LOCUS       40924_41007        3635 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Microarray spiking control vector pSP64bb1sp1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Sato M, Mitra RM, Coller J, Wang D, Spivey NW, Dewdney J, Denoux C, 
            Glazebrook J, Katagiri F.
  TITLE     A high-performance, small-scale microarray for expression profiling 
            of many samples in Arabidopsis-pathogen studies
  JOURNAL   Plant J. 49 (3), 565-577 (2007)
  PUBMED    17181774
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Sato M, Katagiri F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2006) Department of Plant Biology, University of 
            Minnesota, 1500 Gortner Avenue, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 3635)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3635)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2007"; volume: "49"; issue: "3"; pages: "565-577"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2006) Department of Plant Biology, University of Minnesota, 
            1500 Gortner Avenue, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..691
                     /label=transcribed region
                     /note="transcribed region"
     misc_feature    1..584
                     /label=backbone sequence 1 (bb1)
                     /note="backbone sequence 1 (bb1)"
     misc_feature    585..650
                     /label=spiking control 1 (sp1)
                     /note="spiking control 1 (sp1)"
     primer_bind     complement(705..721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(729..745)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(753..783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(798..819)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1107..1695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1869..2726)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2727..2831)
                     /label=AmpR promoter
     promoter        3619..3635
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatacaagc ttattggcat cgcggaacag tgttttcaga attgcgtgga taacactcat
       61 gtgaccgtat aaactcagtt gggctgcaag agggcaatgt tattacccag actctatctc
      121 cacatgcgtg tacgcacgtg tctacgtcgt cacgactaaa gctaatggtg cgaaagctaa
      181 ctcccttagt agacgcaaat gctgacgtac aggtctgcga agtcgcgtgg tatcttgtac
      241 cttgtacgcg tcgagtgaag gatagaatat ctcaacagtt aagctatagg agatcgacgg
      301 ctctcaacgt tccgatcaat agcatatact tgacctcaca attgctccca gtaggtcaaa
      361 tcttgtgccg attgcaccgt tagggaccta catctataga ctgggggaca cagtcgattt
      421 aatgctatga acaatcgttc gagccagaac cagggaaccg gcttagcatg gttccacaag
      481 gttatttata ggtcgcgcgg agttttaagg ttgcaggact taagtaggta gacctaaatg
      541 ccctaccggc taccgattga tgtttaccga ctcttttacc cgggatggcc cggaataggg
      601 ccactgactg gacgttgctt cggtccaata cactatacta aacggagccc gagctcccaa
      661 aaaaaaaaaa aaaaaaaaaa aaaaaaaacc gaattcgtaa tcatgtcata gctgtttcct
      721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      781 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      961 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1021 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1081 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1141 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1561 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1621 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1681 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1741 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1801 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1861 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1921 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1981 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2041 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2101 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2161 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2221 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2281 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2341 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2401 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2461 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2521 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2581 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2641 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2701 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2761 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2821 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2881 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     2941 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     3001 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     3061 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca ttcgacgctc
     3121 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     3181 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     3241 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     3301 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     3361 gccggccacg atgcgtccgg cgtagaggat ctggctagcg atgaccctgc tgattggttc
     3421 gctgaccatt tccgggtgcg ggacggcgtt accagaaact cagaaggttc gtccaaccaa
     3481 accgactctg acggcagttt acgagagaga tgatagggtc tgcttcagta agccagatgc
     3541 tacacaatta ggcttgtaca tattgtcgtt agaacgcggc tacaattaat acataacctt
     3601 atgtatcata cacatacgat ttaggtgaca ctata
//
