LOCUS       62056_20045        9283 bp DNA     circular SYN 18-NOV-2021
DEFINITION  Cloning vector pSN.5_TagRFP-T, complete sequence.
ACCESSION   MT891157
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Pasin F, Kulasekaran S, Natale P, Simon-Mateo C, Garcia JA.
  TITLE     Rapid fluorescent reporter quantification by leaf disc analysis and 
            its application in plant-virus studies
  JOURNAL   Plant Methods 10, 22 (2014)
  PUBMED    25053970
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2020) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 9283)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods 10, 22 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2020) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
FEATURES             Location/Qualifiers
     source          1..9283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        407..1076
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1076..1221
                     /label=Plum pox virus 5'UTR
                     /note="Plum pox virus 5'UTR"
     CDS             1222..1932
                     /label=TagRFP-T
                     /note="photostable variant of TagRFP (Shaner et al., 2008)"
     3'UTR           2108..2324
                     /label=Plum pox virus 3'UTR
                     /note="Plum pox virus 3'UTR"
     terminator      2406..2658
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2749..2773
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4073..4699
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5136..6200
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6269..6463
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6807..6947
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7133..7721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7968..8756)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attgaattcc tcgagtacgt aggatccatt
      121 taaattctag accagtacgc acgattcaag gcttgcttca caaaccaagg caagtaatag
      181 agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag tcaaagattc
      241 aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata cagagtctct
      301 tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac acacttgtct
      361 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac
      421 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg
      481 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
      541 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
      601 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
      661 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      721 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      781 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      841 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tcctctgccg acagtggtcc
      901 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
      961 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     1021 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggaaaat
     1081 ataaaaactc aacacaacat acaaaatttt aagcaatcaa atcaatctca agctatcaaa
     1141 atttttcaaa tctcacttga aagatcaaaa atcaacaaag aaaatccctt aatttctcta
     1201 ccaaatttac tgcaagtcaa gatggtttca aagggagaag agctgattaa ggagaacatg
     1261 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag
     1321 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc
     1381 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag cagaaccttc
     1441 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca
     1501 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
     1561 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac
     1621 ggccctgtga tgcagaagaa aacactcggc tgggaggcca acaccgagat gctgtacccc
     1681 gctgacggcg gcctggaagg cagaacagac atggccctga agctcgtggg cgggggccac
     1741 ctgatctgca acttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
     1801 cccggcgtct actatgtgga ccacagactg gaaagaatca aggaggccga caaagagacc
     1861 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
     1921 cacaaactta actaatcgat cacgtgaagc tcatatccag atgaaggcag cagcattgag
     1981 aaatgttcaa aatcgtttat ttggcttgga tggaaacgtc ggaacacaag aagaggacac
     2041 agagagacac accgctggtg atgttaatcg caacatgcac aacctcctcg gtgtgagggg
     2101 agtgtagtgg tctcggtatc tatcataaac tctacctggg tgagagtcta atcatccagt
     2161 tgtttttaga ttcctgttag catccttttc tccgctttaa tagcagtaca ttcagtgagg
     2221 ttttacctcc atatgttcta gtctgttatt gtcgaacaca ggcccttgta tctgatgtag
     2281 cgagtgcttc actccattcg ggttatagtt cttgtgcaag agacaaaaaa aaaaaaaaaa
     2341 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaatgcatgc
     2401 ctgcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     2461 ttgcgatgat tatcatctaa tttctgttga attacgttaa gcatgtaata attaacatgt
     2521 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     2581 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     2641 catctatgtt actcgatctc tagcttgagc ttggatcaga ttgtcgtttc ctagcttgag
     2701 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     2761 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
     2821 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     2881 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     2941 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     3001 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     3061 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     3121 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     3181 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     3241 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     3301 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     3361 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     3421 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     3481 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     3541 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     3601 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     3661 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     3721 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     3781 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     3841 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     3901 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     3961 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     4021 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     4081 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     4141 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     4201 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     4261 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     4321 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     4381 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     4441 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     4501 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     4561 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     4621 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     4681 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     4741 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     4801 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     4861 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     4921 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     4981 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     5041 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
     5101 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
     5161 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     5221 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     5281 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     5341 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     5401 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     5461 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     5521 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     5581 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     5641 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     5701 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     5761 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     5821 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     5881 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     5941 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     6001 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     6061 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     6121 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     6181 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     6241 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     6301 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     6361 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     6421 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     6481 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     6541 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     6601 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     6661 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6721 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6781 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     6841 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6901 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     6961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7561 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7741 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg
     7801 atatatctcc caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact
     7861 tgacctgata gtttggctgt gagcaattat gtgcttagtg catctaatcg cttgagttaa
     7921 cgccggcgaa gcggcgtcgg cttgaacgaa tttctagcta gacattattt gccgactacc
     7981 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
     8041 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
     8101 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
     8161 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
     8221 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
     8281 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
     8341 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
     8401 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
     8461 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
     8521 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
     8581 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
     8641 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
     8701 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc ccccatgatg
     8761 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     8821 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
     8881 ccaaaaaaac atgtcataac aagaagccat gaaaaccgcc actgcgccgt taccaccgct
     8941 gcgttcggtc aaggttctgg accagttgcg tgacggcagt tacgctactt gcattacagc
     9001 ttacgaaccg aacgaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca
     9061 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
     9121 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
     9181 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg
     9241 attttgtgcc gagctgccgg tcggggagct gttggctggc tgg
//
