actcagaagg ttcgtccaac caaaccgact
     3481 ctgacggcag tttacgagag agatgatagg gtctgcttca gtaagccaga tgctacacaa
     3541 ttaggcttgt acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc
     3601 atacacatac gatttaggtg acactata
//
