LOCUS       62056_20025        9272 bp DNA     circular SYN 18-NOV-2021
DEFINITION  Cloning vector pSN.5_mNeon, complete sequence.
ACCESSION   MT891153
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Pasin F, Kulasekaran S, Natale P, Simon-Mateo C, Garcia JA.
  TITLE     Rapid fluorescent reporter quantification by leaf disc analysis and 
            its application in plant-virus studies
  JOURNAL   Plant Methods 10, 22 (2014)
  PUBMED    25053970
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2020) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods 10, 22 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2020) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        407..1076
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1076..1221
                     /label=Plum pox virus 5'UTR
                     /note="Plum pox virus 5'UTR"
     CDS             1222..1929
                     /label=mNeonGreen
                     /note="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
     3'UTR           2097..2313
                     /label=Plum pox virus 3'UTR
                     /note="Plum pox virus 3'UTR"
     terminator      2395..2647
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2738..2762
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4062..4688
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5125..6189
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6258..6452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6796..6936
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7122..7710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7957..8745)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attgaattcc tcgagtacgt aggatccatt
      121 taaattctag accagtacgc acgattcaag gcttgcttca caaaccaagg caagtaatag
      181 agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag tcaaagattc
      241 aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata cagagtctct
      301 tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac acacttgtct
      361 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac
      421 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg
      481 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
      541 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
      601 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
      661 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      721 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      781 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      841 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tcctctgccg acagtggtcc
      901 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
      961 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     1021 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggaaaat
     1081 ataaaaactc aacacaacat acaaaatttt aagcaatcaa atcaatctca agctatcaaa
     1141 atttttcaaa tctcacttga aagatcaaaa atcaacaaag aaaatccctt aatttctcta
     1201 ccaaatttac tgcaagtcaa gatggtgagc aagggagagg aggataacat ggcctctctc
     1261 ccagctacac atgagcttca catctttgga tccatcaacg gtgtggactt tgacatggtg
     1321 ggtcagggaa ccggaaatcc aaatgatgga tatgaggagc ttaaccttaa gtccaccaag
     1381 ggtgacctcc agttctcccc atggattctt gtccctcata tcggatatgg attccatcag
     1441 taccttcctt accctgatgg aatgtctccg tttcaagccg caatggttga tggatccgga
     1501 taccaagtcc atagaacaat gcagtttgaa gatggtgcct cccttactgt taactacaga
     1561 tacacctacg agggaagcca catcaaagga gaggcccagg tgaagggaac tggtttccct
     1621 gctgacggtc ctgtgatgac caactctctt accgctgctg actggtgcag gtctaagaaa
     1681 acttacccta acgacaaaac catcatcagt acctttaagt ggagttacac cactggaaat
     1741 ggcaagagat acagaagcac tgctagaacc acctacacct ttgccaagcc aatggctgct
     1801 aactatctta agaaccagcc tatgtacgtg ttccgtaaga ctgagctcaa gcactccaag
     1861 accgagctca acttcaagga gtggcaaaag gcctttaccg atgtgatggg aatggacgag
     1921 ctatacaaat aagtgaagct catatccaga tgaaggcagc agcattgaga aatgttcaaa
     1981 atcgtttatt tggcttggat ggaaacgtcg gaacacaaga agaggacaca gagagacaca
     2041 ccgctggtga tgttaatcgc aacatgcaca acctcctcgg tgtgagggga gtgtagtggt
     2101 ctcggtatct atcataaact ctacctgggt gagagtctaa tcatccagtt gtttttagat
     2161 tcctgttagc atccttttct ccgctttaat agcagtacat tcagtgaggt tttacctcca
     2221 tatgttctag tctgttattg tcgaacacag gcccttgtat ctgatgtagc gagtgcttca
     2281 ctccattcgg gttatagttc ttgtgcaaga gacaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2341 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aatgcatgcc tgcagatcgt
     2401 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2461 atcatctaat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2521 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2581 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2641 ctcgatctct agcttgagct tggatcagat tgtcgtttcc tagcttgagc ttggatcaga
     2701 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
     2761 acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt
     2821 atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac
     2881 tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc
     2941 ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt
     3001 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
     3061 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
     3121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
     3181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
     3241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
     3301 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
     3361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
     3421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
     3481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
     3541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
     3601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
     3661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
     3721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
     3781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
     3841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
     3901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
     3961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     4021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     4081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     4141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     4201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     4261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     4321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     4381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     4441 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     4501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     4561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     4621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     4681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     4741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     4801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     4861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     4921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     4981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     5041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     5101 ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg
     5161 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     5221 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     5281 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     5341 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     5401 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     5461 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     5521 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     5581 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     5641 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     5701 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     5761 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     5821 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     5881 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     5941 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     6001 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     6061 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     6121 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     6181 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     6241 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     6301 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     6361 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     6421 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     6481 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     6541 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     6601 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     6661 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     6721 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     6781 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     6841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     6901 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     6961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7561 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc
     7801 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     7861 tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac gccggcgaag
     7921 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc
     7981 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     8041 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     8101 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     8161 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     8221 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     8281 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     8341 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     8401 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     8461 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     8521 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     8581 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     8641 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     8701 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt
     8761 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     8821 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     8881 tgtcataaca agaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     8941 aggttctgga ccagttgcgt gacggcagtt acgctacttg cattacagct tacgaaccga
     9001 acgaggctta tgtccactgg gttcgtgccc gaattgatca caggcagcaa cgctctgtca
     9061 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
     9121 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
     9181 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     9241 agctgccggt cggggagctg ttggctggct gg
//
