LOCUS       62056_20040        9326 bp DNA     circular SYN 18-NOV-2021
DEFINITION  Cloning vector pSN.5_mTFP1, complete sequence.
ACCESSION   MT891156
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Pasin F, Kulasekaran S, Natale P, Simon-Mateo C, Garcia JA.
  TITLE     Rapid fluorescent reporter quantification by leaf disc analysis and 
            its application in plant-virus studies
  JOURNAL   Plant Methods 10, 22 (2014)
  PUBMED    25053970
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2020) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 9326)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods 10, 22 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2020) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
FEATURES             Location/Qualifiers
     source          1..9326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        407..1076
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1076..1221
                     /label=Plum pox virus 5'UTR
                     /note="Plum pox virus 5'UTR"
     CDS             1222..1929
                     /label=mTFP1
                     /note="monomeric teal (cyan) fluorescent protein (Ai et
                     al., 2006)"
     CDS             1930..1944
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     CDS             1954..1977
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     3'UTR           2151..2367
                     /label=Plum pox virus 3'UTR
                     /note="Plum pox virus 3'UTR"
     terminator      2449..2701
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2792..2816
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4116..4742
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5179..6243
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6312..6506
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6850..6990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7176..7764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8011..8799)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attgaattcc tcgagtacgt aggatccatt
      121 taaattctag accagtacgc acgattcaag gcttgcttca caaaccaagg caagtaatag
      181 agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag tcaaagattc
      241 aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata cagagtctct
      301 tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac acacttgtct
      361 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac
      421 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg
      481 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
      541 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
      601 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
      661 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      721 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      781 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      841 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tcctctgccg acagtggtcc
      901 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
      961 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     1021 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggaaaat
     1081 ataaaaactc aacacaacat acaaaatttt aagcaatcaa atcaatctca agctatcaaa
     1141 atttttcaaa tctcacttga aagatcaaaa atcaacaaag aaaatccctt aatttctcta
     1201 ccaaatttac tgcaagtcaa gatggtttct aaaggtgaag agactacaat gggagtgatc
     1261 aagcctgata tgaagatcaa gctcaagatg gaagggaacg tgaacggaca cgctttcgtt
     1321 attgaaggag aaggggaggg caagccttac gatggaacta acaccatcaa ccttgaagtg
     1381 aaagagggtg ctcctctccc tttctcttac gatatcctca ctaccgcttt cgcttacgga
     1441 aacagggctt tcaccaagta ccctgatgat atccctaact acttcaagca gtctttccca
     1501 gagggatact cttgggagag aaccatgacc ttcgaggata agggaatcgt gaaagtgaag
     1561 tctgatatct ctatggaaga ggattctttc atctacgaga tccatctcaa gggcgagaac
     1621 ttccctccta acggacctgt gatgcagaaa aagactaccg gatgggatgc ttctaccgag
     1681 cggatgtatg tgagagatgg tgtgttgaag ggtgatgtga agcacaagct gttgcttgag
     1741 ggtggtggac atcacagagt ggatttcaag accatctaca gggctaagaa ggctgttaag
     1801 ctccctgatt accacttcgt ggatcatcga atcgagatcc tcaaccacga taaggattac
     1861 aacaaagtga ccgtgtacga gtctgctgtg gctagaaact ctaccgatgg aggctctggt
     1921 gattacaaag acgatgacga taagggcagt ggagactata aggatgacga cgataaagga
     1981 gcataagtga agctcatatc cagatgaagg cagcagcatt gagaaatgtt caaaatcgtt
     2041 tatttggctt ggatggaaac gtcggaacac aagaagagga cacagagaga cacaccgctg
     2101 gtgatgttaa tcgcaacatg cacaacctcc tcggtgtgag gggagtgtag tggtctcggt
     2161 atctatcata aactctacct gggtgagagt ctaatcatcc agttgttttt agattcctgt
     2221 tagcatcctt ttctccgctt taatagcagt acattcagtg aggttttacc tccatatgtt
     2281 ctagtctgtt attgtcgaac acaggccctt gtatctgatg tagcgagtgc ttcactccat
     2341 tcgggttata gttcttgtgc aagagacaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2401 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaatgca tgcctgcaga tcgttcaaac
     2461 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcatc
     2521 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     2581 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     2641 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactcgat
     2701 ctctagcttg agcttggatc agattgtcgt ttcctagctt gagcttggat cagattgtcg
     2761 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     2821 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
     2881 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     2941 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     3001 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     3061 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     3121 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     3181 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     3241 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     3301 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     3361 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     3421 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     3481 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     3541 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     3601 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     3661 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     3721 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     3781 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     3841 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     3901 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     3961 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     4021 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     4081 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     4141 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     4201 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     4261 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     4321 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     4381 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     4441 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     4501 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     4561 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     4621 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     4681 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     4741 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     4801 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     4861 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     4921 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     4981 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     5041 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     5101 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     5161 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     5221 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     5281 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     5341 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     5401 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     5461 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     5521 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     5581 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     5641 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     5701 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     5761 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     5821 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     5881 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     5941 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     6001 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     6061 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     6121 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     6181 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     6241 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     6301 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     6361 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     6421 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     6481 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     6541 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     6601 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     6661 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     6721 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6781 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6841 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     6901 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     6961 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     7021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7081 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7141 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7201 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7261 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7321 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7381 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7441 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7501 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7561 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7621 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7681 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7741 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7801 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc atgatatatc tcccaatttg
     7861 tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg atagtttggc
     7921 tgtgagcaat tatgtgctta gtgcatctaa tcgcttgagt taacgccggc gaagcggcgt
     7981 cggcttgaac gaatttctag ctagacatta tttgccgact accttggtga tctcgccttt
     8041 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     8101 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     8161 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     8221 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     8281 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     8341 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     8401 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     8461 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     8521 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     8581 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     8641 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     8701 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     8761 tagttgagtc gatacttcgg cgatcaccgc ttcccccatg atgtttaact ttgttttagg
     8821 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     8881 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacatgtcat
     8941 aacaagaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     9001 tggaccagtt gcgtgacggc agttacgcta cttgcattac agcttacgaa ccgaacgagg
     9061 cttatgtcca ctgggttcgt gcccgaattg atcacaggca gcaacgctct gtcatcgtta
     9121 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
     9181 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
     9241 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     9301 cggtcgggga gctgttggct ggctgg
//
