LOCUS       40924_41017        3632 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Microarray spiking control vector pSP64bb2sp4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Sato M, Mitra RM, Coller J, Wang D, Spivey NW, Dewdney J, Denoux C, 
            Glazebrook J, Katagiri F.
  TITLE     A high-performance, small-scale microarray for expression profiling 
            of many samples in Arabidopsis-pathogen studies
  JOURNAL   Plant J. 49 (3), 565-577 (2007)
  PUBMED    17181774
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Sato M, Katagiri F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2006) Department of Plant Biology, University of 
            Minnesota, 1500 Gortner Avenue, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 3632)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2007"; volume: "49"; issue: "3"; pages: "565-577"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2006) Department of Plant Biology, University of Minnesota, 
            1500 Gortner Avenue, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..688
                     /label=transcribed region
                     /note="transcribed region"
     misc_feature    1..579
                     /label=backbone sequence 2 (bb2)
                     /note="backbone sequence 2 (bb2)"
     misc_feature    580..647
                     /label=spiking control 3 (sp3)
                     /note="spiking control 3 (sp3)"
     primer_bind     complement(702..718)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(726..742)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(750..780)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(795..816)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1104..1692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1866..2723)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2724..2828)
                     /label=AmpR promoter
     promoter        3616..3632
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatacaagc ttattggcat cgcggaacag tgttttcaga attgcgtgga taacactcat
       61 gtgaccgtat aaactcagtt gggctgcaag agggcaatgt tattacccag actctatctc
      121 cacatgacgc acgtgtctac gtcgtcacga ctaaagctaa tggtgcgaaa gctaactccc
      181 ttagtagacg caaatgctga cgtacaggtc tgcgaagtcg cgtggtatct tgtaccttgt
      241 acgcgtcgag tgaaggatag aatatctcaa cagttaagct ataggagatc gacggctctc
      301 aacgttccga tcaatagcat atacttgacc tcacaattgc tcccagtagg tcaaatcttg
      361 tgccgattgc accgttaggg acctacatct atagactggg ggacacagtc gatttaatgc
      421 tatgaacaat cgttcgagcc agaaccaggg aaccggctta gcatggttcc acaaggttat
      481 ttataggtcg cgcggagttt taaggttgca ggacttaagt aggtagacct aaatgcccta
      541 ccggctaccg attgatgttt accgactctt ttacccgggc cagaggaatt gttggtcacc
      601 gctgctaatg tgggacaaaa gggatatccc gccataatac ccagtctgag ctcccaaaaa
      661 aaaaaaaaaa aaaaaaaaaa aaaaaccgaa ttcgtaatca tgtcatagct gtttcctgtg
      721 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      781 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      841 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      901 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1861 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1981 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2821 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2881 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     2941 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     3001 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     3061 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
     3121 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3181 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3241 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3301 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3361 ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
     3421 gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc
     3481 gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac
     3541 acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg
     3601 tatcatacac atacgattta ggtgacacta ta
//
