LOCUS       40924_40772        9182 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSOL9CAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Gase K, Weinhold A, Bozorov T, Schuck S, Baldwin IT.
  TITLE     Efficient screening of transgenic plant lines for ecological 
            research
  JOURNAL   Mol Ecol Resour 11 (5), 890-902 (2011)
  PUBMED    21518300
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   Gase K, Weinhold A, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2010) Molecular Ecology, Max-Planck-Institute for 
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena 07745, Germany
REFERENCE   3  (bases 1 to 9182)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9182)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ecol 
            Resour"; date: "2011"; volume: "11"; issue: "5"; pages: "890-902"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2010) Molecular Ecology, Max-Planck-Institute for Chemical 
            Ecology, Hans-Knoell-Strasse 8, Jena 07745, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    244..268
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(342..594)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(619..1641)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(1662..1845)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2155..2500
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2521..2904
                     /codon_start=1
                     /product="antimicrobial peptide"
                     /label=antimicrobial peptide
                     /note="from Capsella bursa-pastoris"
                     /protein_id="AEJ22726.1"
                     /translation="MASKTLILLGLFAILLVVSEVSAARESGMVKPESEETVQPEGYGG
                     HGGHGGHGGHGGHGGHGHGGGGHGLDGYHGGHGGHGGGSYGGGGYGGGGYGGGGHGGGY
                     NGGGHHGGGGHGLNEPVQTQPGV"
     regulatory      2682..2691
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2923..3097
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    3169..3193
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3550..4138
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      complement(4581..4775)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4844..5914)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6346..6972)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8382..9173
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gaattcgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
       61 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
      121 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
      181 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
      241 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
      301 gcggacgttt ttaatgtact ggcgcgccgg atccaattcc cgatctagta acatagatga
      361 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
      421 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
      481 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
      541 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa
      601 attcgagctg gtaccctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact
      661 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg
      721 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca
      781 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg
      841 gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta
      901 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc
      961 gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt
     1021 gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca
     1081 gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc
     1141 gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg
     1201 ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct
     1261 aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag
     1321 ttcggtttca ggcaggtctt gcaacgtgac accctgtgaa cggcgggaga tgcaataggt
     1381 caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     1441 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     1501 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     1561 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     1621 ggtgagttca ggctttttca tgcgaaacga tccagatccg gtgcagatta tttggattga
     1681 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     1741 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     1801 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc
     1861 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     1921 ggggtcataa cgtgactccc ttaattctcc gctcatgatc ctctagagct tcatggagtc
     1981 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
     2041 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
     2101 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
     2161 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
     2221 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
     2281 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     2341 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     2401 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     2461 ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc ttgactcgag
     2521 atggcttcca agactttgat actgttgggt ctctttgcaa ttcttctcgt tgtctccgaa
     2581 gtgtctgccg caagagagtc cggcatggtg aagccggaga gtgaggaaac agtgcaacct
     2641 gaaggttatg gcggccacgg cggccatggt ggtcatggag gccatggtgg ccacggaggt
     2701 cacggacatg gaggaggagg ccatggactt gacggatacc acggaggaca tggtggtcac
     2761 ggaggaggaa gttacggagg aggtggttac ggaggcggtg gttacggagg aggtggtcac
     2821 ggaggaggat acaacggagg aggacaccac ggaggaggag gccacgggct taatgaacct
     2881 gttcagactc agcccggtgt ttaagcttgg tgaccggtcg agtttctcca taataatgtg
     2941 tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca
     3001 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     3061 attcctaaaa ccaaaatcca gtactaaaat ccagatcccc cgaattaatg agcttggcgc
     3121 gccggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     3181 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     3241 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga
     3301 tccgtagagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3361 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3421 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3481 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3661 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3781 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3841 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3901 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3961 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4021 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4081 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4141 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgta
     4201 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     4261 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4321 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     4381 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     4441 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     4501 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     4561 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     4621 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     4681 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     4741 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     4801 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     4861 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     4921 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     4981 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     5041 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     5101 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     5161 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     5221 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     5281 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     5341 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     5401 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     5461 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     5521 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     5581 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     5641 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     5701 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
     5761 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
     5821 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
     5881 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
     5941 agtgccattg cagggccggc aggcaaccca gccgcttacg cctggccaac cgcccgttcc
     6001 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
     6061 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
     6121 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
     6181 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
     6241 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
     6301 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
     6361 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
     6421 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
     6481 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
     6541 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
     6601 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
     6661 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
     6721 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
     6781 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
     6841 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
     6901 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
     6961 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
     7021 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
     7081 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
     7141 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
     7201 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
     7261 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
     7321 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
     7381 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
     7441 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
     7501 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
     7561 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
     7621 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
     7681 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
     7741 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
     7801 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
     7861 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
     7921 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
     7981 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
     8041 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
     8101 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
     8161 gatcccgagg ggaaccctgt ggttggcatg cataattgtg gtttcaaaat cggctccgtc
     8221 gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta
     8281 aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg
     8341 ggtatcttta aatactgtag aaaagaggaa ggaaataata aatgattgaa caagatggat
     8401 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     8461 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     8521 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     8581 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     8641 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     8701 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     8761 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     8821 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     8881 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     8941 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     9001 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     9061 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     9121 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctagtacc
     9181 ta
//
