LOCUS       62056_20020        5370 bp DNA     circular SYN 03-MAR-2020
DEFINITION  Cloning vector pSN-caSAT1, complete sequence.
ACCESSION   MT001914
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-JAN-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      335..832
                     /label=Candida albicans ACT1 promoter
                     /note="Candida albicans ACT1 promoter"
                     /regulatory_class="promoter"
     gene            833..2059
                     /gene="caSAT1"
                     /label=caSAT1
     CDS             join(833..842,1497..2059)
                     /codon_start=1
                     /transl_table=11
                     /gene="caSAT1"
                     /product="streptothricin acetyltransferase"
                     /label=caSAT1
                     /note="codon-optimized for Candida albicans"
                     /protein_id="QID92303.1"
                     /translation="MDGEEVAALVIDNGSHMKISVIPEQVAETLDAENHFIVREVFDVH
                     LSDQGFELSTRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEH
                     IVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDL
                     FTYKTRPQVSNETAMYWYWFSGAQDDA"
     regulatory      2063..2192
                     /label=Candida albicans URA3 terminator
                     /note="Candida albicans URA3 terminator"
                     /regulatory_class="terminator"
     promoter        complement(2258..2276)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2283..2299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2437..2736
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             3088..3879
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4089..4460
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      4601..5189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc cgagcgtcaa aactagagaa taataaagaa
      361 aacgatcttt tcaaaaagaa aaaacctttt agttttcctt tgttgttgtt gtgggtgtgt
      421 gctatttata ttatatagtt tactcataat accataaaat attcggtttg attaggttat
      481 tttaataagc taatttgttt ctaatcgtgt aatttatgct gtgtatatta agtagtgtgt
      541 gcactgccca aaaatgtttg ttgtttatag tcggttaaag agaaaaaaga aaaaaagatc
      601 catacacaca cgttaattag ttgttcaacg taatacactc atattttgtt cttatttgct
      661 ttcggtcgct gttctcacca agatttattg ccaacgaaac aatttttttt tatatatttt
      721 cagatttttc tttttttcct ttcctttcct tttctaattt tcactcctgg ttttctttct
      781 ttcttagaaa cattatctcg atattaatat taaaaaaata taatcattca aaatggacgg
      841 tggtatgttt tagtttagct tcaattctaa ttgattgatt aatcagttga ttggtttcaa
      901 tatgacaaat gggtagggtg ggaaaacttc attttcaatt cagatcaaac ttttttgttg
      961 tcgacataat atttctcgtt tgggatgtta ctgtcacatt aataatacac acacatcagc
     1021 ttataatttt gaaagtaatt tatcagatat gttgtgacga tcagtggaaa tggctaactt
     1081 caatgtatct gttcttcccc tttttcaaag ttcacgtttt ttgattgatt gattgatctg
     1141 tcggcagtgg tttcaaaacc attcggtgag taatcctatc aatcaatgtt acgacaaaag
     1201 gctcaatatt caaaattgca atgttttatg ttttcctacg tgtacttgtg caaggcaatt
     1261 gattcaacat tgcttttggt gtttgacgag tttctagttt ggacttgtgt tgttatctgg
     1321 gctatacaga tttcccggct cactatgaat tttttttttc gacgctcagt gcacacaact
     1381 ataaacaaca caaacacaaa cacagcaaga aaaaaaaaaa acgaacattg aattgaaacc
     1441 aagccaactg aaaaattcct tatttaaatg actgtcatac taacccattt ttatagaaga
     1501 agttgctgct ttagttatcg ataacggttc tcatatgaaa atttcggtga tccctgagca
     1561 ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt tcgatgtgca
     1621 cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc ggaaggatta
     1681 catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca tcgaccaaga
     1741 gcttgtcggg aagattgaac tcaactcaac atggaacgat ctagcctcta tcgaacacat
     1801 tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg aatttgcgaa
     1861 aaagtgggca ctaagcagac agctccttgg catacgatta gagacacaaa cgaacaatgt
     1921 acctgcctgc aatttgtacg caaaatgtgg ctttactctc ggcggcattg acctcttcac
     1981 gtataaaact agacctcaag tctcgaacga aacagcgatg tactggtact ggttctcggg
     2041 agcacaggat gacgcctaac atatgtgaag tgtgaagggg gagattttca ctttattaga
     2101 tttgtatata tgtataataa ataaataaat aagttaaata aataattaga taagggtggt
     2161 aattattact atttacaatc aaaggtggtc ctggaattct gcagatatcc atcacactgg
     2221 cggccgctcg agcatgcatc tagagggccc aattcgccct atagtgagtc gtattacaat
     2281 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2341 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2401 cgcccttccc aacagttgcg cagcctatac gtacggcagt ttaaggttta cacctataaa
     2461 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgccgggg
     2521 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     2581 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     2641 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     2701 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggcat gagattatca
     2761 aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa acggtgctga
     2821 ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga
     2881 aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca
     2941 gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa
     3001 gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc aagctctgat
     3061 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     3121 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     3181 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     3241 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     3301 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     3361 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     3421 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     3481 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     3541 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     3601 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     3661 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     3721 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     3781 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     3841 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattattaa cgcttacaat
     3901 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacaggtg
     3961 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatag cacgtgagga
     4081 gggccaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     4141 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     4201 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     4261 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     4321 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     4381 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     4441 tcgtggccga ggagcaggac tgacacgtgc taaaacttca tttttaattt aaaaggatct
     4501 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4561 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4621 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4681 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4741 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4801 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4861 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4921 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4981 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5041 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5101 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5161 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5221 tgggcttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5281 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5341 gcagcgagtc agtgagcgag gaagcggaag
//
