LOCUS       40924_40967       11259 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSOS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Chang C, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 11259)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11259)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3326..3342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3549..3736
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      complement(4120..4708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4882..5739)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(6498..7589)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7590..7995)
                     /label=LEU2 promoter
     protein_bind    complement(8833..8880)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        10552..11257
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 aagcttctag acccgggggc accatgcagg cgcagcagct gccctacgag tttttcagcg
       61 aagagaacgc gcccaagtgg cggggactac tggtgcctgc gctgaaaaag gtccaggggc
      121 aagttcatcc tactctcgag tctaatgatg atgctcttca gtatgttgaa gaattaattt
      181 tgcaattatt aaatatgcta tgccaagctc agccccgaag tgcttcagat gtagaggaac
      241 gtgttcaaaa aagtttccct catccaattg ataaatgggc aatagctgat gcccaatcag
      301 ctattgaaaa gaggaagcga agaaaccctt tatctctccc agtagaaaaa attcatcctt
      361 tattaaagga ggtcctaggt tataaaattg accaccaggt ttctgtttac atagtagcag
      421 tcttagaata catttctgca gacattttaa agctggttgg gaattatgta agaaatatac
      481 ggcattatga aattacaaaa caagatatta aagtggcaat gtgtgctgac aaggtattga
      541 tggatatgtt tcatcaagat gtagaagata ttaatatatt atctttaact gacgaagagc
      601 cttccacctc aggagaacaa acttactatg atttggtaaa agcatttatg gcagaaattc
      661 gacaatatat aagggaacta aatctaatta taaaagtttt tagagagccc tttgtctcca
      721 attcaaaatt gttttcagct aatgatgtag aaaatatatt tagtcgcata gtagatatac
      781 atgaacttag tgtaaagtta ctgggccata tagaagatac agtagaaatg acagatgaag
      841 gcagtcccca tccactagta ggaagctgct ttgaagactt agcagaggaa ctggcatttg
      901 atccatatga atcgtatgct cgagatattt tgcgacctgg ttttcatgat cgtttcctta
      961 gtcagttatc aaagcctggg gcagcacttt atttgcagtc aataggcgaa ggtttcaaag
     1021 aagctgttca atatgtttta cccaggctgc ttctggcccc tgtttaccac tgtctccatt
     1081 actttgaact tttgaagcag ttagaagaaa aaagtgaaga tcaagaagac aaggaatgtt
     1141 taaaacaagc aataacagct ttgcttaatg ttcagagtgg tatggaaaaa atatgttcta
     1201 aaagtcttgc aaaacgaaga ctgagtgaat ctgcatgtcg gttttatagt cagcaaatga
     1261 aggggaaaca actagcaatc aagaagatga acgagattca gaagaatatt gatggttggg
     1321 agggaaaaga cattggacag tgttgtaatg aatttataat ggaaggaact cttacacgtg
     1381 taggagccaa acatgagaga cacatatttc tctttgatgg cttaatgatt tgctgtaaat
     1441 caaatcatgg gcagccaaga cttcctggtg ctagcaatgc agaatatcgt cttaaagaaa
     1501 agttttttat gcgaaaggta caaattaatg ataaagatga caccaatgaa tacaagcatg
     1561 cttttgaaat aattttaaaa gatgaaaata gtgttatatt ttctgccaag tcagctgaag
     1621 agaaaaacaa ttggatggca gcattgatat ctttacagta ccggagtaca ctggaaagga
     1681 tgcttgatgt aacaatgcta caggaagaga aagaggagca gatgaggctg cctagtgctg
     1741 atgtttatag atttgcagag cctgactctg aagagaatat tatatttgaa gagaacatgc
     1801 agcccaaggc tggaattcca attatcaaag caggaactgt tattaaactt atagagaggc
     1861 ttacgtacca tatgtacgca gatcccaatt ttgttcggac atttcttaca acatacagat
     1921 ccttttgcaa acctcaagaa ctactgagtc ttataataga aaggtttgaa attccagagc
     1981 ctgagccaac agaagctgat cgcatagcta tagagaatgg agatcaaccc ttgagtgcag
     2041 aactgaaaag atttagaaaa gaatatatac agcctgtgca actgcgagta ttaaatgtat
     2101 gtcggcactg ggtagagcac cacttctatg attttgaaag agatgcatat cttttgcaac
     2161 gaatggaaga atttattgga acagtaagag gtaaagcaat gaaaaaatgg gttgaatcca
     2221 tcactaaaat aatccaaagg aaaaaaattg caagagacaa tggaccaggt cataatatta
     2281 catttcagag ttcacctccc acagttgagt ggcatataag cagacctggg cacatagaga
     2341 cttttgacct gctcacctta cacccaatag aaattgctcg acaactcact ttacttgaat
     2401 cagatctata ccgagctgta cagccatcag aattagttgg aagtgtgtgg acaaaagaag
     2461 acaaagaaat taactctcct aatcttctga aaatgattcg acataccacc aacctcactc
     2521 tgtggtttga gaaatgtatt gtagaaactg aaaatttaga agaaagagta gctgtggtga
     2581 gtcgaattat tgagattcta caagtctttc aagagttgaa caactttaat ggtgtccttg
     2641 aggttgtcag tgctatgaat tcatcacctg tttacagact agaccacaca tttgagcaaa
     2701 taccaagtcg ccagaagaaa attttagaag aagctcatga attgagtgaa gatcactata
     2761 agaaatattt ggcaaaactc aggtctatta atccaccatg tgtgcctttc tttggaattt
     2821 atctcactaa tatcttgaaa acagaagaag gcaaccctga ggtcctaaaa agacatggaa
     2881 aagagcttat aaactttagc aaaaggagga aagtagcaga aataacagga gagatccagc
     2941 agtaccaaaa tcagccttac tgtttacgag tagaatcaga tatcaaaagg ttctttgaaa
     3001 acttgaatcc gatgggaaat agcatggaga aggaatttac agattatctt ttcaacaaat
     3061 ccctagaaat agaaccacga aaccctaagc ctctcccaag atttccaaaa aaatatagct
     3121 atcccctaaa atctcctggt gttcgtccat caaacccaag accaggtacc atgaggcatc
     3181 ccacacctct gcagcaggag ccaaggaaaa ttagttatag taggatcccc atggcccggg
     3241 cgacgtcgac gcgcgcacgc gtgagctcgc ggccgccgcg gttaattaat taattaaccg
     3301 cggccttttt ggccagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3361 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agagctttgg
     3421 acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc
     3481 cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt gttgacactt
     3541 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
     3601 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
     3661 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg
     3721 accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat ttcacccaat
     3781 tgtagatatg ctaactccag caatgagttg atgaatctcg gtgtgtattt tatgtcctca
     3841 gaggacaaca cctgttgtaa tcgttcttcc acacggatcg atcctgcatt aatgaatcgg
     3901 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3961 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4021 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4081 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4141 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4201 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4261 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4321 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4381 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4441 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4501 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4561 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4621 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4681 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4741 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4801 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4861 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4921 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4981 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5041 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5101 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5161 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5221 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5281 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5341 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5401 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5461 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5521 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5581 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5641 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5701 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatgatc
     5761 tattacatta tgggtggtat gttggaataa aaatcaacta tcatctacta actagtattt
     5821 acgttactag tatattatca tatacggtgt tagaagatga cgcaaatgat gagaaatagt
     5881 catctaaatt agtggaagct gaaacgcaag gattgataat gtaataggat caatgaatat
     5941 taacatataa aatgatgata ataatattta tagaattgtg tagaattgca gattcccttt
     6001 tatggattcc taaatcctcg actacgtcgt taaggccgtt tctgacagag taaaattctt
     6061 gagggaactt tcaccattat gggaaatggt tcaagaaggt attgacttaa actccatcaa
     6121 atggtcaggt cattgagtgt tttttatttg ttgtattttt ttttttttag agaaaatcct
     6181 ccaatatata aattaggaat catagtttca tgattttctg ttacacctaa ctttttgtgt
     6241 ggtgccctcc tccttgtcaa tattaatgtt aaagtgcaat tctttttcct tatcacgttg
     6301 agccattagt atcaatttgc ttacctgtat tcctttacat cctccttttt ctccttcttg
     6361 ataaatgtat gtagattgcg tatatagttt cgtctaccct atgaacatat tccattttgt
     6421 aatttcgtgt cgtttctatt atgaatttca tttataaagt ttatgtacaa atatcataaa
     6481 aaaagagaat ctttttaagc aaggattttc ttaacttctt cggcgacagc atcaccgact
     6541 tcggtggtac tgttggaacc acctaaatca ccagttctga tacctgcatc caaaaccttt
     6601 ttaactgcat cttcaatggc cttaccttct tcaggcaagt tcaatgacaa tttcaacatc
     6661 attgcagcag acaagatagt ggcgataggg ttgaccttat tctttggcaa atctggagca
     6721 gaaccgtggc atggttcgta caaaccaaat gcggtgttct tgtctggcaa agaggccaag
     6781 gacgcagatg gcaacaaacc caaggaacct gggataacgg aggcttcatc ggagatgata
     6841 tcaccaaaca tgttgctggt gattataata ccatttaggt gggttgggtt cttaactagg
     6901 atcatggcgg cagaatcaat caattgatgt tgaaccttca atgtagggaa ttcgttcttg
     6961 atggtttcct ccacagtttt tctccataat cttgaagagg ccaaaacatt agctttatcc
     7021 aaggaccaaa taggcaatgg tggctcatgt tgtagggcca tgaaagcggc cattcttgtg
     7081 attctttgca cttctggaac ggtgtattgt tcactatccc aagcgacacc atcaccatcg
     7141 tcttcctttc tcttaccaaa gtaaatacct cccactaatt ctctgacaac aacgaagtca
     7201 gtacctttag caaattgtgg cttgattgga gataagtcta aaagagagtc ggatgcaaag
     7261 ttacatggtc ttaagttggc gtacaattga agttctttac ggatttttag taaaccttgt
     7321 tcaggtctaa cactaccggt accccattta ggaccaccca cagcacctaa caaaacggca
     7381 tcagccttct tggaggcttc cagcgcctca tctggaagtg gaacacctgt agcatcgata
     7441 gcagcaccac caattaaatg attttcgaaa tcgaacttga cattggaacg aacatcagaa
     7501 atagctttaa gaaccttaat ggcttcggct gtgatttctt gaccaacgtg gtcacctggc
     7561 aaaacgacga tcttcttagg ggcagacatt agaatggtat atccttgaaa tatatatata
     7621 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     7681 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     7741 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcgctc tcacctttcc
     7801 tttttctccc aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc tgaaattaac
     7861 aaaaaatttc cagtcatcga atttgattct gtgcgatagc gcccctgtgt gttctcgtta
     7921 tgttgaggaa aaaaataatg gttgctaaga gattcgaact cttgcatctt acgatacctg
     7981 agtattccca cagttaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
     8041 agagcgctaa tttttcaaac aaagaatcta gctgcatttt tacagaacag aaatgcaacg
     8101 cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat
     8161 cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc
     8221 tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg
     8281 ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac
     8341 tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc
     8401 tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc
     8461 attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa
     8521 atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt
     8581 ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca
     8641 agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata
     8701 gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc
     8761 gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt
     8821 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc
     8881 aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc
     8941 actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac
     9001 ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa
     9061 aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct
     9121 tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc
     9181 ttcaatgcta tcatttcctt tgatattgga tcatatgcat agtaccgaga aactagtgcg
     9241 aagtagtgat caggtattgc tgttatctga tgagtatacg ttgtcctggc cacggcagaa
     9301 gcacgcttat cgctccaatt tcccacaaca ttagtcaact ccgttaggcc cttcattgaa
     9361 agaaatgagg tcatcaaatg tcttccaatg tgagattttg ggccattttt tatagcaaag
     9421 attgaataag gcgcattttt cttcaaagct agctttaatg cggtagttta tcacagttaa
     9481 attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg
     9541 gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct
     9601 tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat
     9661 atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc
     9721 gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga
     9781 ccacacccgt cctgtggatg atccgggatc gaagaaatga tggtaaatga aataggaaat
     9841 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg
     9901 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc
     9961 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg
    10021 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc
    10081 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact
    10141 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga
    10201 agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat agagcgacca
    10261 tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa acagcaatag
    10321 ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg tctgtttgag
    10381 tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa gggaacttta
    10441 cacttctcct atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca
    10501 cccctccgcg ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg
    10561 ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg
    10621 attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa
    10681 tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat tacactgcct
    10741 cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat
    10801 atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc
    10861 aacttctttt cttttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca
    10921 atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac
    10981 agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc
    11041 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact
    11101 tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt
    11161 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg
    11221 cacaatattt caagctatac caagcataca atcaactcc
//
