LOCUS       40924_40997        4103 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSP-luc+NF, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Brondyk WH, Groskreutz DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1996) R
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Kenefick K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2000) Technical Writing, Promega Corporation, 2800
            Woods Hollow Road, Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4103)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4103)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-JAN-1996) R"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2000) Technical Writing, Promega Corporation, 2800 Woods 
            Hollow Road, Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On May 9, 2000 this sequence version replaced U47123.1.
FEATURES             Location/Qualifiers
     source          1..4103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1
                     /label=SP6 RNA polymerase initiation site
                     /note="SP6 RNA polymerase initiation site"
     misc_feature    11..53
                     /label=upstream multiple cloning site
                     /note="upstream multiple cloning site"
     CDS             54..1697
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="DAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHI
                     EVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPAN
                     DIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMY
                     TFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARD
                     PIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQ
                     SALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGL
                     TETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGY
                     VNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQ
                     HPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFV
                     DEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    1702..1731
                     /label=downstream multiple cloning site
                     /note="downstream multiple cloning site"
     promoter        complement(1741..1759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2014
                     /label=ColE1-derived replication origin
                     /note="ColE1-derived replication origin"
     rep_origin      complement(2017..2605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2779..3636)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3637..3741)
                     /label=AmpR promoter
     promoter        4087..4103
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaaccagatc ggtaccgcta gcaagatctc ctaggaagct ttccatggtc accgacgcca
       61 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
      121 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
      181 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
      241 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
      301 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
      361 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
      421 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
      481 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
      541 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
      601 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
      661 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
      721 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
      781 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
      841 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
      901 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
      961 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     1021 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     1081 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     1141 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     1201 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     1261 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     1321 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     1381 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     1441 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     1501 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     1561 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     1621 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     1681 gcggaaagat cgccgtgtaa ttctagagaa ttcgatatca tcgatctcga ggccggtctc
     1741 cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat gaatcggcca
     1801 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1861 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1921 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1981 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2041 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2101 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2161 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2221 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2281 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2341 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2401 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2461 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2521 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2581 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2641 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2701 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2761 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2821 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2881 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2941 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3001 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3061 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3121 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3181 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3241 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3301 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3361 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3421 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3481 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3541 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3601 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3661 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3721 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     3781 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     3841 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     3901 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     3961 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     4021 tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca
     4081 catacgattt aggtgacact ata
//
