LOCUS       40924_41012        3634 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Microarray spiking control vector pSP64bb2sp3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Sato M, Mitra RM, Coller J, Wang D, Spivey NW, Dewdney J, Denoux C, 
            Glazebrook J, Katagiri F.
  TITLE     A high-performance, small-scale microarray for expression profiling 
            of many samples in Arabidopsis-pathogen studies
  JOURNAL   Plant J. 49 (3), 565-577 (2007)
  PUBMED    17181774
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Sato M, Katagiri F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2006) Department of Plant Biology, University of 
            Minnesota, 1500 Gortner Avenue, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 3634)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3634)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2007"; volume: "49"; issue: "3"; pages: "565-577"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2006) Department of Plant Biology, University of Minnesota, 
            1500 Gortner Avenue, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..690
                     /label=transcribed region
                     /note="transcribed region"
     misc_feature    1..579
                     /label=backbone sequence 2 (bb2)
                     /note="backbone sequence 2 (bb2)"
     misc_feature    580..649
                     /label=spiking control 3 (sp3)
                     /note="spiking control 3 (sp3)"
     primer_bind     complement(704..720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(728..744)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(752..782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(797..818)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1106..1694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1868..2725)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2726..2830)
                     /label=AmpR promoter
     promoter        3618..3634
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatacaagc ttattggcat cgcggaacag tgttttcaga attgcgtgga taacactcat
       61 gtgaccgtat aaactcagtt gggctgcaag agggcaatgt tattacccag actctatctc
      121 cacatgacgc acgtgtctac gtcgtcacga ctaaagctaa tggtgcgaaa gctaactccc
      181 ttagtagacg caaatgctga cgtacaggtc tgcgaagtcg cgtggtatct tgtaccttgt
      241 acgcgtcgag tgaaggatag aatatctcaa cagttaagct ataggagatc gacggctctc
      301 aacgttccga tcaatagcat atacttgacc tcacaattgc tcccagtagg tcaaatcttg
      361 tgccgattgc accgttaggg acctacatct atagactggg ggacacagtc gatttaatgc
      421 tatgaacaat cgttcgagcc agaaccaggg aaccggctta gcatggttcc acaaggttat
      481 ttataggtcg cgcggagttt taaggttgca ggacttaagt aggtagacct aaatgcccta
      541 ccggctaccg attgatgttt accgactctt ttacccggga ttgcacaatt caacgcaagc
      601 tttttcagcc cagtattctt tgcgaactca gcagacagtg cgctcaggtg agctcccaaa
      661 aaaaaaaaaa aaaaaaaaaa aaaaaaaccg aattcgtaat catgtcatag ctgtttcctg
      721 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      781 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      841 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      901 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1741 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1801 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1861 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1921 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1981 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2041 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2101 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2161 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2221 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2281 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2341 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2401 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2461 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2521 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2581 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2641 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2701 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2761 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2821 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2881 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     2941 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3001 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     3061 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat tcgacgctct
     3121 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     3181 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     3241 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     3301 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     3361 ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct gattggttcg
     3421 ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg tccaaccaaa
     3481 ccgactctga cggcagttta cgagagagat gatagggtct gcttcagtaa gccagatgct
     3541 acacaattag gcttgtacat attgtcgtta gaacgcggct acaattaata cataacctta
     3601 tgtatcatac acatacgatt taggtgacac tata
//
