LOCUS       40924_40777        9115 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSOL9DEF1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Weinhold A, Wielsch N, Svato A, Baldwin IT.
  TITLE     Label-free nanoUPLC-MS E based quantification of antimicrobial 
            peptides from the leaf apoplast of Nicotiana attenuata
  JOURNAL   BMC Plant Biol. 15 (1), 18 (2015)
  PUBMED    25604123
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   Gase K, Weinhold A, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2013) Molecular Ecology, Max-Planck-Institute for 
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, 
            Germany
REFERENCE   3  (bases 1 to 9115)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9115)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2015"; volume: "15"; issue: "1"; pages: "18"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2013) Molecular Ecology, Max-Planck-Institute for Chemical 
            Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    244..268
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(342..594)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(619..1641)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(1662..1845)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2155..2500
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2521..2841
                     /codon_start=1
                     /product="DEF1"
                     /label=DEF1
                     /note="defensin 1; from Nicotiana attenuata; NaDEF1"
                     /protein_id="AHZ55764.1"
                     /translation="MARSLCFMAFAVLAMMLFVAYEVQAKSTCKAESNTFEGFCVTKPP
                     CRRACLKEKFTDGKCSKILRRCICYKPCVFDGKMINTGAETLAEEANTLAEALLEEEMM
                     DN"
     polyA_signal    2856..3030
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    3102..3126
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3483..4071
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      complement(4514..4708)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4777..5847)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6279..6905)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8315..9106
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 gaattcgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
       61 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
      121 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
      181 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
      241 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
      301 gcggacgttt ttaatgtact ggcgcgccgg atccaattcc cgatctagta acatagatga
      361 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
      421 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
      481 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
      541 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa
      601 attcgagctg gtaccctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact
      661 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg
      721 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca
      781 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg
      841 gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta
      901 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc
      961 gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt
     1021 gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca
     1081 gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc
     1141 gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg
     1201 ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct
     1261 aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag
     1321 ttcggtttca ggcaggtctt gcaacgtgac accctgtgaa cggcgggaga tgcaataggt
     1381 caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     1441 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     1501 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     1561 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     1621 ggtgagttca ggctttttca tgcgaaacga tccagatccg gtgcagatta tttggattga
     1681 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     1741 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     1801 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc
     1861 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     1921 ggggtcataa cgtgactccc ttaattctcc gctcatgatc ctctagagct tcatggagtc
     1981 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
     2041 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
     2101 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
     2161 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
     2221 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
     2281 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     2341 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     2401 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     2461 ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc ttgactcgag
     2521 atggctcgct ccttgtgctt catggcattt gcagtcttgg caatgatgct ttttgttgcc
     2581 tatgaggtgc aagctaagag tacttgcaaa gcagaaagca atacattcga gggattctgc
     2641 gttaccaaac caccatgcag aagagcttgt ctcaaggaga aatttaccga tggaaaatgt
     2701 agcaaaatcc tcagaaggtg catttgctat aagccatgtg tatttgatgg gaagatgatc
     2761 aatacaggag ctgaaacttt agccgaggag gcaaacactt tggctgaagc tttgcttgaa
     2821 gaagagatga tggataacta aggtgaccgg tcgagtttct ccataataat gtgtgagtag
     2881 ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga
     2941 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     3001 aaaccaaaat ccagtactaa aatccagatc ccccgaatta atgagcttgg cgcgccggat
     3061 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     3121 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     3181 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc agatccgtag
     3241 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3301 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3361 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3421 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3481 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3541 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3601 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3661 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3721 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3781 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3841 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3901 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3961 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4021 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4081 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtacactccg
     4141 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     4201 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4261 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     4321 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     4381 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     4441 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     4501 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     4561 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     4621 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     4681 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     4741 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     4801 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     4861 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     4921 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     4981 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     5041 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     5101 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     5161 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     5221 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     5281 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     5341 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     5401 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     5461 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     5521 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     5581 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     5641 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     5701 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     5761 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     5821 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     5881 ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     5941 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
     6001 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
     6061 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
     6121 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
     6181 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
     6241 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
     6301 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
     6361 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
     6421 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
     6481 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
     6541 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
     6601 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
     6661 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
     6721 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
     6781 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
     6841 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
     6901 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
     6961 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
     7021 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
     7081 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
     7141 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
     7201 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
     7261 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
     7321 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
     7381 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
     7441 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
     7501 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
     7561 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
     7621 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
     7681 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
     7741 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
     7801 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
     7861 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
     7921 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
     7981 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
     8041 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
     8101 aggggaaccc tgtggttggc atgcataatt gtggtttcaa aatcggctcc gtcgatacta
     8161 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     8221 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     8281 ttaaatactg tagaaaagag gaaggaaata ataaatgatt gaacaagatg gattgcacgc
     8341 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     8401 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     8461 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     8521 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     8581 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     8641 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     8701 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     8761 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     8821 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     8881 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     8941 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     9001 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     9061 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctagt accta
//
