LOCUS       V016670                 8784 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016670
VERSION     V016670
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3804..3820)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3828..3844)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3852..3882)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3897..3918)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4206..4794)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4968..5825)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5826..5930)
                     /label="AmpR promoter"
     CDS             complement(6313..6960)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
ORIGIN
        1 caagatttac acgctatcca gcttcaatta gaagaagaga tgtttaatgg tggcattcgt
       61 cgcttcgaag cagatcaaca acgccagatt gcagcaggta gcgagagcga cacagcatgg
      121 aaccgccgcc tgttgtcaga acttattgca cctatggctg aaggcattca ggcttataaa
      181 gaagagtacg aaggtaagaa aggtcgtgca cctcgcgcat tggctttctt acaatgtgta
      241 gaaaatgaag ttgcagcata catcactatg aaagttgtta tggatatgct gaatacggat
      301 gctacccttc aggctattgc aatgagtgta gcagaacgca ttgaagacca agtgcgcttt
      361 tctaagctag aaggtcacgc cgctaaatac tttgagaagg ttaagaagtc actcaaggct
      421 agccgtacta agtcatatcg tcacgctcat aacgtagctg tagttgctga aaaatcagtt
      481 gcagaaaagg acgcggactt tgaccgttgg gaggcgtggc caaaagaaac tcaattgcag
      541 attggtacta ccttgcttga aatcttagaa ggtagcgttt tctataatgg tgaacctgta
      601 tttatgcgtg ctatgcgcac ttatggcgga aagactattt actacttaca aacttctgaa
      661 agtgtaggcc agtggattag cgcattcaaa gagcacgtag cgcaattaag cccagcttat
      721 gccccttgcg taatccctcc tcgtccttgg agaactccat ttaatggagg gttccatact
      781 gagaaggtag ctagccgtat ccgtcttgta aaaggtaacc gtgagcatgt acgcaagttg
      841 actcaaaagc aaatgccaaa ggtttataag gctatcaacg cattacaaaa tacacaatgg
      901 caaatcaaca aggatgtatt agcagttatt gaagaagtaa tccgcttaga ccttggttat
      961 ggtgtacctt ccttcaagcc actgattgac aaggagaaca agccagctaa cccggtacct
     1021 gttgaattcc aacacctgcg cggtcgtgaa ctgaaagaga tgctatcacc tgagcagtgg
     1081 caacaattca ttaactggaa aggcgaatgc gcgcgcctat ataccgcaga aactaagcgc
     1141 ggttcaaagt ccgccgccgt tgttcgcatg gtaggacagg cccgtaaata tagcgccttt
     1201 gaatccattt acttcgtgta cgcaatggat agccgcagcc gtgtctatgt gcaatctagc
     1261 acgctctctc cgcagtctaa cgacttaggt aaggcattac tccgctttac cgagggacgc
     1321 cctgtgaatg gcgtagaagc gcttaaatgg ttctgcatca atggtgctaa cctttgggga
     1381 tgggacaaga aaacttttga tgtgcgcgtg tctaacgtat tagatgagga attccaagat
     1441 atgtgtcgag acatcgccgc agaccctctc acattcaccc aatgggctaa agctgatgca
     1501 ccttatgaat tcctcgcttg gtgctttgag tatgctcaat accttgattt ggtggatgaa
     1561 ggaagggccg acgaattccg cactcaccta ccagtacatc aggacgggtc ttgttcaggc
     1621 attcagcact atagtgctat gcttcgcgac gaagtagggg ccaaagctgt taacctgaaa
     1681 ccctccgatg caccgcagga tatctatggg gcggtggcgc aagtggttat caagaagaat
     1741 gcgctatata tggatgcgga cgatgcaacc acgtttactt ctggtagcgt cacgctgtcc
     1801 ggtacagaac tgcgagcaat ggctagcgca tgggatagta ttggtattac ccgtagctta
     1861 accaaaaagc ccgtgatgac cttgccatat ggttctactc gcttaacttg ccgtgaatct
     1921 gtgattgatt acatcgtaga cttagaggaa aaagaggcgc agaaggcagt agcagaaggg
     1981 cggacggcaa acaaggtaca tccttttgaa gacgatcgtc aagattactt gactccgggc
     2041 gcagcttaca actacatgac ggcactaatc tggccttcta tttctgaagt agttaaggca
     2101 ccgatagtag ctatgaagat gatacgccag cttgcacgct ttgcagcgaa acgtaatgaa
     2161 ggcctgatgt acaccctgcc tactggcttc atcttagaac agaagatcat ggcaaccgag
     2221 atgctacgcg tgcgtacctg tctgatgggt gatatcaaga tgtcccttca ggttgaaacg
     2281 gatatcgtag atgaagccgc tatgatggga gcagcagcac ctaatttcgt acacggtcat
     2341 gacgcaagtc accttatcct taccgtatgt gaattggtag acaagggcgt aactagtatc
     2401 gctgtaatcc acgactcttt tggtactcat gcagacaaca ccctcactct tagagtggca
     2461 cttaaagggc agatggttgc aatgtatatt gatggtaatg cgcttcagaa actactggag
     2521 gagcatgaag agcgctggat ggttgataca ggtatcgaag tacctgagca aggggagttc
     2581 gaccttaacg aaatcatgga ttctgaatac gtatttgcct aaggatcctc tagagtcatt
     2641 tatattcaac tgttaactaa aactgtctgc aaattaatca atgtctctag taaaattccg
     2701 attttatacc cactcctatt ttcatcaata ggacatgata taataaaaaa gaacggagat
     2761 tgaggcctcc gttctcttgg ttgtacttgt tgcaatagta cgtttatttg gttacgtata
     2821 ttatagcaag tactccaaca aaaaatcaat cgttggagga atttagaaat gaatggaatt
     2881 atcaaaacaa taaaaacaac gcaatatgct caaatacata acgcgccatt acaaacggac
     2941 ctggaggact tgcggtctat cggtctatta agtcatatta tgagcttagg tgaaggttgg
     3001 acgattagga agacccagtt acaaaacaaa ttttctcgta gaaatgtaga cgctgcttgg
     3061 aaggaattag ctattaaaca atacgctgtc ggttttagcg catatgttga cggaaaaaaa
     3121 gattattttt acgccgtatc agatattccg atttcgcaag ctaatttcga attgcttgtc
     3181 attgaacaaa taaatctctt acaatcgcaa ggtaaaaacg tcatgacgtt aagcgtgata
     3241 caacattcgc cacttgaaat cactgaaaaa atatctgatg tacaaaatgt acatcagata
     3301 caaaataagc aaatttcttc tgatgtacgt tctgtacaac acagtgtgta caacacagaa
     3361 cgtacaccta taaataaaaa agaaacaaat gaaaaattaa caaatgaaaa aattagtagt
     3421 agtccacgtg attttattga tgataaatta cgagaaaaat ataataatgt acctttcgac
     3481 gaagtgaaaa gtgaaatgct taacgatact gtaatcgttg atacgaataa gcaatataaa
     3541 tcactattag aataccgtct taaacattgg aaaccgaagc aaacaaaaag gaaacgtgta
     3601 gtacgtaatg cacgtaaaga aatggttcca aagtggctac aaacaaatga tgaaaatgca
     3661 gtagcagaag atcttccagt gaataatgat tttgaagctg aaaaggaaaa aatgaaggcg
     3721 gagcttatgc aattagatgc agaattaaaa gcaggtaatc gttaataaat gacctgcagg
     3781 catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3841 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3901 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4021 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4081 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4141 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4621 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4741 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4801 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4861 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4921 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4981 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5041 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5101 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5161 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5221 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5281 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5341 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5401 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5461 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5521 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5581 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5641 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5701 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5761 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5821 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5941 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     6001 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     6061 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     6121 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     6181 tcagagcaga ttgtactgag agtgcaccat agatctggag ctgtaatata aaaaccttct
     6241 tcaactaacg gggcaggtta gtgacattag aaaaccgact gtaaaaagta cagtcggcat
     6301 tatctcatat tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat
     6361 aaacaatcct gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa
     6421 atatattgaa ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt
     6481 actattatta ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag
     6541 agaaaaagca ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt
     6601 ctctacatca gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca
     6661 aataccagag aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc
     6721 ccaataacct aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat
     6781 cacccttgtc actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt
     6841 tcggtataaa acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa
     6901 ataatgatta aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat
     6961 ttgatatgcc tcctaaattt ttatctaaag tgaatttagg aggcttactt gtctgctttc
     7021 ttcattagaa tcaatccttt tttaaaagtc aatattactg taacataaat atatatttta
     7081 aaaatatccc actttatcca attttcgttt gttgaactaa tgggtgcttt agttgaagaa
     7141 taaaagacca cattaaaaaa tgtggtcttt tgtgtttttt taaaggattt gagcgtagcg
     7201 aaaaatcctt ttctttctta tcttgataat aagggtaact attgccggat cgtcctcagg
     7261 agtaggcgac atcgctaaat aatgatctat gcggtgtgaa ataccgcaca gatgcgtaag
     7321 gagaaaatac cgcatcaggc gccctttgcg ttcacttaac taacttatag gggtaacact
     7381 taaaaaagaa tcaataacga tagaaaccgc tcctaaagca ggtgcatttt ttcctaacga
     7441 agaaggcaat agttcacatt tattgtctaa atgagaatgg actctagaag aaacttcgtt
     7501 tttaatcgta tttaaaacaa tgggatgaga ttcaattata tgatttctca agataacagc
     7561 ttctatatca aatgtattaa ggatattggt taatccaatt ccgatataaa agccaaagtt
     7621 ttgaagtgca tttaacattt ctacatcatt tttatttgcg cgttccacaa tctcttttcg
     7681 agaaatattc ttttcttctt tagagagcga agccagtaac gctttttcag aagcatataa
     7741 ttcccaacag cctcgatttc cacagctgca tttgggtcca ttaaaatcta tcgtcatatg
     7801 acccatttcc ccagaaaaac cctgaacacc tttatacaat tcgttgttaa taacaagtcc
     7861 agttccaatt ccgatattaa tactgatgta aacgatgttt tcatagtttt ttgtcatacc
     7921 aaatactttt tcaccgtatg ctcctgcatt agcttcattt tcaacaaaaa ccggaacatt
     7981 aaactcactc tcaattaaaa actgcaaatc tttgatattc caatttaagt taggcatgaa
     8041 aataatttgc tgatgacgat ctacaaggcc tggaacacaa attcctattc cgactagacc
     8101 ataaggggac tcaggcatat gggttacaaa accatgaata agtgcaaata aaatctcttt
     8161 tacttcacta gcggaagaac tagacaagtc agaagtcttc tcgagaataa tatttccttc
     8221 taagtcggtt agaattccgt taagatagtc gactcctata tcaataccaa tcgagtagcc
     8281 tgcattctta ttaaaaacaa gcattacagg tcttctgccg cctctagatt gccctgcccc
     8341 aatttcaaaa ataaaatctt tttcaagcag tgtatttact tgagaggaga cagtagactt
     8401 gtttaatcct gtaatctcag agagagttgc cctggagaca ggggagttct tcaaaatttc
     8461 atctaatatt aatttttgat tcattttttt tactaaagct tgatctgcaa tttgaataat
     8521 aaccactcct ttgtttatcc accgaactaa gttggtgttt tttgaagctt gaattagata
     8581 tttaaaagta tcatatctaa tattataact aaattttcta aaaaaaacat tgaaataaac
     8641 atttattttg tatatgatga gataaagtta gtttattgga taaacaaact aactcaatta
     8701 agatagttga tggataaact tgttcactta aatcaaaggg ggaaatgaca aatggtccaa
     8761 actagttcga agatctccgg agct
//
