LOCUS       62056_20035        9290 bp DNA     circular SYN 18-NOV-2021
DEFINITION  Cloning vector pSN.5_mTagBFP2, complete sequence.
ACCESSION   MT891155
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Pasin F, Kulasekaran S, Natale P, Simon-Mateo C, Garcia JA.
  TITLE     Rapid fluorescent reporter quantification by leaf disc analysis and 
            its application in plant-virus studies
  JOURNAL   Plant Methods 10, 22 (2014)
  PUBMED    25053970
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2020) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 9290)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods 10, 22 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2020) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
FEATURES             Location/Qualifiers
     source          1..9290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        407..1076
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1076..1221
                     /label=Plum pox virus 5'UTR
                     /note="Plum pox virus 5'UTR"
     CDS             1222..1905
                     /label=mTagBFP2
                     /note="enhanced monomeric blue fluorescent protein (Subach
                     et al., 2011)"
     misc_feature    1915..1944
                     /label=MYC tag
                     /note="MYC tag"
     3'UTR           2115..2331
                     /label=Plum pox virus 3'UTR
                     /note="Plum pox virus 3'UTR"
     terminator      2413..2665
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2756..2780
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4080..4706
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5143..6207
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6276..6470
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6814..6954
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7140..7728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7975..8763)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attgaattcc tcgagtacgt aggatccatt
      121 taaattctag accagtacgc acgattcaag gcttgcttca caaaccaagg caagtaatag
      181 agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag tcaaagattc
      241 aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata cagagtctct
      301 tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac acacttgtct
      361 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac
      421 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg
      481 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
      541 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
      601 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
      661 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      721 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      781 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      841 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tcctctgccg acagtggtcc
      901 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
      961 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     1021 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggaaaat
     1081 ataaaaactc aacacaacat acaaaatttt aagcaatcaa atcaatctca agctatcaaa
     1141 atttttcaaa tctcacttga aagatcaaaa atcaacaaag aaaatccctt aatttctcta
     1201 ccaaatttac tgcaagtcaa gatgtcatct aagggtgaag agcttatcaa agagaatatg
     1261 cacatgaagt tgtatatgga gggtactgtg gataaccatc actttaaatg tacatcagaa
     1321 ggagagggaa agccttacga gggtactcaa acaatgagaa ttaaggttgt ggagggtggt
     1381 cctcttccat ttgctttcga tatcttggca acatcttttc tctacggttc aaaaactttc
     1441 attaaccata cacagggtat tccagatttc ttcaagcagt cttttccaga gggattcact
     1501 tgggaaagag ttactactta tgaggatggt ggtgttctta ctgcaactca agatacatct
     1561 cttcaggatg gatgcttgat ctataacgtt aagatcagag gagttaattt cacttcaaac
     1621 ggacctgtta tgcaaaagaa aacattgggt tgggaggctt ttactgagac attgtaccca
     1681 gcagatggag gtttggaagg aagaaacgat atggctctta aattggttgg aggttctcat
     1741 ctcatcgcaa atgctaaaac tacatataga tcaaagaaac ctgctaagaa cctcaaaatg
     1801 ccaggagttt attacgtgga ttacagactt gaaagaatta aagaggcaaa caacgaaact
     1861 tatgttgaac agcatgaggt tgctgtggca agatacgcag gactgggagg tggagaacag
     1921 aagttgattt cagaggaaga cttagcgtaa gtgaagctca tatccagatg aaggcagcag
     1981 cattgagaaa tgttcaaaat cgtttatttg gcttggatgg aaacgtcgga acacaagaag
     2041 aggacacaga gagacacacc gctggtgatg ttaatcgcaa catgcacaac ctcctcggtg
     2101 tgaggggagt gtagtggtct cggtatctat cataaactct acctgggtga gagtctaatc
     2161 atccagttgt ttttagattc ctgttagcat ccttttctcc gctttaatag cagtacattc
     2221 agtgaggttt tacctccata tgttctagtc tgttattgtc gaacacaggc ccttgtatct
     2281 gatgtagcga gtgcttcact ccattcgggt tatagttctt gtgcaagaga caaaaaaaaa
     2341 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2401 tgcatgcctg cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     2461 gccggtcttg cgatgattat catctaattt ctgttgaatt acgttaagca tgtaataatt
     2521 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     2581 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     2641 gcggtgtcat ctatgttact cgatctctag cttgagcttg gatcagattg tcgtttccta
     2701 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     2761 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa
     2821 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     2881 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     2941 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     3001 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     3061 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     3121 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     3181 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     3241 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     3301 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     3361 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     3421 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     3481 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     3541 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     3601 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     3661 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     3721 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     3781 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     3841 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     3901 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     3961 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     4021 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     4081 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     4141 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     4201 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     4261 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     4321 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     4381 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     4441 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     4501 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     4561 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     4621 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     4681 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     4741 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     4801 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     4861 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     4921 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     4981 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     5041 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
     5101 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa accatccggc
     5161 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     5221 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     5281 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     5341 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     5401 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     5461 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     5521 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     5581 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     5641 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     5701 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     5761 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     5821 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     5881 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     5941 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     6001 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     6061 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     6121 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     6181 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     6241 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
     6301 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     6361 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     6421 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     6481 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     6541 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     6601 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     6661 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     6721 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     6781 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     6841 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6901 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     6961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7021 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7321 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7561 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7621 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7741 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7801 atgcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     7861 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaatcgctt
     7921 gagttaacgc cggcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc
     7981 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
     8041 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     8101 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     8161 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     8221 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     8281 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     8341 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     8401 gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     8461 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
     8521 ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     8581 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
     8641 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg
     8701 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc
     8761 catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca
     8821 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     8881 ctgtacccca aaaaaacatg tcataacaag aagccatgaa aaccgccact gcgccgttac
     8941 caccgctgcg ttcggtcaag gttctggacc agttgcgtga cggcagttac gctacttgca
     9001 ttacagctta cgaaccgaac gaggcttatg tccactgggt tcgtgcccga attgatcaca
     9061 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     9121 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     9181 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     9241 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
//
